Lipid-Binding Studies of Human Apolipoprotein A-I and Its Terminally Truncated Mutants†

Biochemistry ◽  
2003 ◽  
Vol 42 (45) ◽  
pp. 13260-13268 ◽  
Author(s):  
Yiling Fang ◽  
Olga Gursky ◽  
David Atkinson
Keyword(s):  
Lipid Binding ◽  
Binding Studies ◽  
Human Apolipoprotein ◽  
Apolipoprotein A

Lipid-Binding Properties of Synthetic Peptide Fragments of Human Apolipoprotein A-II

1996 ◽  
Vol 242 (3) ◽  
pp. 657-664 ◽  
Author(s):  
Claire Benetollo ◽  
Gilles Lambert ◽  
Corinne Talussot ◽  
Berlinda Vanloo ◽  
Tom Cauteren ◽  
...  
Keyword(s):  
Synthetic Peptide ◽  
Lipid Binding ◽  
Peptide Fragments ◽  
Binding Properties ◽  
Human Apolipoprotein ◽  
Apolipoprotein A

scholarly journals Influence of N-terminal helix bundle stability on the lipid-binding properties of human apolipoprotein A-I

2011 ◽  
Vol 1811 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Masafumi Tanaka ◽  
Padmaja Dhanasekaran ◽  
David Nguyen ◽  
Margaret Nickel ◽  
Yuki Takechi ◽  
...  
Keyword(s):  
Lipid Binding ◽  
Binding Properties ◽  
Helix Bundle ◽  
Human Apolipoprotein ◽  
Apolipoprotein A

Evaluation of lipid-binding properties of the N-terminal helical segments in human apolipoprotein A-I using fragment peptides

2009 ◽  
Vol 15 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Masafumi Tanaka ◽  
Toshitaka Tanaka ◽  
Shinya Ohta ◽  
Toru Kawakami ◽  
Hiroyuki Konno ◽  
...  
Keyword(s):  
Lipid Binding ◽  
Binding Properties ◽  
Human Apolipoprotein ◽  
Apolipoprotein A

Conformation and Lipid Binding of the N-Terminal (1−44) Domain of Human Apolipoprotein A-I†

Biochemistry ◽  
2004 ◽  
Vol 43 (41) ◽  
pp. 13156-13164 ◽  
Author(s):  
Hongli L. Zhu ◽  
David Atkinson
Keyword(s):  
Lipid Binding ◽  
Human Apolipoprotein ◽  
Apolipoprotein A

The Identification and Significance of a Thr→Pro Polymorphism in Kringle IV Type 8 of Apolipoprotein(a)

1997 ◽  
Vol 77 (05) ◽  
pp. 0949-0954 ◽  
Author(s):  
J Prins ◽  
F R Lues ◽  
Y Y van der Hoek ◽  
J J.P Kastelein ◽  
B N Bouma ◽  
...  
Keyword(s):  
Case Control Study ◽  
Amino Acid Position ◽  
Case Control ◽  
Binding Studies ◽  
Binding Characteristics ◽  
Apolipoprotein A ◽  
Significant Independent Risk Factor ◽  
And Gender ◽  
Control Study

SummaryElevated plasma levels of lipoprotein(a) [Lp(a)] represent a significant independent risk factor for the development of atherosclerosis. Interindividual levels of apo(a) vary over 1000-fold and are mainly due to inheritance that is linked to the locus of the apolipoprotein(a) [apo(a)] gene. The apo(a) gene encodes multiple repeats of a sequence exhibiting up to 85% DNA sequence homology with plasminogen kringle IV (K.IV), a lysine binding domain. In our search for sequence polymorphisms in the K.IV coding domain, we identified a polymorphism predicting a Thr→Pro substitution located at amino acid position 12 of kringle IV type 8 of apo(a). The functional and clinical significance of this polymorphism was analysed in a case-control study and by comparing the in vitro lysine binding characteristics of the two Lp(a) subtypes.The case-control study (involving 153 subjects having symptomatic atherosclerosis and 153 age and gender matched normolipidemic controls) revealed an overall allele frequency for the Thr12-→Pro substitution in kringle IV type 8 of 14% and a negative association between presence of the Pro12-subtype and symptomatic atherosclerosis (p <0.03). The in vitro lysine binding studies, using Lp(a) isolated from subjects homozygous for either Thr12 or Pro12 in K.IV type 8, revealed comparable lysine-Sepharose binding fractions for the two subtypes. The binding affinity (Kd) for immobilised plasmin degraded des- AA-fibrin (DesafibTM-X) was also comparable for the two subtypes, however a decreased maximal attainable binding (Bmax) for immobilised desafibTM-X was observed for the Pro12-subtype Lp(a).

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