Modulation of Na,K-ATPase by Phospholipids and Cholesterol. II. Steady-State and Presteady-State Kinetics†

Biochemistry ◽  
2003 ◽  
Vol 42 (28) ◽  
pp. 8541-8549 ◽  
Author(s):  
Flemming Cornelius ◽  
Nigel Turner ◽  
Hanne R. Z. Christensen
Keyword(s):  
Steady State ◽  
Presteady State Kinetics

Presteady-state kinetics of Bacillus 1,3–1,4-β-glucanase: binding and hydrolysis of a 4-methylumbelliferyl trisaccharide substrate

2001 ◽  
Vol 357 (1) ◽  
pp. 195-202
Author(s):  
Mireia ABEL ◽  
Antoni PLANAS ◽  
Ulla CHRISTENSEN
Keyword(s):  
Steady State ◽  
Rate Constant ◽  
Product Formation ◽  
Lag Phase ◽  
Wild Type ◽  
Induced Fit ◽  
Enzyme Substrate ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Presteady State Kinetics

In the present study the first stopped-flow experiments performed on Bacillus 1,3–1,4-β-glucanases are reported. The presteady-state kinetics of the binding of 4-methylumbelliferyl 3-O-β-cellobiosyl-β-d-glucoside to the inactive mutant E134A, and the wild-type-catalysed hydrolysis of the same substrate, were studied by measuring changes in the fluorescence of bound substrate or 4-methylumbelliferone produced. The presteady-state traces all showed an initial lag phase followed by a fast monoexponential phase leading to equilibration (for binding to E134A) or to steady state product formation (for the wild-type reaction). The lag phase, with a rate constant of the order of 100s−1, was independent of the substrate concentration; apparently an induced-fit mechanism governs the formation of enzyme–substrate complexes. The concentration dependencies of the observed rate constant of the second presteady-state phase were analysed according to a number of reaction models. For the reaction of the wild-type enzyme, it is shown that the fast product formation observed before steady state is not due to a rate-determining deglycosylation step. A model that can explain the observed results involves, in addition to the induced fit, a conformational change of the productive ES complex into a form that binds a second substrate molecule in a non-productive mode.

scholarly journals Temperature Dependence of Steady-State and Presteady-State Kinetics of a Type IIb Na+/Pi Cotransporter

2007 ◽  
Vol 215 (2-3) ◽  
pp. 81-92 ◽  
Author(s):  
Andrea Bacconi ◽  
Silvia Ravera ◽  
Leila V. Virkki ◽  
Heini Murer ◽  
Ian C. Forster
Keyword(s):  
Steady State ◽  
Temperature Dependence ◽  
Kinetics Of ◽  
Presteady State Kinetics

Studies on Water in the Hydration Chamber of a Modified Electron Microscope

1970 ◽  
Vol 28 ◽  
pp. 544-545
Author(s):  
R. C. Moretz ◽  
G. G. Hausner ◽  
D. F. Parsons
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Electron Microscope ◽  
Steady State ◽  
Room Temperature ◽  
Small Mass ◽  
Equilibrium Pressure ◽  
Biological Objects ◽  
Mechanical Pump ◽  
Differential Pumping

Use of the electron microscope to examine wet objects is possible due to the small mass thickness of the equilibrium pressure of water vapor at room temperature. Previous attempts to examine hydrated biological objects and water itself used a chamber consisting of two small apertures sealed by two thin films. Extensive work in our laboratory showed that such films have an 80% failure rate when wet. Using the principle of differential pumping of the microscope column, we can use open apertures in place of thin film windows.Fig. 1 shows the modified Siemens la specimen chamber with the connections to the water supply and the auxiliary pumping station. A mechanical pump is connected to the vapor supply via a 100μ aperture to maintain steady-state conditions.

Continuous hydrogenolysis of acetal-stabilized lignin in flow

2021 ◽  
Author(s):  
Wu Lan ◽  
Yuan Peng Du ◽  
Songlan Sun ◽  
Jean Behaghel de Bueren ◽  
Florent Héroguel ◽  
...  
Keyword(s):  
Steady State ◽  
Challenges And Opportunities

We performed a steady state high-yielding depolymerization of soluble acetal-stabilized lignin in flow, which offered a window into challenges and opportunities that will be faced when continuously processing this feedstock.

Network reconstruction based on steady-state data

2008 ◽  
Vol 45 ◽  
pp. 161-176 ◽  
Author(s):  
Eduardo D. Sontag
Keyword(s):  
Steady State ◽  
Reverse Engineering ◽  
Theoretical Method ◽  
Network Reconstruction ◽  
Quasi Steady State ◽  
State Data

This paper discusses a theoretical method for the “reverse engineering” of networks based solely on steady-state (and quasi-steady-state) data.

Steady State Disinfection of Water By Ozone and sonozone

1979 ◽  
Vol 1 (4) ◽  
pp. 13-24
Author(s):  
E. Dahi ◽  
E. Lund
Keyword(s):  
Steady State

Olfactory Modulation of Steady- State Visual Evoked Potential Topography in Comparison with Differences in Odor Sensitivity

2002 ◽  
Vol 16 (2) ◽  
pp. 71-81 ◽  
Author(s):  
Caroline M. Owen ◽  
John Patterson ◽  
Richard B. Silberstein
Keyword(s):  
Steady State ◽  
Visual Evoked Potential ◽  
Evoked Potential ◽  
Behavioral Responses ◽  
Simultaneous Recording ◽  
Odor Concentration ◽  
Odor Detection ◽  
Detection Response ◽  
Response Groups ◽  
Visual Evoked

Summary Research was undertaken to determine whether olfactory stimulation can alter steady-state visual evoked potential (SSVEP) topography. Odor-air and air-only stimuli were used to determine whether the SSVEP would be altered when odor was present. Comparisons were also made of the topographic activation associated with air and odor stimulation, with the view toward determining whether the revealed topographic activity would differentiate levels of olfactory sensitivity by clearly identifying supra- and subthreshold odor responses. Using a continuous respiration olfactometer (CRO) to precisely deliver an odor or air stimulus synchronously with the natural respiration, air or odor (n-butanol) was randomly delivered into the inspiratory airstream during the simultaneous recording of SSVEPs and subjective behavioral responses. Subjects were placed in groups based on subjective odor detection response: “yes” and “no” detection groups. In comparison to air, SSVEP topography revealed cortical changes in response to odor stimulation for both response groups, with topographic changes evident for those unable to perceive the odor, showing the presence of a subconscious physiological odor detection response. Differences in regional SSVEP topography were shown for those who reported smelling the odor compared with those who remained unaware of the odor. These changes revealed olfactory modulation of SSVEP topography related to odor awareness and sensitivity and therefore odor concentration relative to thresholds.

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