Arginine Vasopressin Inhibition of Cyclin D1 Gene Expression Blocks the Cell Cycle and Cell Proliferation in the Mouse Y1 Adrenocortical Tumor Cell Line†

Biochemistry ◽  
2003 ◽  
Vol 42 (7) ◽  
pp. 2116-2121 ◽  
Author(s):  
Telma T. Schwindt ◽  
Fábio L. Forti ◽  
Maria Ap. Juliano ◽  
Luiz Juliano ◽  
Hugo A. Armelin
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Tumor Cell ◽  
Cyclin D1 ◽  
Cell Line ◽  
Arginine Vasopressin ◽  
Tumor Cell Line ◽  
Adrenocortical Tumor

Effects of TGFβ1 on gene expression in the HP75 human pituitary tumor cell line identified by gene expression profiling

Endocrine ◽  
2008 ◽  
Vol 33 (1) ◽  
pp. 62-76 ◽  
Author(s):  
Katharina H. Ruebel ◽  
Alexey A. Leontovich ◽  
Yoshinori Tanizaki ◽  
Long Jin ◽  
Gail A. Stilling ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Gene Expression Profiling ◽  
Cell Line ◽  
Expression Profiling ◽  
Pituitary Tumor ◽  
Tumor Cell Line ◽  
Human Pituitary ◽  
Human Pituitary Tumor ◽  
Pituitary Tumor Cell

HETEROGENOUS EXPRESSION OF PLASMINOGEN ACTIVATOR (PA) GENES IN THE HUMAN SARCOMA CELL LINE HT1080

1987 ◽  
Author(s):  
Walter E Laug
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Tissue Type ◽  
Gene Probe ◽  
Ht1080 Cell ◽  
Simultaneous Production ◽  
Tissue Invasion ◽  
Ht1080 Cells

Tumor cell derived PA activities are of crucial importance for tissue invasion and destruction by tumor cells. Therefore, we studied the expression of the PA genes in HT1080 cells using immunoenzymatic methods and specific PA gene probes.Immunenzymatic methods allowed only for the detection of urokinase like PA (u-PA) activities in HT1080 cells which was suppressed by treatment of the cells with dexamethasone (10-7 m). Despite the lack of u-PA activities, the cells still degraded extracellular tissue glycoproteins. Northern blot analysis with specific PA gene probe showed that HT1080 cells express both tissue type PA (t-PA) and u-PA. The enzymatic activities of t-PA were most likely masked by the simultaneous production of inhibitors of PA (PAI). Treatment of HT1080 cells with dexamethasone resulted in increased transcription of t-PA and decreased expression of the u-PA gene, explaining the unchanged tissue destruction by dexamethasone treated HT1080 cells.Cell clones secreting either large or small amounts of enzymatic PA activities were isolated from the parental HT1080 cell line using a fibrin agarose overlay technique.The expression of the u-PA gene was enhanced in high secreting PA clones compared to low secreting PA clones when analyzed on Northern blots. This heterogenous expression of the u-PA gene within the HT1080 cell line was confirmed by in situ hybridization with a specific u-PA gene probe.These findings demonstrate that PA gene expression can be missed with immunenzymatic methods due to simultaneous production of inhibitors of PA. In addition our results show that the expression of a given PA gene may be heterogenous on the cellular level within an established tumor cell line. These findings, therefore, suggest cellular variation of PA gene expression in tumor which may be of fundamental importance for tissue invasion and metastasis by cancer cells.

scholarly journals CisModification of the Steroid 21-Hydroxylase Gene Prevents Its Expression in the Y1 Mouse Adrenocortical Tumor Cell Line

1990 ◽  
Vol 4 (8) ◽  
pp. 1144-1152 ◽  
Author(s):  
Moshe Szyf ◽  
David S. Milstone ◽  
Bernard P. Schimmer ◽  
Keith L. Parker ◽  
J. G. Seidman
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Adrenocortical Tumor ◽  
Hydroxylase Gene

Molecular and Functional Effects of the Novel MEK Inhibitor PD0325901 in Preclinical Models of Human Leukemias.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 254-254
Author(s):  
Michele Milella ◽  
Maria Rosaria Ricciardi ◽  
Chiara Gregorj ◽  
Fabiana De Cave ◽  
Steven L. Abrams ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Line ◽  
Cell Lines ◽  
Therapeutic Target ◽  
Hematological Malignancies ◽  
Mek Inhibitor ◽  
Sensitive Cell ◽  
Fold Reduction

Abstract The Raf/MEK/ERK signaling module plays a pivotal role in the regulation of cell proliferation, survival, and differentiation. Our group, among others, has recently demonstrated that this pathway is frequently dysregulated in hematological malignancies and may constitute an attractive therapeutic target, particularly in AML. Here we investigated the effects of PD0325901, a novel MEK inhibitor, on phospho-protein expression, gene expression profiles, cell proliferation, and apoptosis in cell line models of AML, ALL, multiple myeloma (MM), ex vivo-cultured primary AML blasts, and oncogene-transformed hematopoietic cells. AML cell lines (OCI-AML2, OCI-AML3, HL-60) were strikingly sensitive to PD0325901 (IC50: 5–19 nM), NB4 (APL) and U266 (MM) showed intermediate sensitivity (IC50: 822 and 724 nM), while all the lymphoid cell lines tested and the myeloid cell lines U937 and KG1 were resistant (IC50 > 1000 nM). Cell growth inhibition was due to inhibition of cell cycle progression and induction of apoptosis. A statistically significant reduction in the proportion of S-phase cells (p=0.01) and increase in the percentage of apoptotic cells (p=0.019) was also observed in 18 primary AML samples in response to 100 nM PD0325901. Analysis of the correlation between sensitivity/resistance to PD0325901 and Ras/Raf mutation status is currently ongoing. PD0325901 effects were also examined in a panel of IL-3-dependent murine myeloid FDC-P1 cell lines transformed to grow in response to 11 different oncogenes in the absence of IL-3. Fms-, Ras-, Raf-1-, B-Raf-, MEK1-, IGF-1R-, and STAT5a-transformed FDC-P1 cells were very sensitive to PD0325901 (IC50: ~ 1 nM), while A-Raf-, BCR-ABL-, EGFR- or Src-transformed cells were 10 to 100 fold less sensitive (IC50: 10 to 100 nM); the parental, IL-3 dependent FDC-P1 cell line had an IC50 > 1000 nM. Analysis of the phosphorylation levels of 18 different target proteins after treatment with 10 nM PD0325901 showed a 5- to 8-fold reduction in ERK-1/2, observed only in sensitive cell lines, and a 2-fold reduction in JNK and STAT3 phosphorylation. PD0325901 (10 nM) treatment also profoundly altered the gene expression profile of the sensitive cell line OCI-AML3: 96 genes were modulated after 24 h (37 up- and 59 down-regulated), most of which involved in cell cycle regulation. Changes in cyclin D1 and D3, cyclin E, and cdc 25A were also validated at the protein level. Overall, PD0325901 shows potent growth-inhibitory and pro-apoptotic activity, indicating that MEK may be an appropriate therapeutic target in an array of different hematological malignancies. Further preclinical/clinical development of this compound is warranted, particularly in myeloid leukemias.

ACTH-receptor deficient mutants of the Y1 mouse adrenocortical tumor cell line

1995 ◽  
Vol 21 (1-2) ◽  
pp. 139-156 ◽  
Author(s):  
Bernard P. Schimmer ◽  
Wai King Kwan ◽  
Jennivine Tsao ◽  
Rong Qiu
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Tumor Cell Line ◽  
Adrenocortical Tumor ◽  
Acth Receptor

scholarly journals Digital Image Analysis Applied to Tumor Cell Proliferation, Aggressiveness, and Migration-Related Protein Synthesis in Neuroblastoma 3D Models

2020 ◽  
Vol 21 (22) ◽  
pp. 8676 ◽  
Author(s):  
Ezequiel Monferrer ◽  
Sabina Sanegre ◽  
Susana Martín-Vañó ◽  
Andrea García-Lizarribar ◽  
Rebeca Burgos-Panadero ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Image Analysis ◽  
Tumor Cell ◽  
Cell Line ◽  
Digital Image ◽  
Digital Image Analysis ◽  
Neuroblastoma Cell ◽  
Tumor Cell Line ◽  
3D Models ◽  
And Migration

Patient-derived cancer 3D models are a promising tool that will revolutionize personalized cancer therapy but that require previous knowledge of optimal cell growth conditions and the most advantageous parameters to evaluate biomimetic relevance and monitor therapy efficacy. This study aims to establish general guidelines on 3D model characterization phenomena, focusing on neuroblastoma. We generated gelatin-based scaffolds with different stiffness and performed SK-N-BE(2) and SH-SY5Y aggressive neuroblastoma cell cultures, also performing co-cultures with mouse stromal Schwann cell line (SW10). Model characterization by digital image analysis at different time points revealed that cell proliferation, vitronectin production, and migration-related gene expression depend on growing conditions and are specific to the tumor cell line. Morphometric data show that 3D in vitro models can help generate optimal patient-derived cancer models, by creating, identifying, and choosing patterns of clinically relevant artificial microenvironments to predict patient tumor cell behavior and therapeutic responses.

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