scholarly journals 2‘-O-[2-(Methylthio)ethyl]-Modified Oligonucleotide:  An Analogue of 2‘-O-[2-(Methoxy)-ethyl]-Modified Oligonucleotide with Improved Protein Binding Properties and High Binding Affinity to Target RNA†

Biochemistry ◽  
2002 ◽  
Vol 41 (39) ◽  
pp. 11642-11648 ◽  
Author(s):  
Thazha P. Prakash ◽  
Muthiah Manoharan ◽  
Andrew M. Kawasaki ◽  
Allister S. Fraser ◽  
Elena A. Lesnik ◽  
...  
Keyword(s):  
Protein Binding ◽  
Binding Affinity ◽  
Binding Properties ◽  
High Binding ◽  
Target Rna ◽  
Modified Oligonucleotide ◽  
High Binding Affinity

scholarly journals Effect of Polymerization Time on the Binding Properties of Ciprofloxacin-Imprinted nanoMIPs Prepared by Solid-Phase Synthesis

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2656
Author(s):  
Matteo Charello ◽  
Laura Anfossi ◽  
Simone Cavalera ◽  
Fabio Di Nardo ◽  
Fiora Artusio ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Polymer Chains ◽  
Polymerization Time ◽  
Dissociation Kinetics ◽  
Binding Properties ◽  
Phase Synthesis ◽  
High Binding ◽  
High Binding Affinity

An innovative approach to imprinted nanoparticles (nanoMIPs) is represented by solid-phase synthesis. Since the polymeric chains grow over time and rearrange themselves around the template, the binding properties of nanoMIPs could depend on the polymerization time. Here we present an explorative study about the effect of different polymerization times on the binding properties of ciprofloxacin-imprinted nanoMIPs. The binding properties towards ciprofloxacin were studied by measuring the binding affinity constants (Keq) and the kinetic rate constants (kd, ka). Furthermore, selectivity and nonspecific binding were valued by measuring the rebinding of levofloxacin onto ciprofloxacin-imprinted nanoMIPs and ciprofloxacin onto diclofenac-imprinted nanoMIPs, respectively. The results show that different polymerization times produce nanoMIPs with different binding properties: short polymerization times (15 min) produced nanoMIPs with high binding affinity but low selectivity (Keq > 107 mol L−1, α ≈ 1); medium polymerization times (30 min–2 h) produced nanoMIPs with high binding affinity and selectivity (Keq ≥ 106 mol L−1, α < 1); and long polymerization times (>2 h) produced nanoMIPs with low binding affinity, fast dissociation kinetics and low selectivity (Keq ≤ 106 mol L−1, kdis > 0.2 min−1, α ≈ 1). The results can be explained as the combined effect of rearrangement and progressive stiffening of the polymer chains around the template molecules.

scholarly journals Herb-target virtual screening and network pharmacology for prediction of molecular mechanism of Danggui Beimu Kushen Wan for prostate cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hong Li ◽  
Andrew Hung ◽  
Angela Wei Hong Yang
Keyword(s):  
Prostate Cancer ◽  
Binding Affinity ◽  
Molecular Mechanisms ◽  
Biological Activities ◽  
Tight Binding ◽  
Experimental Studies ◽  
Network Pharmacology ◽  
High Morbidity ◽  
High Binding ◽  
High Binding Affinity

AbstractProstate cancer (PCa) is a cancer that occurs in the prostate with high morbidity and mortality. Danggui Beimu Kushen Wan (DBKW) is a classic formula for patients with difficult urination including PCa. This study aimed to investigate the molecular mechanisms of DBKW for PCa. We obtained DBKW compounds from our previous reviews. We identified potential targets for PCa from literature search, currently approved drugs and Open Targets database and filtered them by protein–protein interaction network analysis. We selected 26 targets to predict three cancer-related pathways. A total of 621 compounds were screened via molecular docking using PyRx and AutoDock Vina against 21 targets for PCa, producing 13041 docking results. The binding patterns and positions showed that a relatively small number of tight-binding compounds from DBKW were predicted to interact strongly and selectively with three targets. The top five high-binding-affinity compounds were selected to generate a network, indicating that compounds from all three herbs had high binding affinity against the 21 targets and may have potential biological activities with the targets. DBKW contains multi-targeting agents that could act on more than one pathway of PCa simultaneously. Further studies could focus on validating the computational results via experimental studies.

A fluorescent probe for butyrylcholinesterase activity in human serum based on a fluorophore with specific binding affinity for human serum albumin

2019 ◽  
Vol 55 (97) ◽  
pp. 14574-14577 ◽  
Author(s):  
Soyeon Yoo ◽  
Min Su Han
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescent Probe ◽  
Binding Affinity ◽  
Specific Binding ◽  
Turn On ◽  
High Binding ◽  
Butyrylcholinesterase Activity ◽  
High Binding Affinity

We report a novel turn-on sensing probe for the detection of butyrylcholinesterase activity in human serum using a fluorophore with high binding affinity for HSA.

LB50053: A 5-Hydroxytryptamine1A Agent with a High Binding Affinity and a Potency Evoking a K+ Current

Pharmacology ◽  
2002 ◽  
Vol 65 (4) ◽  
pp. 175-181
Author(s):  
Han-Seop Kim ◽  
Taesup Cho ◽  
Changho Lee ◽  
Hyun Joo ◽  
Seung Kim ◽  
...  
Keyword(s):  
Binding Affinity ◽  
High Binding ◽  
High Binding Affinity

scholarly journals Peptide-binding induced inhibition of chemokine CXCL12

RSC Advances ◽  
2017 ◽  
Vol 7 (34) ◽  
pp. 21298-21307
Author(s):  
Hongyang Duan ◽  
Ling Zhu ◽  
Jiaxi Peng ◽  
Mo Yang ◽  
Hanyi Xie ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Peptide Binding ◽  
Inhibitory Effect ◽  
High Binding ◽  
Significant Inhibitory Effect ◽  
Chemokine Cxcl12 ◽  
Cxcr4 Axis ◽  
High Binding Affinity

A designed peptide (W4) has a significant inhibitory effect on the CXCL12/CXCR4 axis by targeting CXCL12 with high binding affinity.

Synthesis of Oligo(3-hydroxybutanoate)(OHB)-Containing Peptides with High Binding Affinity to a Class-I-MHC Protein

1998 ◽  
Vol 81 (5-8) ◽  
pp. 1181-1200 ◽  
Author(s):  
Dieter Seebach ◽  
Sorana Poenaru ◽  
Gerd Folkers ◽  
Didier Rognan
Keyword(s):  
Binding Affinity ◽  
Class I ◽  
Class I Mhc ◽  
High Binding ◽  
High Binding Affinity
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