Metabolic Engineering of a Methylmalonyl-CoA Mutase−Epimerase Pathway for Complex Polyketide Biosynthesis inEscherichia coli†,‡

Biochemistry ◽  
2002 ◽  
Vol 41 (16) ◽  
pp. 5193-5201 ◽  
Author(s):  
Linda C. Dayem ◽  
John R. Carney ◽  
Daniel V. Santi ◽  
Blaine A. Pfeifer ◽  
Chaitan Khosla ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli ◽  
Polyketide Biosynthesis

Metabolic engineering and transhydrogenase effects on NADPH availability inescherichia coli

2013 ◽  
Vol 29 (5) ◽  
pp. 1124-1130 ◽  
Author(s):  
Joanna Jan ◽  
Irene Martinez ◽  
Yipeng Wang ◽  
George N. Bennett ◽  
Ka-Yiu San
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli

Metabolic engineering strategies for D-lactate over production inEscherichia coli

2015 ◽  
Vol 91 (3) ◽  
pp. 576-584 ◽  
Author(s):  
Li Zhou ◽  
Wen-jing Cui ◽  
Zhong-mei Liu ◽  
Zhe-min Zhou
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli

Metabolic engineering of 2-pentanone synthesis inEscherichia coli

AIChE Journal ◽  
2013 ◽  
Vol 59 (9) ◽  
pp. 3167-3175 ◽  
Author(s):  
Ethan I. Lan ◽  
Yasumasa Dekishima ◽  
Derrick S. Chuang ◽  
James C. Liao
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli

scholarly journals Enhancement of Carrimycin Production Via Traditional Mutagenesis with Metabolic Engineering in Streptomyces Spiramyceticus 54IA

2022 ◽  
Author(s):  
Kemeng Li ◽  
Jianlu Dai ◽  
Juanjuan Liu ◽  
Tianyi Hao ◽  
Weiqing He
Keyword(s):  
Metabolic Engineering ◽  
Large Scale ◽  
Regulatory Gene ◽  
The Novel ◽  
Mutant Selection ◽  
Polyketide Biosynthesis ◽  
Primary Metabolites ◽  
Synthetic Genes ◽  
Differential Expression Genes ◽  
Selection Of

Abstract Background: Carrimycin is a new approved class I antibiotic in China. The novel carrimycin producing strain, Streptomyces spiramyceticus 54IA, was constructed by CRISPR-Cas9 editing system without insertion of antibiotics resistant gene. The problem of low yield limits this strain in large scale fermentation. In this study, the carrimycin production was significantly improved by strain mutagenesis coupled metabolic engineering. Results: The sspD gene is responsible for degradation of triacylglycerol to provide precursors of the polyketide biosynthesis. The extra sspD gene controlled by the promoters of pks and bsm42 genes could moderately enhance carrimycin production. The Bsm42 was identified to play a pathway-specific positive regulator for carrimycin biosynthesis. Due to production of carrimycin significantly enhanced by bsm42 overexpression, the two different length promoters of bsm42 individually ligated with two reporter genes were used to monitor bsm42 expression for screening the higher carrimycin production mutants treated by plasma and ultraviolet. 47% of the 608 selected mutants had higher fermentation titer than the starting strain. The shorter promoter of bsm42 displayed more appropriate for selection of the carrimycin production improved mutants. The F2R-15 mutant had highest titer (1010±30 μg/mL), which was about 9 times higher than that of 54IA strain. Comparative analysis of transcriptome profiles of F2R-15 mutant and 54IA strains found 158 differential expression genes with more than 2 fold-changes. The up-regulated genes were associated with macrolide precursor biosynthesis, macrolide-inactivation, antibiotics transporter, oxidative phosphorylation; while the most down-regulated genes were referring to the primary metabolites synthetic genes and biosynthetic genes of other secondary metabolites. Conclusion: These results suggested that manipulation of the positive regulatory gene bsm42 and traditional mutagenesis coupled with reporter-guided mutant selection method facilitated selection of carrimycin high-yielding mutants.

Biosynthesis of chain-specific alkanes by metabolic engineering inEscherichia coli

2015 ◽  
Vol 16 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Hong Yan ◽  
Zheng Wang ◽  
Fang Wang ◽  
Tianwei Tan ◽  
Luo Liu
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli

Metabolic engineering and protein directed evolution increase the yield ofL-phenylalanine synthesized from glucose inEscherichia coli

2004 ◽  
Vol 87 (4) ◽  
pp. 516-524 ◽  
Author(s):  
José Luis Báez-Viveros ◽  
Joel Osuna ◽  
Georgina Hernández-Chávez ◽  
Xavier Soberón ◽  
Francisco Bolívar ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Directed Evolution ◽  
Inescherichia Coli

scholarly journals Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore inEscherichia coli

FEBS Letters ◽  
2006 ◽  
Vol 580 (5) ◽  
pp. 1333-1338 ◽  
Author(s):  
Keiko Mukougawa ◽  
Hirosuke Kanamoto ◽  
Toshikazu Kobayashi ◽  
Akiho Yokota ◽  
Takayuki Kohchi
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli

scholarly journals Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering inEscherichia coli

2019 ◽  
Vol 14 (9) ◽  
pp. 1800438 ◽  
Author(s):  
Simon Boecker ◽  
Ahmed Zahoor ◽  
Thorben Schramm ◽  
Hannes Link ◽  
Steffen Klamt
Keyword(s):  
Metabolic Engineering ◽  
Inescherichia Coli
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