Soybean Lipoxygenase-1 Oxygenates Synthetic Polyenoic Fatty Acids with an Altered Positional Specificity. Evidence for Inverse Substrate Alignment†

Biochemistry ◽  
2001 ◽  
Vol 40 (34) ◽  
pp. 10223-10229 ◽  
Author(s):  
Igor Ivanov ◽  
Jörg Rathmann ◽  
Galina Myagkova ◽  
Hartmut Kuhn
Keyword(s):  
Fatty Acids ◽  
Soybean Lipoxygenase ◽  
Positional Specificity ◽  
Inverse Substrate

Trans Fatty Acids: Transport and Positional Specificity in Rat Placental Lecithin

Neonatology ◽  
1982 ◽  
Vol 42 (3-4) ◽  
pp. 138-144 ◽  
Author(s):  
C.E. Moore ◽  
G.A. Dhopeshwarkar
Keyword(s):  
Fatty Acids ◽  
Positional Specificity

Comparison of the Substrate Specificities of Lipoxygenases Purified from Soybean Seed, Wheat Seed, and Cucumber Cotyledons

1992 ◽  
Vol 47 (1-2) ◽  
pp. 85-89 ◽  
Author(s):  
Kenji Matsui ◽  
Hiroyuki Shinta ◽  
Hiromitsu Toyota ◽  
Tadahiko Kajiwara ◽  
Akikazu Hatanaka
Keyword(s):  
High Performance ◽  
Essential Role ◽  
Soybean Seed ◽  
Soybean Lipoxygenase ◽  
Carboxyl Group ◽  
Wheat Seed ◽  
Positional Specificity ◽  
Substrate Specificities ◽  
Substrate Analogues ◽  
Cucumber Cotyledons

Abstract Lipoxygenases were highly purified from soybean seed, wheat seed and cucumber cotyledons. Substrate specificities of these lipoxygenases were studied by using an entire series of (ω6Z ,ω9Z )-C13~C24-dienoic acids as synthetic substrate analogues. Soybean lipoxygenase-1 and cucumber lipoxygenase showed broad specificities for these substrates while wheat lipoxygenase showed narrow specificities. Position of dioxygenation to each substrate was analyzed by high performance liquid chromatography. With soybean lipoxygenase-1 elongation of the distance between the terminal carboxyl group and the site of hydrogen removal in a substrate decreased the positional specificity of dioxygenation, while, with cucumber lipoxygenase, shortening the distance decreased the specificity. It was suggested that cucumber lipoxygenase and soybean lipoxygenase-1 recognized the terminal carboxyl group of a substrate to arrange it only in one orientation at the reaction center. In case of wheat lipoxygenase, recognition of the carboxyl group was thought to have crucial and essential role to secure the activity.

Factors influencing the positional specificity of soybean lipoxygenase

1972 ◽  
Vol 289 (1) ◽  
pp. 82-87 ◽  
Author(s):  
John P. Christopher ◽  
Elfriede K. Pistorius ◽  
Fred E. Regnier ◽  
Bernard Axelrod
Keyword(s):  
Soybean Lipoxygenase ◽  
Positional Specificity ◽  
Factors Influencing

Oxygenation of Monounsaturated Fatty Acids by Soybean Lipoxygenase-1:  Evidence for Transient Hydroperoxide Formation†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15884-15892 ◽  
Author(s):  
Charles H. Clapp ◽  
Matthew Strulson ◽  
Pamela C. Rodriguez ◽  
Richmond Lo ◽  
Mark J. Novak
Keyword(s):  
Fatty Acids ◽  
Monounsaturated Fatty Acids ◽  
Soybean Lipoxygenase ◽  
Hydroperoxide Formation
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