Kinetic Analysis of Nonphotochemical Quenching of Chlorophyll Fluorescence. 2. Isolated Light-Harvesting Complexes†

Biochemistry ◽  
2001 ◽  
Vol 40 (33) ◽  
pp. 9902-9908 ◽  
Author(s):  
Mark Wentworth ◽  
Alexander V. Ruban ◽  
Peter Horton
Keyword(s):  
Chlorophyll Fluorescence ◽  
Kinetic Analysis ◽  
Light Harvesting ◽  
Nonphotochemical Quenching ◽  
Light Harvesting Complexes

scholarly journals A different perspective for nonphotochemical quenching in plant antenna complexes

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Edoardo Cignoni ◽  
Margherita Lapillo ◽  
Lorenzo Cupellini ◽  
Silvia Acosta-Gutiérrez ◽  
Francesco Luigi Gervasio ◽  
...  
Keyword(s):  
Light Harvesting ◽  
Nonphotochemical Quenching ◽  
Dual Function ◽  
Conformational Ensemble ◽  
Coulomb Interactions ◽  
Enhanced Sampling ◽  
Excitation Energies ◽  
Light Harvesting Complexes ◽  
Quenching Mechanisms ◽  
A Minor

AbstractLight-harvesting complexes of plants exert a dual function of light-harvesting (LH) and photoprotection through processes collectively called nonphotochemical quenching (NPQ). While LH processes are relatively well characterized, those involved in NPQ are less understood. Here, we characterize the quenching mechanisms of CP29, a minor LHC of plants, through the integration of two complementary enhanced-sampling techniques, dimensionality reduction schemes, electronic calculations and the analysis of cryo-EM data in the light of the predicted conformational ensemble. Our study reveals that the switch between LH and quenching state is more complex than previously thought. Several conformations of the lumenal side of the protein occur and differently affect the pigments’ relative geometries and interactions. Moreover, we show that a quenching mechanism localized on a single chlorophyll-carotenoid pair is not sufficient but many chlorophylls are simultaneously involved. In such a diffuse mechanism, short-range interactions between each carotenoid and different chlorophylls combined with a protein-mediated tuning of the carotenoid excitation energies have to be considered in addition to the commonly suggested Coulomb interactions.

scholarly journals A New Perspective for Nonphotochemical Quenching in Plant Antenna Complexes

2021 ◽  
Author(s):  
Edoardo Cignoni ◽  
Margherita Lapillo ◽  
Lorenzo Cupellini ◽  
Silvia Acosta Gutierrez ◽  
Francesco Luigi Gervasio ◽  
...  
Keyword(s):  
Light Harvesting ◽  
Nonphotochemical Quenching ◽  
Dual Function ◽  
Conformational Ensemble ◽  
Single Pair ◽  
Coulomb Interactions ◽  
Excitation Energies ◽  
Light Harvesting Complexes ◽  
New Perspective ◽  
A Minor

<div>Light-harvesting complexes (LHCs) of plants exert a dual function of light-harvesting and photoprotection. While LH processes are relatively well characterized, those involved in photoprotection are less understood. The main mechanism involved in photoprotection is to dissipate the energy absorbed by chlorophylls into harmless heat through processes collectively called nonphotochemical quenching (NPQ). Here, we characterize the quenching mechanisms of CP29, a minor LHC of plants with an important role in photoprotection, through two complementary enhanced-sampling techniques, dimensionality reduction schemes, electronic calculations and the analysis of cryo-EM data in the light of the predicted conformational ensemble. Our analysis reveals that the mechanism is more complex than previously thought. Several conformations of the lumenal side of the protein occur and differently affect the pigments relative geometries and interactions. Moreover, we show that a quenching mechanism localized on a single pair of pigments is not sufficient but many pigments are simultaneously involved. In such a diffuse mechanism, short-range interactions between each carotenoid and different chlorophylls combined with a protein-mediated tuning of the carotenoid excitation energies, have to be considered in addition to the commonly suggested coulomb interactions.</div>

scholarly journals A New Perspective for Nonphotochemical Quenching in Plant Antenna Complexes

2021 ◽  
Author(s):  
Edoardo Cignoni ◽  
Margherita Lapillo ◽  
Lorenzo Cupellini ◽  
Silvia Acosta Gutierrez ◽  
Francesco Luigi Gervasio ◽  
...  
Keyword(s):  
Light Harvesting ◽  
Nonphotochemical Quenching ◽  
Dual Function ◽  
Conformational Ensemble ◽  
Single Pair ◽  
Coulomb Interactions ◽  
Excitation Energies ◽  
Light Harvesting Complexes ◽  
New Perspective ◽  
A Minor

<div>Light-harvesting complexes (LHCs) of plants exert a dual function of light-harvesting and photoprotection. While LH processes are relatively well characterized, those involved in photoprotection are less understood. The main mechanism involved in photoprotection is to dissipate the energy absorbed by chlorophylls into harmless heat through processes collectively called nonphotochemical quenching (NPQ). Here, we characterize the quenching mechanisms of CP29, a minor LHC of plants with an important role in photoprotection, through two complementary enhanced-sampling techniques, dimensionality reduction schemes, electronic calculations and the analysis of cryo-EM data in the light of the predicted conformational ensemble. Our analysis reveals that the mechanism is more complex than previously thought. Several conformations of the lumenal side of the protein occur and differently affect the pigments relative geometries and interactions. Moreover, we show that a quenching mechanism localized on a single pair of pigments is not sufficient but many pigments are simultaneously involved. In such a diffuse mechanism, short-range interactions between each carotenoid and different chlorophylls combined with a protein-mediated tuning of the carotenoid excitation energies, have to be considered in addition to the commonly suggested coulomb interactions.</div>

Energy Transfer Pathways in Light-Harvesting Complexes of Purple Bacteria as Revealed by Global Kinetic Analysis of Two-Dimensional Transient Spectra

2013 ◽  
Vol 117 (38) ◽  
pp. 11349-11362 ◽  
Author(s):  
Evgeny E. Ostroumov ◽  
Rachel M. Mulvaney ◽  
Jessica M. Anna ◽  
Richard J. Cogdell ◽  
Gregory D. Scholes
Keyword(s):  
Energy Transfer ◽  
Kinetic Analysis ◽  
Light Harvesting ◽  
Purple Bacteria ◽  
Two Dimensional ◽  
Light Harvesting Complexes ◽  
Transient Spectra

scholarly journals Noise-Assisted Discord-Like Correlations in Light-Harvesting Photosynthetic Complexes

2021 ◽  
Vol 3 (2) ◽  
pp. 262-271
Author(s):  
Pablo Reséndiz-Vázquez ◽  
Ricardo Román-Ancheyta ◽  
Roberto León-Montiel
Keyword(s):  
Potential Role ◽  
Energy Transport ◽  
Light Harvesting ◽  
Quantum Correlations ◽  
Transport Processes ◽  
Quantum Evolution ◽  
Light Harvesting Complexes ◽  
Transport Efficiency ◽  
Photovoltaic Materials ◽  
Photosynthetic Complexes

Transport phenomena in photosynthetic systems have attracted a great deal of attention due to their potential role in devising novel photovoltaic materials. In particular, energy transport in light-harvesting complexes is considered quite efficient due to the balance between coherent quantum evolution and decoherence, a phenomenon coined Environment-Assisted Quantum Transport (ENAQT). Although this effect has been extensively studied, its behavior is typically described in terms of the decoherence’s strength, namely weak, moderate or strong. Here, we study the ENAQT in terms of quantum correlations that go beyond entanglement. Using a subsystem of the Fenna–Matthews–Olson complex, we find that discord-like correlations maximize when the subsystem’s transport efficiency increases, while the entanglement between sites vanishes. Our results suggest that quantum discord is a manifestation of the ENAQT and highlight the importance of beyond-entanglement correlations in photosynthetic energy transport processes.

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