Determination of Amino Acid Sequences in Oligopeptides by Mass Spectrometry. IV. Synthetic N-Acyl Oligopeptide Methyl Esters*

Biochemistry ◽  
1965 ◽  
Vol 4 (10) ◽  
pp. 2254-2260 ◽  
Author(s):  
E. Bricas ◽  
J. van Heijenoort ◽  
M. Barber ◽  
W. A. Wolstenholme ◽  
B. C. Das ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Methyl Esters ◽  
Amino Acid Sequences

Mass spectrometric studies of peptides. V. Determination of amino acid sequences in peptide mixtures by mass spectrometry

1973 ◽  
Vol 95 (10) ◽  
pp. 3369-3375 ◽  
Author(s):  
Hans Kaspar. Wipf ◽  
Philip. Irving ◽  
Malcolm. McCamish ◽  
Rengachari. Venkataraghavan ◽  
F. W. McLafferty
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Mass Spectrometric ◽  
Amino Acid Sequences ◽  
Peptide Mixtures

scholarly journals Determination of amino acid sequences in peptides by mass spectrometry. Desulfurization of sulfur-containing peptides

FEBS Letters ◽  
1970 ◽  
Vol 8 (4) ◽  
pp. 207-209 ◽  
Author(s):  
R. Toubiana ◽  
J.E.G. Barnett ◽  
E. Sach ◽  
B.C. Das ◽  
E. Lederer
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences ◽  
Sulfur Containing

Determination of amino acid sequences in oligopeptides by mass spectrometry. II. The structure of peptidolipin NA

1965 ◽  
Vol 6 (18) ◽  
pp. 1331-1336 ◽  
Author(s):  
M. Barber ◽  
W.A. Wolstenholme ◽  
M. Guinand ◽  
G. Michel ◽  
B.C. Das ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Amino Acid Sequences

Resolution of a protein sequence ambiguity by X-ray crystallographic and mass spectrometric methods

1992 ◽  
Vol 25 (2) ◽  
pp. 205-210 ◽  
Author(s):  
L. J. Keefe ◽  
E. E. Lattman ◽  
C. Wolkow ◽  
A. Woods ◽  
M. Chevrier ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acid ◽  
Electron Density ◽  
Mass Spectrometric ◽  
Amino Acid Sequences ◽  
Data Matrix ◽  
Protein Crystal ◽  
Insertion Mutant ◽  
Laser Desorption Mass Spectrometry ◽  
X Ray

Ambiguities in amino acid sequences are a potential problem in X-ray crystallographic studies of proteins. Amino acid side chains often cannot be reliably identified from the electron density. Many protein crystal structures that are now being solved are simple variants of a known wild-type structure. Thus, cloning artifacts or other untoward events can readily lead to cases in which the proposed sequence is not correct. An example is presented showing that mass spectrometry provides an excellent tool for analyzing suspected errors. The X-ray crystal structure of an insertion mutant of Staphylococcal nuclease has been solved to 1.67 Å resolution and refined to a crystallographic R value of 0.170 [Keefe & Lattman (1992). In preparation]. A single residue has been inserted in the C-terminal α helix. The inserted amino acid was believed to be an alanine residue, but the final electron density maps strongly indicated that a glycine had been inserted instead. To confirm the observations from the X-ray data, matrix-assisted laser desorption mass spectrometry was employed to verify the glycine insertion. This mass spectrometric technique has sufficient mass accuracy to detect the methyl group that distinguishes glycine from alanine and can be extended to the more common situation in which crystallographic measurements suggest a problem with the sequence, but cannot pinpoint its location or nature.

scholarly journals Determination of free amino acids in plants by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)

2015 ◽  
Vol 7 (18) ◽  
pp. 7574-7581 ◽  
Author(s):  
Magdalena M. Dziągwa-Becker ◽  
Jose M. Marin Ramos ◽  
Jakub K. Topolski ◽  
Wiesław A. Oleszek
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Tandem Mass Spectrometry ◽  
Free Amino ◽  
Tandem Mass ◽  
Amino Acid Determination ◽  
Acid Determination

Free amino acid determination in plants by LC-MS/MS.

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