Comparative Studies of Catalytic Properties of Guinea Pig Liver Intra- and Extramitochondrial Phosphoenolpyruvate Carboxykinases*

Biochemistry ◽  
1965 ◽  
Vol 4 (4) ◽  
pp. 723-731 ◽  
Author(s):  
Darold D. Holten ◽  
Robert C. Nordlie
Keyword(s):  
Guinea Pig ◽  
Comparative Studies ◽  
Catalytic Properties ◽  
Pig Liver ◽  
Guinea Pig Liver

scholarly journals Biliary excretion of some anionic derivatives of diethylstilboestrol and phenolphthalein in the guinea pig

1977 ◽  
Vol 168 (3) ◽  
pp. 373-377 ◽  
Author(s):  
P A Barford ◽  
A H Olavesen ◽  
C G Curtis ◽  
G M Powell
Keyword(s):  
Guinea Pig ◽  
Biliary Excretion ◽  
Comparative Studies ◽  
Enzyme Activities ◽  
Species Differences ◽  
Pig Liver ◽  
Biliary Elimination ◽  
Derivatives Of ◽  
Guinea Pig Liver

The metabolic fates and modes of excretion of diethylstilboestrol mono[35S]sulphate and diethylstilboestrol di[35S]sulphate were studied in the guinea pig. Comparative studies were also made with [G-3H]diethylstilboestrol and phenolphthalein di[35S]sulphate. Diethylstiboesterol di[35S]sulphate was extensively eliminated in the bile unchanged. After administration of diethylstilboestrol mono[35S]sulphate, extensive biliary elimination of radioactivity was also recorded. Radioactive components were identified as diethylstilboestrol disulphate, diethylstilboestrol monosulphate monoglucuronide and unchanged diethylstilboestrol monosulphate. When [G-3H]diethylstilboestrol was administered, 3H-labelled diethylstilboestrol monoglucuronide, diethylstilboestrol monosulphate monoglucuronide and diethylstilboestrol disulphate appeared in the bile. Phenolphthalein di[35S]sulphate was excreted unchanged in bile. These findings are discussed in relation to studies carried out in the rat [Barford, Olavesen, Curtis & Powell (1977) Biochem. J. 164, 423–430] and species differences are related to differences in enzyme activities in rat and guinea-pig liver.

Die Bedeutung der Homogenisationsart und -intensität für die Größe und Konstanz der Sauerstoffaufnahme von Meerschweinchen-Leberhomogenat / The Influence of Mode and Intensity of Homogenization on the Absolute Value and Stability of Oxygen Consumption of Guinea Pig Liver Homogenates

1977 ◽  
Vol 32 (11-12) ◽  
pp. 908-912 ◽  
Author(s):  
H. J. Schmidt ◽  
U. Schaum ◽  
J. P. Pichotka
Keyword(s):  
Oxygen Uptake ◽  
Guinea Pig ◽  
Measuring Time ◽  
Pig Liver ◽  
Absolute Value ◽  
Modified Method ◽  
Simultaneous Measurements ◽  
Liver Homogenates ◽  
The Absolute ◽  
Guinea Pig Liver

Abstract The influence of five different methods of homogenisation (1. The method according to Potter and Elvehjem, 2. A modification of this method called Potter S, 3. The method of Dounce, 4. Homogenisation by hypersonic waves and 5. Coarce-grained homogenisation with the “Mikro-fleischwolf”) on the absolute value and stability of oxygen uptake of guinea pig liver homogenates has been investigated in simultaneous measurements. All homogenates showed a characteristic fall of oxygen uptake during measuring time (3 hours). The modified method according to Potter and Elvehjem called Potter S showed reproducible results without any influence by homogenisation intensity.

Precision-cut Guinea-pig Liver Slices as a Tool for Studying the Toxicity of Volatile Anaesthetics

1990 ◽  
Vol 18 (1_part_1) ◽  
pp. 191-199
Author(s):  
Hanan N. Ghantous ◽  
Jeanne Fernando ◽  
Scott E. Morgan ◽  
A. Jay Gandolfi ◽  
Klaus Brandel
Keyword(s):  
Guinea Pig ◽  
Rank Order ◽  
Normal Liver ◽  
Liver Slice ◽  
Volatile Anaesthetics ◽  
Pig Liver ◽  
Liver Slices ◽  
Guinea Pig Liver ◽  
Krebs Henseleit Buffer

Cultured precision-cut liver slices retain normal liver architecture and physiological biochemical functions. Hartley male guinea-pig liver slices have proven to be a good model for studying the biotransformation and toxicity of halothane. This system was used to evaluate the biotransformation and toxicity of different volatile anaesthetics (halothane, enflurane, isoflurane and sevoflurane), and compare their effects to those of new anaesthetics (desflurane). Liver slices (250–300μm thick) were incubated in sealed roller vials, containing Krebs Henseleit buffer at 37°C under 95% O2:5% CO2 atmosphere. Volatile anaesthetics were delivered by volatilisation after pre-incubation for 1 hour to produce a constant concentration in the medium. Production of the metabolites, trifluroacetic acid and fluoride ion, was measured. Intracellular potassium ion content, protein synthesis and secretion were determined as indicators of viability of the slices. The rank order of biotransformation of anaesthetics by the liver slices was halothane >sevoflurane>isoflurane and enflurane>desflurane. The rank order of hepatotoxicity of these anaesthetics was halothane>isoflurane and enflurane>sevoflurane and desflurane. Halothane is the anaesthetic which is metabolised furthest and has the most toxic effect, while desflurane is the least metabolised anaesthetic and has the least toxicity. This in vitro cultured precision-cut liver slice system appears to be suitable for studying the biotransformation of volatile anaesthetics and correlating its role in the resulting toxicity.

scholarly journals Exolytic hydrolysis of toxic plant glucosides by guinea pig liver cytosolic beta-glucosidase.

1992 ◽  
Vol 267 (20) ◽  
pp. 14027-14032
Author(s):  
V Gopalan ◽  
A Pastuszyn ◽  
W R Galey ◽  
R.H. Glew
Keyword(s):  
Guinea Pig ◽  
Pig Liver ◽  
Beta Glucosidase ◽  
Toxic Plant ◽  
Hydrolysis Of ◽  
Guinea Pig Liver

scholarly journals THE REDUCTION OF l-XYLULOSE TO XYLITOL BY GUINEA PIG LIVER MITOCHONDRIA

1956 ◽  
Vol 221 (2) ◽  
pp. 697-709 ◽  
Author(s):  
Oscar Touster ◽  
V.H. Reynolds ◽  
Ruth M. Hutcheson
Keyword(s):  
Guinea Pig ◽  
Liver Mitochondria ◽  
Pig Liver ◽  
Guinea Pig Liver
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