Sterol Biosynthesis in the Bovine Corpus Luteum in Vitro*

Biochemistry ◽  
1966 ◽  
Vol 5 (9) ◽  
pp. 2944-2956 ◽  
Author(s):  
Hermione R. Hellig ◽  
Kenneth Savard
Keyword(s):  
Corpus Luteum ◽  
Sterol Biosynthesis ◽  
Bovine Corpus Luteum

scholarly journals Control of Steroidogenesis in Small and Large Bovine Luteal. Cells

1987 ◽  
Vol 40 (3) ◽  
pp. 331 ◽  
Author(s):  
William Hansel ◽  
Hector W Alila ◽  
Joseph P Dowd ◽  
Xiangzhong Yang
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Corpus Luteum ◽  
Luteal Cell ◽  
Lipoxygenase Pathway ◽  
Luteal Cells ◽  
Kinase C ◽  
Bovine Corpus Luteum ◽  
Progesterone Synthesis

Evidence was cited to show that: (1) prostacyclin (PGI2) plays a luteotrophic role in the bovine corpus luteum and that products of the lipoxygenase pathway of arachidonic acid metabolism, especially 5-hydroxyeicosatetraenoic acid play luteolytic roles; (2) oxytocin of luteal cell origin plays a role in development, and possibly in regression, of the bovine corpus luteum; and (3) luteal cells arise from two sources; the characteristic small luteal cells at all stages of the o~strous cycle and pregnancy are of theca cell origin; the large cells are of granulosa cell origin early in the cycle, but a population of theca-derived large cells appears later in the cycle. Results of in vitro studies with total dispersed cells and essentially pure preparations of large and small luteal cells indicate that : (1) the recently described Ca2+ -polyphosphoinositol-protein kinase C second messenger system is involved in progesterone synthesis in the bovine corpus luteum; (2) activation of protein kinase C is stimulatory to progesterone synthesis in the small luteal cells; (3) activation of protein kinase C has no effect on progesterone synthesis in the large luteal cells; and (4) protein kinase C exerts its luteotrophic effect in total cell preparations, in part at least, by stimulating the production of prostacyclin. The protein kinase C system may cause down regulation of LH receptors in the large cells.

scholarly journals Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy

Reproduction ◽  
2015 ◽  
Vol 150 (3) ◽  
pp. 217-225 ◽  
Author(s):  
Koumei Shirasuna ◽  
Haruka Matsumoto ◽  
Shuichi Matsuyama ◽  
Koji Kimura ◽  
Heinrich Bollwein ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Neutrophil Migration ◽  
Peripheral Circulation ◽  
Interferon Tau ◽  
Bovine Corpus Luteum ◽  
Activated Neutrophils ◽  
Endocrine Factor ◽  
Uterine Environment ◽  
And Function

When pregnancy is established, interferon tau (IFNT), a well-known pregnancy recognition signal in ruminants, is secreted by embryonic trophoblast cells and acts within the uterus to prepare for pregnancy. IFNT acts as an endocrine factor on the corpus luteum (CL) to induce refractory ability against the luteolytic action of PGF2α. Hypothesising that IFNT may influence not only the uterine environment but also the CL in cows via local or peripheral circulation, we investigated qualitative changes in the CL of pregnant cows during the maternal recognition period (day 16) and the CL of non-pregnant cows. The CL of pregnant animals had a higher number of neutrophils, and the expression of interleukin 8 (IL8) mRNA and its protein was higher as well as compared with the CL of non-pregnant animals. Although IFNT did not affect progesterone (P4) secretion and neutrophil migration directly, it stimulated IL8 mRNA expression on luteal cells (LCs), influencing the neutrophils, resulting in the increased migration of IFNT-activated neutrophils. Moreover, both IFNT-activated neutrophils and IL8 increased P4 secretion from LCs in vitro. Our novel finding was the increase in neutrophils and IL8 within the CL of pregnant cows, suggesting the involvement of IFNT function within the CL toward establishment of pregnancy in cows. The present results suggest that IFNT upregulates neutrophil numbers and function via IL8 on LCs in the CL of early pregnant cows and that both neutrophils and IL8, stimulated by IFNT, are associated with an increase in P4 concentrations during the maternal recognition period in cows.

66 Bovine corpus luteum affects embryo developmental competence and production

2019 ◽  
Vol 31 (1) ◽  
pp. 158
Author(s):  
J. M. Alvarado ◽  
M. A. Tenemaza ◽  
S. L. Merchán ◽  
D. E. Argudo ◽  
M. S. Méndez ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Developmental Competence ◽  
Average Weight ◽  
Embryo Production ◽  
Fluid Medium ◽  
Bovine Corpus Luteum ◽  
Developmental Capacity ◽  
Recorded Data ◽  
Fetal Bovine

This study was conducted with the aim to determine the effect of the bovine corpus luteum on in vitro embryo production. Immature cumulus-oocyte complexes (COC) were aspirated from abattoir ovaries from cows (mainly Holstein and dairy crossbred cows and heifers) with (ipsilateral; CL+) and without (contralateral; CL−) corpus luteum (CL), and from cows without CL in any of the ovaries. The average weight of the ovaries from each group was 10.4±0.25, 5.7±0.25, and 6.1±0.25g for CL+, CL−, and C, respectively. The experiment was completed within 12 replicates (100 ovaries per group). The COC were cultured in maturation medium (TCM-199 supplemented with 10% fetal bovine serum, 100µg mL−1 sodium pyruvate, 0.75mg mL−1 of L-glutamine, 4µg mL−1 of FSH-p, 100 µM cysteamine, and 250µg mL−1 of gentamicin) followed by IVF (synthetic oviducal fluid medium supplemented with 10µg mL−1 heparin) and in vitro culture (citrate synthetic oviducal fluid medium). On Day 7 after IVF, the embryos were evaluated and classified into morulae (M), early blastocysts (EB), regular blastocysts (RB), expanded blastocysts (ExB), and hatched blastocysts (HB), and the embryos with quality 1 (according to IETS criteria) were recorded. Data were analysed by logistic regression and general linear model of SAS (SAS Institute Inc., Cary, NC, USA), and means were compared by the least squares means method. Results of cleavage, embryo rate at Day 7, and rates of M+EB, RB, ExB, and HB are shown in Table 1. The number of embryos per ovary was greater (P<0.01) in CL+ (1.16±0.11) than in CL− (0.45±0.15) and C (0.55±0.15). Also, the number of embryos per cultured oocyte was significantly greater in CL+ than in CL− and C (0.27±0.02v. 0.14±0.03 and 0.15±0.03, respectively; P<0.01). The results of this study reveal that the presence of the corpus luteum in the ovary at the time of the oocyte recovery affects the developmental capacity of the bovine embryos, and such influence probably occur through intraovarian interactions. Table 1.Effect of bovine corpus luteum on embryo production

In vitro inhibition of cholesterol synthesis by pregnenolone in bovine corpus luteum

Steroids ◽  
1967 ◽  
Vol 9 (4) ◽  
pp. 405-414 ◽  
Author(s):  
Ajai Haksar ◽  
E.B. Romanoff ◽  
N. Hagino ◽  
G. Pincus
Keyword(s):  
Corpus Luteum ◽  
Cholesterol Synthesis ◽  
Bovine Corpus Luteum ◽  
In Vitro Inhibition

IN VITRO ACETATE-l-C14 METABOLISM BY THE BOVINE CORPUS LUTEUM (C.L.)

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S95 ◽  
Author(s):  
A. Oriol-Bosch ◽  
E. B. Romanoff
Keyword(s):  
Corpus Luteum ◽  
Bovine Corpus Luteum
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