The Effect of Hydrogen Peroxide on Glucose Oxidase from Aspergillus niger*

Biochemistry ◽  
1966 ◽  
Vol 5 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Kjell Kleppe
Keyword(s):  
Hydrogen Peroxide ◽  
Aspergillus Niger ◽  
Glucose Oxidase

scholarly journals Expression of Aspergillus niger glucose oxidase in Pichia pastoris and its antimicrobial activity against Agrobacterium and Escherichia coli

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9010
Author(s):  
Yonggang Wang ◽  
Jiangqin Wang ◽  
Feifan Leng ◽  
Jianzhong Ma ◽  
Alnoor Bagadi
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Aspergillus Niger ◽  
Pichia Pastoris ◽  
Glucose Oxidase ◽  
Fermentation Medium ◽  
Logarithmic Phase ◽  
Transgenic Strain ◽  
E Coli ◽  
Cell Lysate

The gene encoding glucose oxidase from Aspergillus niger ZM-8 was cloned and transferred to Pichia pastoris GS115, a transgenic strain P. pastoris GS115-His-GOD constructed. The growth curve of P. pastoris GS115-His-GOD was consistent with that of Pichia pastoris GS115-pPIC9K under non-induced culture conditions. Under methanol induction conditions, the growth of the GOD-transgenic strain was significantly lowered than P. pastoris GS115-pPIC9K with the induced-culture time increase, and the optical densities of GOD-transgenic strain reached one-third of that of the P. pastoris GS115-pPIC9K at 51 h. The activity of glucose oxidase in the cell-free supernatant, the supernatant of cell lysate, and the precipitation of cell lysate was 14.3 U/mL, 18.2 U/mL and 0.48 U/mL, respectively. The specific activity of glucose oxidase was 8.3 U/mg, 6.52 U/mg and 0.73 U/mg, respectively. The concentration of hydrogen peroxide formed by glucose oxidase from supernatant of the fermentation medium, the supernatant of the cell lysate, and the precipitation of cell lysate catalyzing 0.2 M glucose was 14.3 μg/mL, 18.2 μg/mL, 0.48 μg/mL, respectively. The combination of different concentrations of glucose oxidase and glucose could significantly inhibit the growth of Agrobacterium and Escherichia coli in logarithmic phase. The filter article containing supernatant of the fermentation medium, supernatant of the cell lysate, and precipitation of cell lysate had no inhibitory effect on Agrobacterium and E. coli. The minimum inhibitory concentration of hydrogen peroxide on the plate culture of Agrobacterium and E. coli was 5.6 × 103 μg/mL and 6.0 × 103 μg/mL, respectively.

Effects of metal ions on simultaneous production of glucose oxidase and catalase by Aspergillus niger

2001 ◽  
Vol 32 (1) ◽  
pp. 16-19 ◽  
Author(s):  
J.-Z. Liu ◽  
Y.-Y. Huang ◽  
J. Liu ◽  
L.-P. Weng ◽  
L.-N. Ji
Keyword(s):  
Aspergillus Niger ◽  
Metal Ions ◽  
Glucose Oxidase ◽  
Simultaneous Production

15N- AND 13C-NMR INVESTIGATIONS OF GLUCOSE OXIDASE FROM ASPERGILLUS NIGER

1991 ◽  
pp. 209-212
Author(s):  
Christoph Sanner ◽  
Peter Macheroux ◽  
Heinz Rüterjans ◽  
Franz Müller ◽  
Adalbert Bacher
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
13C Nmr

Diauxic production of glucose oxidase by Aspergillus niger in submerged culture

2002 ◽  
Vol 31 (5) ◽  
pp. 615-620 ◽  
Author(s):  
J. Mirón ◽  
M.P. González ◽  
L. Pastrana ◽  
M.A. Murado
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase ◽  
Submerged Culture

Induction of glucose oxidase, catalase, and lactonase in Aspergillus niger

1993 ◽  
Vol 24 (5) ◽  
pp. 408-416 ◽  
Author(s):  
Cor F. B. Witteveen ◽  
Peter J. I. van de Vondervoort ◽  
Hetty C. van den Broeck ◽  
Frank A. C. van Engelenburg ◽  
Leo H. de Graaff ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Glucose Oxidase

scholarly journals Enzymatically triggered rupture of polymersomes

Soft Matter ◽  
2016 ◽  
Vol 12 (4) ◽  
pp. 1014-1020 ◽  
Author(s):  
Woo-Sik Jang ◽  
Seung Chul Park ◽  
Ellen H. Reed ◽  
Kevin P. Dooley ◽  
Samuel F. Wheeler ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Glucose Oxidase

Polymersomes are robust vesicles made from di-block co-polymers. We have engineered a two step enzymatic cascade to trigger the release of contents from polymersomes, in which extravesicular glucose oxidase makes hydrogen peroxide, when then penetrates the membrane and is converted by entrapped catalase to oxygen, leading to vesicle failure.

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