In Vitro Protein Synthesis Directed by R17 Viral Ribonucleic Acid. I. Initiation of Polypeptide Synthesis in a Purified Cell-Free System*

Biochemistry ◽  
1967 ◽  
Vol 6 (8) ◽  
pp. 2571-2578 ◽  
Author(s):  
Satomi Igarashi ◽  
William Paranchych
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Free System ◽  
In Vitro Protein Synthesis ◽  
Cell Free System ◽  
Polypeptide Synthesis ◽  
Vitro Protein

Inhibition of in vitro protein synthesis in a rabbit reticulocyte cell-free system by toxic trypsic peptides from ricin

Biochimie ◽  
1974 ◽  
Vol 56 (9) ◽  
pp. 1287-1289 ◽  
Author(s):  
Alain A.J. Lugnier ◽  
Marie Anne Le Meur ◽  
Pierre Gerlinger ◽  
Guy Dirheimer
Keyword(s):  
Protein Synthesis ◽  
Free System ◽  
In Vitro Protein Synthesis ◽  
Cell Free System ◽  
Vitro Protein

In vitro protein synthesis directed by R17 viral ribonucleic acid. II. On the fate of mRNA in the cell-free system

1969 ◽  
Vol 47 (12) ◽  
pp. 1179-1186 ◽  
Author(s):  
Satomi J. Igarashi
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid Incorporation ◽  
Free System ◽  
Cell Free System ◽  
E Coli ◽  
Acid Incorporation ◽  
Binding Factor ◽  
Vitro Protein

In the crude E. coli B cell-free system, mRNA was hydrolyzed by contaminating nuclease activities before significant polymerization of amino acids took place. Ribosomes appeared to be one of the sources of nuclease. A modified high-salt washing procedure was developed to remove nuclease from ribosomes. RNase-free ribosomes thus obtained appeared to be inactive in poly-U-directed phenylalanine incorporation, unless poly-U binding factor was added to the system. R17 RNA could not direct amino acid incorporation in the presence of RNase-free ribosomes because binding of intact R17 RNA to ribosomes did not take place even in the presence of poly-U binding factor.

The effect of synthetic lysine and methionine on in vitro protein synthesis of weaning pigs

1995 ◽  
Vol 15 (3) ◽  
pp. 429-437 ◽  
Author(s):  
Y.M. Kwon ◽  
K.N. Heo ◽  
Y.J. Choi ◽  
I.K. Han ◽  
J.H. Woo
Keyword(s):  
Protein Synthesis ◽  
In Vitro Protein Synthesis ◽  
Weaning Pigs ◽  
Vitro Protein

[37] In vitro protein synthesis

1991 ◽  
pp. 536-545 ◽  
Author(s):  
Michael J. Leibowitz ◽  
Francis P. Barbone ◽  
Denise E. Georgopoulos
Keyword(s):  
Protein Synthesis ◽  
In Vitro Protein Synthesis ◽  
Vitro Protein
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