N6-(Δ2-Isopentenyl)adenosine. A Component of the Transfer Ribonucleic Acid of Yeast and of Mammalian Tissue, Methods of Isolation, and Characterization*

Biochemistry ◽  
1967 ◽  
Vol 6 (6) ◽  
pp. 1837-1848 ◽  
Author(s):  
Morris J. Robins ◽  
Ross H. Hall ◽  
Roosevelt Thedford
1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.


1962 ◽  
Vol 10 (1) ◽  
pp. 36-38 ◽  
Author(s):  
MARVIN MURRAY

A method for demonstrating nuclear ribonucleic acid is given. This entails the use of Grams' stain of heat fixed tissues. The selectivity of crystal violet for nuclear ribonuclei acid suggests a fundamental difference between the nuclear and cytoplasmic varieties of this substance.


Sign in / Sign up

Export Citation Format

Share Document