The Use of Computed Optical Rotatory Dispersion Curves for the Evaluation of Protein Conformation*

Biochemistry ◽  
1967 ◽  
Vol 6 (6) ◽  
pp. 1630-1637 ◽  
Author(s):  
Norma Greenfield ◽  
Betty Davidson ◽  
Gerald D. Fasman
Keyword(s):  
Protein Conformation ◽  
Dispersion Curves ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory

The effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase

1968 ◽  
Vol 46 (10) ◽  
pp. 1231-1235 ◽  
Author(s):  
William D. Ellis ◽  
H. Brian Dunford
Keyword(s):  
Horseradish Peroxidase ◽  
Ligand Binding ◽  
Protein Conformation ◽  
Spectral Region ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Helical Content ◽  
Protein Moiety ◽  
Soret Region

The optical rotatory dispersion of horseradish peroxidase and its cyanide, fluoride, and hydroxide complexes was studied in the spectral region 215–450 mμ, and that of the azide complex at 350–450 mμ. The effect that splitting the heme from the protein of peroxidase has on the optical rotatory dispersion in the 215–450 mμ region was also studied. Results of measurements of the reduced mean residue rotation at 233 mμ, lead to the conclusion that there are no significant changes in gross protein conformation upon the binding of ligands to peroxidase, but that the splitting of the heme causes a reduction of the helical content of the protein. Pure peroxidase was estimated to have 43% α-helical content, which was reduced to 33% when the heme was split from the protein. Results of studies in the Soret region indicate that the binding of various ligands does not cause an alteration of the geometry of the heme with respect to the protein moiety.

STÉROÏDES: CCXIX PREPARATION ET PROPRIÉTÉS DE QUELQUES 17-ISO-PREGNANES ET 17-ISO-PREGNÈNES SUBSTITUÉS EN C-16

1963 ◽  
Vol 41 (1) ◽  
pp. 156-164 ◽  
Author(s):  
Pierre Crabbé ◽  
Magdalena Pérez ◽  
Gloria Vera
Keyword(s):  
Dispersion Curves ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory

The preparation of 16β-carboxy-(5α)-17α-pregnane-3β-ol-20-one and 16β-carbomethoxy-3β-fluoro-17α-Δ5-pregnene-20-one, as well as several of their derivatives, is described. The stereochemistry of these compounds is deduced from their optical rotatory dispersion curves.

Sign in / Sign up

Export Citation Format

Share Document