Conformational and immunological properties of a bovine brain acidic protein (S-100)

Biochemistry ◽  
1968 ◽  
Vol 7 (2) ◽  
pp. 758-764 ◽  
Author(s):  
Dietrich. Kessler ◽  
Lawrence. Levine ◽  
Gerald D. Fasman
Keyword(s):  
Protein S ◽  
Bovine Brain ◽  
Acidic Protein ◽  
Immunological Properties ◽  
S 100

Demonstration of subunits in beef brain acidic protein (S-100)

1969 ◽  
Vol 37 (4) ◽  
pp. 587-592 ◽  
Author(s):  
Priscilla S. Danniest ◽  
Lawrence Levine
Keyword(s):  
Protein S ◽  
Acidic Protein ◽  
Beef Brain ◽  
S 100

scholarly journals Neurotrophic factors concentration in rat brain at the experimental coma

2013 ◽  
Vol 94 (5) ◽  
pp. 695-699 ◽  
Author(s):  
V A Kashuro ◽  
E G Batotsyrenova ◽  
N L Elaeva ◽  
U N Savenko ◽  
N V Lapina ◽  
...  
Keyword(s):  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Protein S ◽  
Serum Levels ◽  
Brain Derived Neurotrophic Factor ◽  
Acidic Protein ◽  
Ethanol Administration ◽  
S 100 ◽  
Protein Serum ◽  
Glial Fibrillar Acidic Protein

Aim. To study the changes of neurotrophic factors concentrations depending on stage of intoxication with deprivation substances (ethanol or sodium oxybutirate) in rats. Methods. Experiments were performed on male white laboratory rats. Control and experimental groups included 10 animals each. Half-lethal doses of a deprivation substance (ethanol or sodium oxybutirate) were introduced intraperitoneally 3, 6, 12, 24 and 72 hours before blood specimen collection. Neuron-specific enolase, S-100 protein, brain-derived neurotrophic factor, pigment epithelium-derived factor, glial fibrillary acidic protein serum levels were measured by enzyme immunoassay. Results. At single infusion of mean lethal dose of sodium oxybutyrate S-100 protein serum level significantly increased after 6 hours compared to control and stayed elevated during the first 24 hours. The levels of neurotrophic and neuroprotective factors also significantly increased 3 and 6 hours after the drug administration. The toxic dose of ethanol have significantly increased (over than 1.8 times compared to the controls) the concentration of protein S-100 in rats after 3 hours. The maximum increase in the protein S-100 level (by 2.6 times and over) was noted 12 hours after the toxicant administration. Glial fibrillar acidic protein concentration was 2.9 times higher compared to controls 3 hours after and 1.9 times higher 6 hours after higher the ethanol administration (р 0.05). The concentration of brain - derived neurotrophic factor has also increased from 3 to 12 hours after the toxicant administration, and was 2.1 to 2.4 times higher compared to intact animals. Conclusion. Studying of neurotrophic factors brain in plasma showed that the development of hypoxia, accompanying coma, leads to higher serum levels of S-100 protein, brain-derived neurotrophic factor and glial fibrillar acidic protein. The increase in the concentration of S-100 is a marker for the presence of brain damage. The observed increase of glial fibrillar acidic protein in experiments with ethanol may indicate its more severe brain tissue damage compared to sodium oxybutyrate.

scholarly journals Immunohistochemical Characterization of Canine Transmissible Venereal Tumor

1996 ◽  
Vol 33 (3) ◽  
pp. 257-263 ◽  
Author(s):  
E. Mozos ◽  
A. Méndez ◽  
J. C. Gómez-Villamandos ◽  
J. Martín de las Mulas ◽  
J. Pérez
Keyword(s):  
Light Chain ◽  
B Lymphocyte ◽  
Protein S ◽  
Lambda Light Chain ◽  
Formalin Fixed Paraffin ◽  
S 100 ◽  
Formalin Fixed Paraffin Embedded ◽  
Progressive Growth ◽  
Staining Techniques ◽  
Alpha 1 Antitrypsin

The collective immunohistochemical expression of human lysozyme, human alpha-1-antitrypsin, human CD3 antigen, calf vimentin, human keratins, human lambda light chains, canine immunoglobulins IgG, IgM, and bovine protein S-100 has been analyzed on formalin-fixed, paraffin-embedded tissue sections of 25 spontaneous canine transmissible venereal tumors (CTVT) from both genital and extragenital locations using the avidin-biotin-peroxidase complex technique. Lysozyme immunoreactivity was detected in 10/25 CTVT, alpha-1-antitrypsin in 14/25 CTVT, and vimentin in 25/25 CTVT. All CTVT cells were negative to keratins 5 + 8 of the Moll catalogue (RCK-102), S-100 protein, lambda light-chain immunoglobulins, IgG, IgM, and CD3 antigen. The intratumoral T- and B-lymphocyte infiltrate was differentiated using CD3 antigen, lambda light-chain immunoglobulins, IgG, and IgM, and this technique could be useful to evaluate the regressive or progressive growth stage of venereal tumors. Our findings support the hypothesis of a histiocytic immunophenotype for CTVT, and these staining techniques could be used in the differential diagnosis with lymphomas.

scholarly journals Locally advanced malignant solitary fibrous tumour successfully treated with conversion chemotherapy, operation and postoperative radiotherapy: a case report

2021 ◽  
Vol 49 (3) ◽  
pp. 030006052199694
Author(s):  
Zhi-Ke Li ◽  
Jie Liu ◽  
Chen Chen ◽  
Ke-Yi Yang ◽  
Yao-Tiao Deng ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Protein ◽  
Postoperative Radiotherapy ◽  
Locally Advanced ◽  
Rna Binding Protein ◽  
Disease Free Survival ◽  
Protein S ◽  
Solitary Fibrous Tumour ◽  
Final Pathology ◽  
S 100

Preoperative diagnosis of solitary fibrous tumour (SFT) may not provide a complete tumour picture and may be inaccurate. There is no standard treatment for locally advanced or metastasised malignant SFT (MSFT). Here, the case of a 17-year-old male patient with final pathology diagnosis of MSFT is reported. Preoperative biopsy pathology results suggested an Ewing sarcoma that was positive for CD99 antigen, vimentin, friend leukaemia integration 1 transcription factor, apoptosis regulator Bcl-2, and synaptophysin; and negative for CD34 antigen, S-100 protein (S-100), smooth muscle antigen, cytokeratin, and Wilms tumour 1 associated protein. The Ki67 positive rate was 8%, so the patient initially received eight cycles of conversion chemotherapy (vincristine, etoposide, ifosfamide and pirarubicin for one cycle, and vincristine, doxorubicin, and cyclophosphamide/ifosfamide and etoposide for 7 cycles in total). The tumour shrunk significantly and was surgically removed. The final pathology diagnosis was MSFT that was positive for CD99 and signal transducer and activator of transcription 6, and negative for CD34, tumour protein 63, S-100, desmin, and epithelial membrane antigen. Fluorescence in situ hybridization showed no gene translocation in EWS RNA binding protein 1, SS18 subunit of BAF chromatin remodelling complex or FUS RNA binding protein. The patient finally accepted adjuvant radiotherapy of 5600 cGy. Disease-free survival has been > 1 year, with no recurrence or metastasis detected to date. MSFT is rare and treatment for locally advanced or metastatic MSFT remains controversial. The efficacy of the present therapeutic strategy requires further research.

scholarly journals Glial Fibrillary Acidic Protein (GFAP) in Oligodendrogliomas: A Reflection of Transient GFAP Expression by Immature Oligodendroglia

1986 ◽  
Vol 13 (4) ◽  
pp. 307-311 ◽  
Author(s):  
V. Jagadha ◽  
W.C. Halliday ◽  
L.E. Becker
Keyword(s):  
White Matter ◽  
Glial Fibrillary Acidic Protein ◽  
Tumor Cells ◽  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Immunohistochemical Staining ◽  
Neuron Specific Enolase ◽  
Acidic Protein ◽  
Gfap Expression ◽  
S 100

ABSTRACT:Fourteen pure oligodendrogliomas were studied by light- and electronmicroscopy and immunohistochemistry to examine glial fibrillary acidic protein (GFAP) positivity in the tumors. To compare the immunohistochemical staining patterns of neoplastic oligodendroglia and immature oligodendroglia, myelination glia in the white matter of eight normal brains from children under 6 months of age were studied. The tumors possessed light microscopic and ultrastructural features characteristic of oligodendrogliomas. Microtubules were found in the cytoplasm of nine tumors on electronmicroscopy. In one, intermediate filaments and microtubules were observed in occasional tumor cells with polygonal crystalline structures in the cytoplasm. Using the peroxidase-antiperoxidase technique, all specimens were stained for GFAP, vimentin, S-100 and neuron-specific enolase (NSE). In nine tumors, variable numbers of cells with an oligodendroglial morphology reacted positively for GFAP. All tumors were positive for S-100 and negative for vimentin and NSE. The myelination glia in the eight normal brains stained positively for GFAP but not for vimentin. Vimentin is expressed by developing, reactive and neoplastic astrocytes. Thus, GFAP positivity combined with vimentin negativity in both neoplastic and immature oligodendroglia suggests that GFAP positivity in oligodendrogliomas may reflect the transient expression of this intermediate filament by immature oligodendroglia.

Protein S-100 and neuron-specific enolase serum levels remain unaffected by electroconvulsive therapy in patients with depression

2014 ◽  
Vol 121 (11) ◽  
pp. 1411-1415 ◽  
Author(s):  
Laura Kranaster ◽  
Christoph Janke ◽  
Sonani Mindt ◽  
Michael Neumaier ◽  
Alexander Sartorius
Keyword(s):  
Electroconvulsive Therapy ◽  
Neuron Specific Enolase ◽  
Protein S ◽  
Serum Levels ◽  
S 100

Evaluation of Serum Protein S-100 as a New Melanoma Marker

1997 ◽  
pp. 1136-1144
Author(s):  
G. Ghanem ◽  
B. Loir ◽  
J. C. Pector ◽  
P. Ewalenko ◽  
J. Kerger ◽  
...  
Keyword(s):  
Serum Protein ◽  
Protein S ◽  
S 100
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