Enzymic hydrolysis of calf thymus deoxyribonucleic acid adsorbed on diethylaminoethyl cellulose

Biochemistry ◽  
1968 ◽  
Vol 7 (1) ◽  
pp. 406-411 ◽  
Author(s):  
George W. Rushizky ◽  
Isabelle H. Skavenski ◽  
Antoinette E. Greco
Keyword(s):  
Deoxyribonucleic Acid ◽  
Enzymic Hydrolysis ◽  
Calf Thymus ◽  
Diethylaminoethyl Cellulose ◽  
Hydrolysis Of

THE DEGRADATION OF CALF THYMUS DEOXYRIBONUCLEIC ACID BY PANCREATIC DEOXYRIBONUCLEASE

1958 ◽  
Vol 36 (12) ◽  
pp. 1251-1256 ◽  
Author(s):  
R. O. Hurst
Keyword(s):  
Deoxyribonucleic Acid ◽  
Uranyl Acetate ◽  
Calf Thymus ◽  
High Concentrations ◽  
Hydrolysis Of

The hydrolysis of deoxyribonucleic acid by pancreatic deoxyribonuclease was studied using high concentrations of enzyme. An increased production of material soluble in uranyl acetate reagent was obtained. Evidence for heterogeneity in the activity of the enzyme is presented.

THE ACTION OF PANCREATIC DEOXYRIBONUCLEASE ON OLIGONUCLEOTIDES FROM CALF THYMUS DEOXYRIBONUCLEIC ACID

1960 ◽  
Vol 38 (1) ◽  
pp. 347-354 ◽  
Author(s):  
R. O. Hurst ◽  
Dorothy Findlay
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Enzyme Preparation ◽  
Deoxyribonucleic Acid ◽  
Chelating Agent ◽  
Assay Method ◽  
Calf Thymus ◽  
Additional Support ◽  
Hydrolysis Of

Hydrolysis of sodium oligonucleotide by crystalline pancreatic deoxyribonuclease (DNA-ase) has been studied in the presence of different metal ions and the chelating agent ethylenediaminetetraacetate (EDTA). Although EDTA inhibited the action of DNA-ase when magnesium or cobaltous ions were used as activator, the enzyme activity was enhanced in the presence of manganous ions and EDTA. The results are interpreted as indicating the presence of an oligonucleotidase function in the enzyme preparation. A differential assay method for DNA-ase and oligonucleotidase activity has been devised and the evidence obtained gives additional support for this conclusion.

HYDROLYSIS OF CALF THYMUS DEOXYRIBONUCLEATE BY PANCREATIC DEOXYRIBONUCLEASE: I. A STUDY OF THE OLIGONUCLEOTIDES LIBERATED IN THE PRESENCE OF MAGNESIUM OR MANGANESE IONS

1963 ◽  
Vol 41 (1) ◽  
pp. 469-480 ◽  
Author(s):  
R. O. Hurst ◽  
G. C. Becking
Keyword(s):  
Metal Ion ◽  
Deoxyribonucleic Acid ◽  
Exchange Chromatography ◽  
Magnesium Ions ◽  
Manganese Ions ◽  
Terminal Position ◽  
Deoxyribonuclease I ◽  
Calf Thymus ◽  
Hydrolysis Of

The oligonucleotides obtained from deoxyribonucleic acid by the action of pancreatic deoxyribonuclease in the presence of magnesium ions or manganous ions have been analyzed by ion exchange chromatography and by determination of the relative amounts of purine and pyrimidine deoxynucleotides occupying the 5′-terminal position. Evidence of a difference in the specificity of action of the enzyme that is dependent upon the nature of the metal ion activator employed has been adduced.

THE DEGRADATION OF CALF THYMUS DEOXYRIBONUCLEIC ACID BY PANCREATIC DEOXYRIBONUCLEASE

1958 ◽  
Vol 36 (1) ◽  
pp. 1251-1256 ◽  
Author(s):  
R. O. Hurst
Keyword(s):  
Deoxyribonucleic Acid ◽  
Uranyl Acetate ◽  
Calf Thymus ◽  
High Concentrations ◽  
Hydrolysis Of

The hydrolysis of deoxyribonucleic acid by pancreatic deoxyribonuclease was studied using high concentrations of enzyme. An increased production of material soluble in uranyl acetate reagent was obtained. Evidence for heterogeneity in the activity of the enzyme is presented.

THE ACTION OF PANCREATIC DEOXYRIBONUCLEASE ON OLIGONUCLEOTIDES FROM CALF THYMUS DEOXYRIBONUCLEIC ACID

1960 ◽  
Vol 38 (4) ◽  
pp. 347-354 ◽  
Author(s):  
R. O. Hurst ◽  
Dorothy Findlay
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Enzyme Preparation ◽  
Deoxyribonucleic Acid ◽  
Chelating Agent ◽  
Assay Method ◽  
Calf Thymus ◽  
Additional Support ◽  
Hydrolysis Of

Hydrolysis of sodium oligonucleotide by crystalline pancreatic deoxyribonuclease (DNA-ase) has been studied in the presence of different metal ions and the chelating agent ethylenediaminetetraacetate (EDTA). Although EDTA inhibited the action of DNA-ase when magnesium or cobaltous ions were used as activator, the enzyme activity was enhanced in the presence of manganous ions and EDTA. The results are interpreted as indicating the presence of an oligonucleotidase function in the enzyme preparation. A differential assay method for DNA-ase and oligonucleotidase activity has been devised and the evidence obtained gives additional support for this conclusion.

HYDROLYSIS OF CALF THYMUS DEOXYRIBONUCLEATE BY PANCREATIC DEOXYRIBONUCLEASE: I. A STUDY OF THE OLIGONUCLEOTIDES LIBERATED IN THE PRESENCE OF MAGNESIUM OR MANGANESE IONS

1963 ◽  
Vol 41 (2) ◽  
pp. 469-480 ◽  
Author(s):  
R. O. Hurst ◽  
G. C. Becking
Keyword(s):  
Metal Ion ◽  
Deoxyribonucleic Acid ◽  
Exchange Chromatography ◽  
Magnesium Ions ◽  
Manganese Ions ◽  
Terminal Position ◽  
Deoxyribonuclease I ◽  
Calf Thymus ◽  
Hydrolysis Of

The oligonucleotides obtained from deoxyribonucleic acid by the action of pancreatic deoxyribonuclease in the presence of magnesium ions or manganous ions have been analyzed by ion exchange chromatography and by determination of the relative amounts of purine and pyrimidine deoxynucleotides occupying the 5′-terminal position. Evidence of a difference in the specificity of action of the enzyme that is dependent upon the nature of the metal ion activator employed has been adduced.

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