Partial sequence analysis of ribosomal ribonucleic acid from HeLa cells. III. Oligonucleotide pattern after pancreatic ribonuclease digestion and the 3' and 5' termini of 5 S ribonucleic acid from HeLa cells

Biochemistry ◽  
1969 ◽  
Vol 8 (12) ◽  
pp. 4989-5005 ◽  
Author(s):  
L. E. Hatlen ◽  
F. Amaldi ◽  
G. Attardi
Keyword(s):  
Sequence Analysis ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
Partial Sequence ◽  
Pancreatic Ribonuclease ◽  
Ribosomal Ribonucleic Acid

Partial sequence analysis of ribosomal RNA from HeLa cells

1968 ◽  
Vol 33 (3) ◽  
pp. 757-775 ◽  
Author(s):  
Philippe Jeanteur ◽  
Francesco Amaldi ◽  
Giuseppe Attardi
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Rna ◽  
Hela Cells ◽  
Partial Sequence

scholarly journals Biosynthesis of a hypermodified nucleotide in Saccharomyces carlsbergensis 17S and HeLa-cell 18S ribosomal ribonucleic acid

1978 ◽  
Vol 169 (1) ◽  
pp. 71-77 ◽  
Author(s):  
R C Brand ◽  
J Klootwijk ◽  
R J Planta ◽  
B E H Maden
Keyword(s):  
Hela Cell ◽  
Hela Cells ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Saccharomyces Carlsbergensis ◽  
Ribosomal Ribonucleic Acid ◽  
Precursor Molecules

The biosynthesis of a hypermodified nucleotide, similar to or identical with 3-(3-amino-3-carboxypropyl)-1-methylpseudouridine monophosphate, present in Saccharomyces carlsbergensis 17S and HeLa-cell 18S rRNA, was investigated with respect to the sequence of reactions required for synthesis and their timing in ribosome maturation. In both yeast and HeLa cells methylation precedes attachment of the 3-amino-3-carboxypropyl group. In yeast the methylated precursor nucleotide was tentatively characterized as 1-methylpseudouridine. This precursor nucleotide was demonstrated in both 37S and most of the cytoplasmic 18S pre-rRNA (rRNA precursor) molecules. The synthesis of the hypermodified nucleotide is completed just before the final cleavage of 18S pre-rRNA to give 17S rRNA, so that the final addition of the 3-amino-3-carboxypropyl group is a cytoplasmic event. Comparable experiments with HeLa cells indicated that formation of 1-methylpseudouridine occurs at the level of 45S RNA and addition of the 3-amino-3-carboxypropyl group occurs in the cytoplasm on newly synthesized 18S RNA.

Partial sequence analysis of ribosomal RNA from HeLa cells

1968 ◽  
Vol 33 (3) ◽  
pp. 737-755 ◽  
Author(s):  
Francesco Amaldi ◽  
Giuseppe Attardi
Keyword(s):  
Sequence Analysis ◽  
Ribosomal Rna ◽  
Hela Cells ◽  
Partial Sequence

scholarly journals Chemical studies on ribosomal ribonucleic acid and ribosome formation in HeLa cells

1972 ◽  
Vol 129 (3) ◽  
pp. 30P-30P ◽  
Author(s):  
B E H Maden ◽  
M Salim ◽  
R Williamson ◽  
J Shepherd
Keyword(s):  
Hela Cells ◽  
Ribonucleic Acid ◽  
Ribosomal Ribonucleic Acid ◽  
Chemical Studies ◽  
Ribosome Formation
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