Determination of hydration and partial specific volume of proteins with the spring balance

Biochemistry ◽  
1969 ◽  
Vol 8 (7) ◽  
pp. 3077-3082 ◽  
Author(s):  
Rainer Jaenicke ◽  
Max A. Lauffer
Keyword(s):  
Specific Volume ◽  
Partial Specific Volume ◽  
Spring Balance

scholarly journals Determination of partial specific volume and absolute concentration by densimetry

1984 ◽  
Vol 219 (3) ◽  
pp. 1049-1052 ◽  
Author(s):  
P H Stothart
Keyword(s):  
Absorption Coefficient ◽  
Neutron Scattering ◽  
Prior Knowledge ◽  
Small Angle ◽  
Specific Volume ◽  
Small Angle Neutron Scattering ◽  
Partial Specific Volume ◽  
Absolute Concentration ◽  
Non Destructive

The apparent partial specific volume, phi, of a protein (or other macromolecule) and the absolute concentration, c, can be determined from the densimetry of solutions and diffusates in 1H2O, 2H2O and 1H2O/2H2O mixtures if the ratios of concentrations (relative concentrations) are known. The densimetry method allows a non-destructive check on total macromolecule concentrations without prior knowledge of specific u.v.-absorption coefficient. The method may be especially useful in small-angle neutron-scattering studies.

THE DETERMINATION OF PARTIAL SPECIFIC VOLUMES BY DIFFERENTIAL SEDIMENTATION

1956 ◽  
Vol 34 (6) ◽  
pp. 809-814 ◽  
Author(s):  
W. G. Martin ◽  
W. H. Cook ◽  
C. A. Winkler
Keyword(s):  
Polyvinyl Alcohol ◽  
Sodium Alginate ◽  
Heavy Water ◽  
Specific Volume ◽  
Partial Specific Volume ◽  
Plasma Albumin ◽  
Bovine Plasma ◽  
Differential Sedimentation ◽  
Specific Volumes

Sedimentation in aqueous and heavy water solutions has been used to determine the partial specific volume of bovine plasma albumin, sodium alginate, and polyvinyl alcohol, resulting in 0.74 ± 0.01 (S.D.), 0.54 ± 0.06, and 0.79 ± 0.01 ml./gm. at 25 °C, respectively. The sedimentation method yields results comparable with those given by conventional methods for macromolecules.

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