Inhibition by pactamycin of the initiation of protein synthesis. Binding of N-acetylphenylalanyl transfer ribonucleic acid and polyuridylic acid to ribosomes

Biochemistry ◽  
1969 ◽  
Vol 8 (4) ◽  
pp. 1312-1326 ◽  
Author(s):  
Linda B. Cohen ◽  
Albert E. Herner ◽  
Irving H. Goldberg
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Polyuridylic Acid

Protein synthesis by cell-free extracts of Proteus vulgaris

1971 ◽  
Vol 17 (12) ◽  
pp. 1545-1551
Author(s):  
K. L. Backler ◽  
W. E. Inniss
Keyword(s):  
Protein Synthesis ◽  
Ribonucleic Acid ◽  
Supernatant Fraction ◽  
Proteus Vulgaris ◽  
Guanosine Triphosphate ◽  
Cell Extracts ◽  
Polyuridylic Acid ◽  
A Cell ◽  
Directed System ◽  
Low Activity

A cell-free protein-synthesizing system from Proteus vulgaris was developed and characterized. Initially, a very low activity was found to occur; it was shown to be due to a deficiency in the endogenous transfer ribonucleic acid (tRNA) content of the cell extracts. When tRNA was added to the polyuridylic acid (poly U) directed system, incorporation of 14C-phenylalanine into polypeptide readily occurred. This incorporation was dependent on ribosomes and supernatant fraction and the optimum concentrations of the various components of the system were found to be as follows: 120 μg/ml tRNA, 30 mM magnesium, 0.5 mM spermidine, 60 mM potassium, 50 mM adenosine triphosphate (ATP), 5 mM guanosine triphosphate (GTP), 0.03 mM cytosine triphosphate (CTP) and uridine triphosphate (UTP), 5 mM phosphoenolpyruvate (PEP), 50 μg/ml poly U, and 30 mM 2-mercaptoethanol.

scholarly journals Identical 3′-terminal octanucleotide sequence in 18S ribosomal ribonucleic acid from different eukaryotes. A proposed role for this sequence in the recognition of terminator codons

1974 ◽  
Vol 141 (3) ◽  
pp. 609-615 ◽  
Author(s):  
John Shine ◽  
Lynn Dalgarno
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Synthesis ◽  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribonucleic Acid ◽  
18S Rrna ◽  
Base Sequence ◽  
Terminal Sequence ◽  
Ribosomal Ribonucleic Acid ◽  
Rrna Species

The 3′-terminal sequence of 18S ribosomal RNA from Drosophila melanogaster and Saccharomyces cerevisiae was determined by stepwise degradation from the 3′-terminus and labelling with [3H]isoniazid. The sequence G-A-U-C-A-U-U-AOH was found at the 3′-terminus of both 18S rRNA species. Less extensive data for 18S RNA from a number of other eukaryotes are consistent with the same 3′-terminal sequence, and an identical sequence has previously been reported for the 3′-end of rabbit reticulocyte 18S rRNA (Hunt, 1970). These results suggest that the base sequence in this region is strongly conserved and may be identical in all eukaryotes. As the 3′-terminal hexanucleotide is complementary to eukaryotic terminator codons we discuss the possibility that the 3′-end of 18S rRNA may have a direct base-pairing role in the termination of protein synthesis.

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