Isolation and partial characterization of hydroxyproline-rich glycopeptides obtained by enzymic degradation of primary cell walls

Biochemistry ◽  
1969 ◽  
Vol 8 (3) ◽  
pp. 1155-1163 ◽  
Author(s):  
Derek T. A. Lamport ◽  
Leon Clark
Keyword(s):  
Cell Walls ◽  
Primary Cell ◽  
Partial Characterization

Identification and partial characterization of proteins and proteoglycans encrusting the secondary cell walls of flax fibres

Planta ◽  
2000 ◽  
Vol 211 (2) ◽  
pp. 256-264 ◽  
Author(s):  
Raynald Girault ◽  
Isabelle His ◽  
Christine Andeme-Onzighi ◽  
Azeddine Driouich ◽  
Claudine Morvan
Keyword(s):  
Cell Walls ◽  
Partial Characterization ◽  
Flax Fibres ◽  
Secondary Cell Walls

A Single Xyloglucan Xylosyltransferase is Sufficient for Generation of the XXXG Xylosylation Pattern of Xyloglucan

2021 ◽  
Author(s):  
Ruiqin Zhong ◽  
Dennis R Phillips ◽  
Zheng-Hua Ye
Keyword(s):  
Recombinant Proteins ◽  
Cell Walls ◽  
Degree Of Polymerization ◽  
Primary Cell ◽  
Hek293 Cells ◽  
Biochemical Mechanism ◽  
The Third ◽  
Insight Into

Abstarct Xyloglucan is the most abundant hemicellulose in the primary cell walls of dicots. Dicot xyloglucan is the XXXG-type consisting of repeating units of three consecutive xylosylated Glc residues followed by one unsubstituted Glc. Its xylosylation is catalyzed by xyloglucan 6-xylosyltransferases (XXTs) and there exist five XXTs (AtXXT1-5) in Arabidopsis. While AtXXT1and AtXXT2 have been shown to add the first two Xyl residues in the XXXG repeat, which XXTs are responsible for the addition of the third Xyl residue remains elusive although AtXXT5 was a proposed candidate. In this report, we generated recombinant proteins of all five Arabidopsis XXTs and one rice XXT (OsXXT1) in the mammalian HEK293 cells and investigated their ability to sequentially xylosylate Glc residues to generate the XXXG xylosylation pattern. We found that like AtXXT1/2, AtXXT4 and OsXXT1 could efficiently xylosylate the cellohexaose (G6) acceptor to produce mono- and di-xylosylated G6, whereas AtXXT5 was only barely capable of adding one Xyl onto G6. When AtXXT1-catalyzed products were used as acceptors, AtXXT1/2/4 and OsXXT1 but not AtXXT5 were able to xylosylate additional Glc residues to generate tri- and tetra-xylosylated G6. Further characterization of the tri- and tetra-xylosylated G6 revealed that they had the sequence of GXXXGG and GXXXXG with three and four consecutive xylosylated Glc residues, respectively. In addition, we have found that although tri-xylosylation occurred on G6, cello-oligomers with a degree of polymerization of 3 to 5 could only be mono- and di-xylosylated. Together, these results indicate that each of AtXXT1/2/4 and OsXXT1 is capable of sequentially adding Xyl onto three contiguous Glc residues to generate the XXXG xylosylation pattern and these findings provide new insight into the biochemical mechanism underlying xyloglucan biosynthesis.

Extraction and characterization of pectins from primary cell walls of edible açaí (Euterpe oleraceae) berries, fruits of a monocotyledon palm

2017 ◽  
Vol 158 ◽  
pp. 37-43 ◽  
Author(s):  
Thaisa Moro Cantu-Jungles ◽  
Marcello Iacomini ◽  
Thales R. Cipriani ◽  
Lucimara M.C. Cordeiro
Keyword(s):  
Cell Walls ◽  
Primary Cell

Characterization of the primary cell walls of seedlings of Brachypodium distachyon – A potential model plant for temperate grasses

2010 ◽  
Vol 71 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Ulla Christensen ◽  
Ana Alonso-Simon ◽  
Henrik V. Scheller ◽  
William G.T. Willats ◽  
Jesper Harholt
Keyword(s):  
Cell Walls ◽  
Potential Model ◽  
Brachypodium Distachyon ◽  
Primary Cell ◽  
Model Plant

Partial characterization of the cell walls ofMycobacterium leprae

1983 ◽  
Vol 9 (5) ◽  
pp. 269-274 ◽  
Author(s):  
Hugo L. David ◽  
Nalin Rastogi
Keyword(s):  
Cell Walls ◽  
Partial Characterization
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