Characteristics of in vitro ribonucleic acid synthesis by macronuclei of Tetrahymena pyriformis

Biochemistry ◽  
1970 ◽  
Vol 9 (20) ◽  
pp. 3947-3959 ◽  
Author(s):  
Young Chang Lee ◽  
John E. Byfield
Keyword(s):  
Ribonucleic Acid ◽  
Tetrahymena Pyriformis ◽  
Acid Synthesis

scholarly journals Studies on the pathogenesis of liver necrosis by α-amanitin. Effect of α-amanitin on ribonucleic acid synthesis and on ribonucleic acid polymerase in mouse liver nuclei

1967 ◽  
Vol 105 (2) ◽  
pp. 779-782 ◽  
Author(s):  
F. Stirpe ◽  
L. Fiume
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Ribonucleic Acid ◽  
Mouse Liver ◽  
Orotic Acid ◽  
Acid Synthesis ◽  
Liver Necrosis ◽  
Liver Nuclei

1. Injection of α-amanitin to mice causes a decreased incorporation of [6−14C]-orotic acid into liver RNA in vivo. 2. The activity of RNA polymerase activated by Mn2+ and ammonium sulphate is greatly impaired in liver nuclei isolated from mice poisoned with α-amanitin, and is inhibited by the addition of the same toxin in vitro. 3. The activity of the Mg2+-activated RNA polymerase is only slightly affected by α-amanitin either administered to mice or added in vitro.

scholarly journals Rates of aminoacyl-transfer-ribonucleic acid synthesis in vivo and in vitro by bean leaves

1970 ◽  
Vol 117 (5) ◽  
pp. 853-859 ◽  
Author(s):  
T. C. Hall ◽  
K. L. Tao
Keyword(s):  
Amino Acids ◽  
Ribonucleic Acid ◽  
Acid Synthesis ◽  
Leaf Discs ◽  
Endogenous Amino Acids ◽  
Intact Leaves ◽  
Aminoacyl Transfer ◽  
Bean Leaves

1. A procedure for measuring rates of aminoacyl-tRNA synthesis in vitro and in intact leaves is presented. 2. Leaf discs showed rates close to those of intact leaves. 3. Cell-free preparations showed similar rates when assayed by pyrophosphate exchange, but actual aminoacyl-tRNA formation rates appeared to be much lower. Evidence is presented that dilution of supplied labelled amino acids was a major factor causing the low apparent rates. 4. Attempts to strip endogenous amino acids from plant tRNA resulted in low acceptor capability of the tRNA.

scholarly journals Selective resistance to desiccation of nuclear ribonucleic acid synthesis in isolated nuclei of Artemia salina embryos during pre-emergence development

1976 ◽  
Vol 158 (3) ◽  
pp. 639-642 ◽  
Author(s):  
D D Chaffoy ◽  
M Kondo
Keyword(s):  
Incubation Period ◽  
Ribonucleic Acid ◽  
Transcriptional Activity ◽  
Time Lag ◽  
Acid Synthesis ◽  
Artemia Salina ◽  
The Other ◽  
Considerable Time ◽  
Isolated Nuclei

The developing gastrula embryos of Artemia salina are resistant to a complete redesiccation during a period of pre-emergence development. Isolated nuclei from these dehydrated embryos could retain a transcriptional activity in vitro comparable with that of non-desiccated controls. On the other hand, redesiccation of both prenauplii and nauplii completely destroys their viability as well as the nuclear transcriptional activity. However, those gastrula embryos that did not develop in a first incubation period could remain viable and develop with a considerable time-lag after a subsequent second incubation.

Effect of 8-methoxypsoralen and 4,5′- dimethylangelicin on ribonucleic acid synthesis in vitro

1988 ◽  
Vol 37 (9) ◽  
pp. 1827-1828
Author(s):  
Małgorzata Czyż ◽  
Katarzyna Piestrzeniewicz ◽  
Dorota Wilmańska ◽  
Kazimierz Studzian ◽  
Leszek Szmigiero ◽  
...  
Keyword(s):  
Ribonucleic Acid ◽  
Acid Synthesis
Sign in / Sign up

Export Citation Format

Share Document