Circular dichroic and optical rotatory dispersion spectra of the threonine-inhibited aspartokinase-homoserine dehydrogenase of Escherichia coli K 12. Effects of ligand binding and protein denaturation

Biochemistry ◽  
1970 ◽  
Vol 9 (14) ◽  
pp. 2776-2783 ◽  
Author(s):  
Henry d'A. Heck ◽  
Paolo Truffa-Bachi
Keyword(s):  
Escherichia Coli ◽  
Ligand Binding ◽  
Protein Denaturation ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Homoserine Dehydrogenase ◽  
K 12 ◽  
Circular Dichroic

The effect of ligand binding and acid splitting on the optical rotatory dispersion of ferric horseradish peroxidase

1968 ◽  
Vol 46 (10) ◽  
pp. 1231-1235 ◽  
Author(s):  
William D. Ellis ◽  
H. Brian Dunford
Keyword(s):  
Horseradish Peroxidase ◽  
Ligand Binding ◽  
Protein Conformation ◽  
Spectral Region ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Helical Content ◽  
Protein Moiety ◽  
Soret Region

The optical rotatory dispersion of horseradish peroxidase and its cyanide, fluoride, and hydroxide complexes was studied in the spectral region 215–450 mμ, and that of the azide complex at 350–450 mμ. The effect that splitting the heme from the protein of peroxidase has on the optical rotatory dispersion in the 215–450 mμ region was also studied. Results of measurements of the reduced mean residue rotation at 233 mμ, lead to the conclusion that there are no significant changes in gross protein conformation upon the binding of ligands to peroxidase, but that the splitting of the heme causes a reduction of the helical content of the protein. Pure peroxidase was estimated to have 43% α-helical content, which was reduced to 33% when the heme was split from the protein. Results of studies in the Soret region indicate that the binding of various ligands does not cause an alteration of the geometry of the heme with respect to the protein moiety.

Alkaloids of Lycopodiumlucidulum Michx. Structure and properties of alkaloid L.23

1969 ◽  
Vol 47 (3) ◽  
pp. 449-455 ◽  
Author(s):  
W. A. Ayer ◽  
B. Altenkirk ◽  
R. H. Burnell ◽  
M. Moinas
Keyword(s):  
Absolute Configuration ◽  
Cotton Effect ◽  
Ultraviolet Spectrum ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Structure And Properties ◽  
The Absolute ◽  
Related Compounds ◽  
Circular Dichroic

The minor alkaloids present among the strong bases of Lycopodiumlucidulum have been reinvestigated. Manske and Marion's alkaloid L.23 has been shown to be epimeric with lycodoline at C-12 and the nitrogen. A correlation between the two alkaloids has been achieved. The presence of a σ-coupled p interaction in the ultraviolet spectrum of lycopodine, lycodoline, and related compounds is described. A Cotton effect due to this chromophore has been detected by means of circular dichroism. The optical rotatory dispersion and circular dichroic properties of lycopodine, 12-epilycopodine, lycodoline, and alkaloid L.23 are discussed in terms of the absolute configuration assigned to these alkaloids.

On the use of the n-propylammonium and n-butylammonium salts of d-10-camphorsulfonic acid for the calibration of spectropolarimeters: the concentration and solvent dependency of the optical rotatory dispersion parameters

1976 ◽  
Vol 54 (20) ◽  
pp. 3200-3202
Author(s):  
Yasushi Nakagawa ◽  
Michael F. Gillen ◽  
Ross E. Williams
Keyword(s):  
Ammonium Salts ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Dispersion Parameters ◽  
Camphorsulfonic Acid ◽  
Concentration Dependency ◽  
Circular Dichroic

The utility of the title ammonium salts for the calibration of ord spectropolarimeters has been investigated. The solvent and concentration dependency of the measured specific molar rotations at 306 and 270 nm suggest that they may be used for calibration purposes only under well-defined conditions. The results of the Kramers–Kronig transformation of their circular dichroic spectra are presented and compared with the measured ord spectra.

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