Composition and structure of plasma lipoproteins. Isolation, composition, and immunochemical characterization of low density lipoprotein subfractions of human plasma

Biochemistry ◽  
1970 ◽  
Vol 9 (11) ◽  
pp. 2244-2252 ◽  
Author(s):  
Diana M. Lee ◽  
Petar Alaupovic
Keyword(s):  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Lipoprotein Subfractions ◽  
Immunochemical Characterization ◽  
Composition And Structure

Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

1976 ◽  
Vol 35 (01) ◽  
pp. 178-185 ◽  
Author(s):  
Helena Sandberg ◽  
Lars-Olov Andersson
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Platelet Rich Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Free Plasma ◽  
Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

scholarly journals Fluorescence studies of protein–sterol relationships in human plasma lipoproteins

1974 ◽  
Vol 137 (2) ◽  
pp. 413-415 ◽  
Author(s):  
Rory J. M. Smith ◽  
Colin Green
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Tryptophan Fluorescence ◽  
High Density ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Fluorescence Studies ◽  
Esterified Sterols

Cholesta-5,7,9(11)-trien-3β-ol and its oleate ester were incorporated into human low-density lipoprotein and reconstituted high-density lipoprotein. The unesterified sterol was more efficient than its ester in quenching tryptophan fluorescence, especially in low-density lipoprotein. The results, which indicate that in such lipoproteins unesterified sterols are more closely associated with peptide than are esterified sterols, are used to assess possible structures for the lipoproteins.

Immunochemical Characterization of Purified Human Oxidized Low-Density Lipoprotein Antibodies

2000 ◽  
Vol 95 (2) ◽  
pp. 135-144 ◽  
Author(s):  
G. Virella ◽  
S. Koskinen ◽  
G. Krings ◽  
J.M. Onorato ◽  
S.R. Thorpe ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Oxidized Low Density Lipoprotein ◽  
Immunochemical Characterization

Characterization of modified low density lipoprotein subfractions by capillary isotachophoresis

2001 ◽  
Vol 22 (6) ◽  
pp. 1143-1149 ◽  
Author(s):  
Ulrike Zorn ◽  
Cornelia Haug ◽  
Engin Celik ◽  
Roman Wennauer ◽  
Alexandra Schmid-Kotsas ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Capillary Isotachophoresis ◽  
Lipoprotein Subfractions

Proteome of human plasma very low-density lipoprotein and low-density lipoprotein exhibits a link with coagulation and lipid metabolism

2014 ◽  
Vol 111 (03) ◽  
pp. 518-530 ◽  
Author(s):  
Monireh Dashty ◽  
Mohammad Motazacker ◽  
Johannes Levels ◽  
Marcel de Vries ◽  
Morteza Mahmoudi ◽  
...  
Keyword(s):  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Protein S ◽  
The Body ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Coagulation Proteins ◽  
Associated Proteins

SummaryApart from transporting lipids through the body, the human plasma lipoproteins very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) are also thought to serve as a modality for intra-organismal protein transfer, shipping proteins with important roles in inflammation and thrombosis from the site of synthesis to effector locations. To better understand the role of VLDL and LDL in the transport of proteins, we applied a combination of LTQ ORBITRAP-XL (nLC-MS/MS) with both in-SDS-PAGE gel and in-solution tryptic digestion of pure and defined VLDL and LDL fractions. We identified the presence of 95 VLDL-and 51 LDL-associated proteins including all known apolipoproteins and lipid transport proteins, and intriguingly a set of coagulation proteins, complement system and anti-microbial proteins. Prothrombin, protein S, fibrinogen γ, PLTP, CETP, CD14 and LBP were present on VLDL but not on LDL. Prenylcysteine oxidase 1, dermcidin, cathelicidin antimicrobial peptide, TFPI-1 and fibrinogen α chain were associated with both VLDL and LDL. Apo A-V is only present on VLDL and not on LDL. Collectively, this study provides a wealth of knowledge on the protein constituents of the human plasma lipoprotein system and strongly supports the notion that protein shuttling through this system is involved in the regulation of biological processes. Human diseases related to proteins carried by VLDL and LDL can be divided in three major categories: 1 – dyslipidaemia, 2 – atherosclerosis and vascular disease, and 3 – coagulation disorders.

Differential precipitation of isolated human plasma lipoproteins with heparin and manganese chloride.

1988 ◽  
Vol 34 (2) ◽  
pp. 240-243 ◽  
Author(s):  
J M Ruiz-Albusac ◽  
E Velázquez ◽  
A Montes
Keyword(s):  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Serum Lipoproteins ◽  
Supernatant Liquid ◽  
Differential Precipitation ◽  
Preparative Ultracentrifugation

Abstract We studied the precipitation of isolated lipoproteins with heparin and MnCl2. Lipoproteins were isolated from human plasma by preparative ultracentrifugation and their free cholesterol was labeled. Each lipoprotein fraction was then precipitated at various pHs, with or without bovine serum albumin (60 g/L) present. Under no set of conditions was one class of lipoproteins completely separated from the other two. Specifically, under standard conditions for precipitation of serum lipoproteins (pH 7.4 and protein 60 g/L), 12% of the very-low-density lipoprotein (VLDL) and 8% of the low-density lipoprotein (LDL) remained in the supernatant liquid, and 30% of the high-density lipoprotein (HDL) was precipitated. These results indicate that, under these conditions, so-called HDL cholesterol may be a mixture of VLDL, LDL, and HDL, although the sum of the amount of these three fractions remaining in the supernate is fortuitously very close to the value for HDL cholesterol isolated by ultracentrifugation.

Short-Term Effect of Various Doses of Cyclosporin A on Plasma Lipoproteins and its Distribution in Blood: An Experimental Study

1993 ◽  
Vol 12 (2) ◽  
pp. 141-146 ◽  
Author(s):  
R.J. Andrade ◽  
M.I. Lucena ◽  
J.A. Gonzalez-Correa ◽  
C. Garcia-Arias ◽  
P. Gonzalez-Santos
Keyword(s):  
Cyclosporin A ◽  
Whole Blood ◽  
Plasma Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Short Term ◽  
Short Term Treatment ◽  
Lipoprotein Subfractions

Hyperlipidaemia commonly develops in both transplant recipients and experimental animals receiving cyclosporin A (CsA). However, the threshold of CsA induced-changes on lipoproteins and the role of parenteral vehicle (cremophor) has not been defined. Male Wistar rats were classified into five groups of six animals each and received CsA in cremophor vehicle at doses of 5, 10 or 20 mg kg-1 d-1, s.c., vehicle alone or saline for 7 d. Blood was obtained 24 h after the last dose and plasma was analysed. Plasma very low density lipoprotein (VLDL), low density lipoprotein (LDL), and high density lipoprotein subfractions (HDL-2, HDL-3) were isolated by sequential ultracentrifugation and their content of cholesterol, triglyceride and phospholipid was determined. Whole blood and trough plasma CsA levels were measured by monoclonal radioimmunoassay. Plasma lipids did not differ significantly among the five groups. At a dose of 20 mg kg-1 d-1 of CsA VLDL cholesterol rose significantly (P<0.05). Administration of either CsA or cremophor vehicle increased HDL-2 phospholipids (P<0.05) and decreased HDL-3 cholesterol. There was not a linear relationship between whole blood and plasma CsA levels and increasing CsA doses. Short-term treatment with low doses of CsA have little influence on lipid profile in the rat. Changes on lipoprotein composition can be attributed mainly to cremophor vehicle, conceivably due to its ethanol content.

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