Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus

Biochemistry ◽  
1970 ◽  
Vol 9 (4) ◽  
pp. 869-877 ◽  
Author(s):  
Marilynn E. Etzler ◽  
Elvin A. Kabat
Keyword(s):  
Blood Group ◽  
Purification And Characterization ◽  
Dolichos Biflorus ◽  
Blood Group A ◽  
Group A

scholarly journals Isolation and characterization of novel glycolipids with blood group A-related structures: galactosyl-A and sialosylgalactosyl-A.

1987 ◽  
Vol 262 (29) ◽  
pp. 14228-14234
Author(s):  
H Clausen ◽  
S B Levery ◽  
E D Nudelman ◽  
M Stroud ◽  
M E Salyan ◽  
...  
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Isolation And Characterization ◽  
Group A

scholarly journals Immunochemical studies on blood groups LXII. Fractionation of hog and human A, H, and AH blood group active substance on insoluble immunoadsorbents of Dolichos and Lotus lectins.

1976 ◽  
Vol 143 (2) ◽  
pp. 422-436 ◽  
Author(s):  
M E Pereira ◽  
E A Kabat
Keyword(s):  
Blood Group ◽  
Optical Rotation ◽  
Gastric Mucin ◽  
Specific Optical Rotation ◽  
Dolichos Biflorus ◽  
Blood Group A ◽  
Analytical Properties ◽  
Group Substance ◽  
Group A

The purified lectins from Lotus tetragonolobus and Dolichos biflorus were coupled to Sepharose 2B to make insoluble adsorbents for purification and fractionation of blood group A and H active glycoproteins. With both adsorbents, hog gastric mucin A + H blood substance (HGM), purified by phenol-ethanol precipitation, yielded fractions showing only A, only H, or AH activities. The AH fraction was obtained when the adsorbent column was overloaded with HGM and its A and H specificities seem to be carried on the same molecules since they were not separable by chromatography on either column. However A and H specificities of blood group substance from the stomach of a presumably heterozygous individual hog were both on the same molecules as they too could not be fractionated on either column. Analytical properties of the isolated fractions were generally similar to those of the unfractionated material, the purfied A substances had a higher galactosamine/fucose ratio than did the H substances. Although the original A + H showed very little specific optical rotation, the separated A and H substances rotated positively and negatively, respectively. The lectin-Sepharose adsorbents have also proven useful in isolating A or H substances directly from the crude commercial hog gastric mucin. Blood group A2 substance from a human ovarian cyst yielded two fractions on the Lotus-Sepharose column; the effluent did not interact with the Lotus lectin but precipitated the Ulex and Dolichos lectins and anti-A, and appears to contain type 1 H determinants. The other fraction reacted with Lotus and Ulex lectin as well as with Dolichos and anti-A.

scholarly journals Gambaran Populasi Golongan Darah Subgroup A (A1, A2) di PMI Kulon Progo

Biosfera ◽  
2017 ◽  
Vol 34 (1) ◽  
pp. 47
Author(s):  
Hieronymus Rayi Prasetya ◽  
Bambang Heru Budianto ◽  
Hernayanti Hernayanti
Keyword(s):  
Genetic Variation ◽  
Blood Group ◽  
Sampling Technique ◽  
Red Cross ◽  
Cross Sectional ◽  
Dolichos Biflorus ◽  
Blood Group A ◽  
Group A ◽  
Transfusion Reactions

Subgroup A1 and A2 are the most important in the blood group A. Subgroup A1 has the A antigen more than A2 subgroup, the A2 subgroup can cause misidentification of blood group due to poor A antigen and genetic variation possessed. Misidentification of the blood group will increase the risk of transfusion reactions. This research aims to describe the A1 and A2 subgroup population in Kulon Progo district. This study was conducted with a cross sectional sampling technique. The sample in this study were taken from donors of blood group A in Kulon Progo Red Cross. Identification of A1 and A2 subgroup is done by using lectin (Dolichos biflorus extract). The result of the examination of 53 samples showed that 96,2% was A1 subgroup and 3,8% was A2 subgroup.Key words : Subgroup A1, Subgroup A2, Population, Kulon Progo

Isolation and Characterization of a Blood Group A Substance-degrading α-N-Acetylgalactosaminidase from anAcremoniumsp.

1989 ◽  
Vol 53 (1) ◽  
pp. 111-120 ◽  
Author(s):  
Setsu Kadowaki ◽  
Takeshi Ueda ◽  
Kenji Yamamoto ◽  
Hidehiko Kumagai ◽  
Tatsurokuro Tochikura
Keyword(s):  
Blood Group ◽  
Blood Group A ◽  
Isolation And Characterization ◽  
Group A
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