Amino acid sequence of bovine carboxypeptidase A. Isolation and characterization of the thermolytic peptides of the cyanogen bromide fragment FI

Biochemistry ◽  
1971 ◽  
Vol 10 (6) ◽  
pp. 951-961
Author(s):  
Hans Neurath ◽  
Ralph A. Bradshaw ◽  
Kenneth A. Walsh
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Carboxypeptidase A ◽  
Isolation And Characterization ◽  
Cyanogen Bromide Fragment

Amino acid sequence of thermolysin. Isolation and characterization of the fragments obtained by cleavage with cyanogen bromide

Biochemistry ◽  
1972 ◽  
Vol 11 (13) ◽  
pp. 2427-2435 ◽  
Author(s):  
Koiti Titani ◽  
Mark A. Hermodson ◽  
Lowell H. Ericsson ◽  
Kenneth A. Walsh ◽  
Hans Neurath
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Isolation And Characterization

Amino acid sequence of penicillopepsin. III. Isolation and characterization of amino terminal, tryptic, and tryptophanyl peptides

1976 ◽  
Vol 54 (10) ◽  
pp. 895-901
Author(s):  
C. Ian Harris ◽  
Leticia Rao ◽  
Paul Shutsa ◽  
Alexander Kurosky ◽  
Theo Hofmann
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Amino Acid Sequences ◽  
Affinity Column ◽  
Amino Terminal ◽  
Tryptic Digest ◽  
The Third ◽  
Isolation And Characterization

The amino acid sequences of peptides isolated from a tryptic digest of penicillopepsin (EC 3.4.23.7), a subtilisin (EC 3.4.21.14) digest of maleylated penicillopepsin, and a chymotryptic digest of penicillopepsin modified with dinitrophenylsulfenyl (DNPS) chloride have been determined. The first two digests identified four of the five lysyl residues of the enzyme as well as the N-terminal peptide. The third digest provided overlaps at three of the tryptophanyl residues. The DNPS-tryptophan peptides were isolated on an affinity column prepared by coupling dinitrophenyl antibody raised in sheep to cyanogen bromide-activated Sepharose.

Amino acid sequence of bovine carboxypeptidase A. II. Tryptic and chymotryptic peptides of the cyanogen bromide fragment F111

Biochemistry ◽  
1969 ◽  
Vol 8 (9) ◽  
pp. 3859-3871 ◽  
Author(s):  
Ralph A. Bradshaw ◽  
Donald R. Babin ◽  
Masao Nomoto ◽  
N. G. Srinivasin ◽  
Lowell H. Ericsson ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Carboxypeptidase A ◽  
Cyanogen Bromide Fragment

Amino acid sequence of cyanogen bromide fragment CB3 of hog pepsin

1981 ◽  
Vol 46 (3) ◽  
pp. 655-666
Author(s):  
Ladislav Morávek ◽  
Vladimír Kostka
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Methionine Residues ◽  
Cyanogen Bromide Fragment

On the basis of the knowlidge of thermolytic, chymotryptic and substilisin peptides the amino acid sequence was determined of cyanogen bromide fragment CB3 representing the region between methionine residues I and II of pepsin: Thr-Gly-Ile-Leu-Gly-Tyr-Asp-Thr-Val-Gln-Val-Gly-Gly-Ile-Ser-Asp-Thr-Asn-Gln-Ile-Phe-Gly-Leu-Ser-Glu-Thr-Glu-Pro-Gly-Ser-Phe-Leu-Tyr-Tyr-Ala-Pro-Phe-Asp-Gly-Ile-Leu-Gly-Leu-Ala-Tyr-Pro-Ser-Ile-Ser-Ala-Ser-Gly-Ala-Thr-Pro-Val-Phe-Asp-Asn-Leu-Trp-Asp-Gln-Gly-Leu-Val-Ser-Gln-Asp-Leu-Phe-Ser-Val-Tyr-Leu-Ser-Ser-Asn-Asp-Asp-Ser-Gly-Ser-Val-Val-Leu-Leu-Gly-Gly-Ile-Asp-Ser-Ser-Tyr-Tyr-Thr-Gly-Ser-Leu-Asn-Trp-Val-Pro-Val-Ser-Val-Glu-Gly-Tyr-Trp-Gln-Ile-Thr-Leu-Asp-Ser-Ile-Thr-Met.

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