Synthesis and decay rates of major classes of deoxyribonucleic acid-like ribonucleic acid in sea urchin embryos

Biochemistry ◽  
1971 ◽  
Vol 10 (5) ◽  
pp. 877-881 ◽  
Author(s):  
Bruce P. Brandhorst ◽  
Tom Humphreys
Keyword(s):  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Decay Rates ◽  
Sea Urchin Embryos

EFFECT OF OESTRADIOL-17β ON CLEAVAGE, NUCLEIC ACID METABOLISM AND PROTEIN SYNTHESIS IN SEA URCHIN, STRONGYLOCENTROTUS PURPURATUS, EMBRYOS

1962 ◽  
Vol 25 (2) ◽  
pp. 183-NP ◽  
Author(s):  
W. B. JOLLEY ◽  
W. E. MARTIN ◽  
J. W. BAMBERGER ◽  
L. W. STEARNS
Keyword(s):  
Protein Synthesis ◽  
Nucleic Acid ◽  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Acid Metabolism ◽  
Strongylocentrotus Purpuratus ◽  
Nucleic Acid Metabolism ◽  
Dna And Rna ◽  
Moderate Effect

SUMMARY Oestradiol-17β at a concentration of 3 × 10−3 m inhibits cleavage in sea urchin (Strongylocentrotus purpuratus) embryos. This inhibition is accompanied by a reduction in deoxyribonucleic acid (DNA) synthesis and little change in ribonucleic acid (RNA). The effects of oestradiol-17β upon the incorporation of glycine-1-14C and glycine-2-14C into the purines of DNA and RNA and the incorporation of glycine-2-14C into serine were studied. The incorporation of glycine-1-14C and glycine-2-14C into RNA was reduced, but the incorporation of glycine-2-14C into DNA was increased considerably over that of the controls. The incorporation of glycine-2-14C into serine was also accelerated by oestradiol. A possible explanation of the action of oestradiol-17β is offered. The moderate effect upon RNA is not surprising because there is little or no synthesis of this compound from the time of fertilization to blastulation under normal conditions.

Double-stranded ribonucleic acid in sea urchin embryos

Biochemistry ◽  
1972 ◽  
Vol 11 (11) ◽  
pp. 2020-2026 ◽  
Author(s):  
Lee H. Kronenberg ◽  
Tom Humphreys
Keyword(s):  
Sea Urchin ◽  
Ribonucleic Acid ◽  
Sea Urchin Embryos
Sign in / Sign up

Export Citation Format

Share Document