Transcription and processing of ribonucleic acid in Rhynchosciara salivary glands. II. Hybridization of nuclear and cytoplasmic ribonucleic acid with nuclear deoxyribonucleic acid. Indication of deoxyribonucleic acid amplification

Biochemistry ◽  
1972 ◽  
Vol 11 (20) ◽  
pp. 3672-3680 ◽  
Author(s):  
Hugo A. Armelin ◽  
Nelson Marques
1970 ◽  
Vol 18 (7) ◽  
pp. 510-514 ◽  
Author(s):  
WILLIAM H. J. DOUGLAS

A method is presented for the extraction of deoxyribonucleic acid from thin sections of tissue fixed in glutaraldehyde and osmium tetroxide and embedded in water-insoluble epoxy resin. Incubation of thin sections in 20% perchloric acid at 60°C for 8 hr produced electron-transparent bands in the polytene chromosomes from Drosophila salivary glands. Chromosome bands were also rendered electron-transparent when hydroxypropylmethacrylate-embedded thin sections were digested with deoxyribonuclease or extracted with perchloric acid. Nucleolar ribonucleic acid was unaffected by the perchloric acid extraction.


Biochemistry ◽  
1979 ◽  
Vol 18 (21) ◽  
pp. 4600-4605 ◽  
Author(s):  
Robert P. Martin ◽  
Jean Marie Schneller ◽  
Andre J. C. Stahl ◽  
Guy Dirheimer

1975 ◽  
Vol 250 (20) ◽  
pp. 8179-8183
Author(s):  
WE Lynch ◽  
S Surrey ◽  
I Lieberman

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