Fluorescence polarization studies of the self-association of beef liver glutamate dehydrogenase

Biochemistry ◽  
1972 ◽  
Vol 11 (16) ◽  
pp. 3022-3027 ◽  
Author(s):  
Dean A. Malencik ◽  
Sonia R. Anderson
Keyword(s):  
Glutamate Dehydrogenase ◽  
Fluorescence Polarization ◽  
The Self ◽  
Beef Liver ◽  
Polarization Studies ◽  
Self Association

The Effect of Guanidinium Chloride on the Self-Association of Bovine Liver Glutamate Dehydrogenase: A Gel Filtration Study

1987 ◽  
Vol 42 (3) ◽  
pp. 217-220
Author(s):  
Alberto Mazzini ◽  
Roberto Favilla
Keyword(s):  
Sodium Chloride ◽  
Glutamate Dehydrogenase ◽  
Gel Filtration ◽  
Bovine Liver ◽  
The Self ◽  
Gel Chromatography ◽  
Elution Volume ◽  
Guanidinium Chloride ◽  
Trimer Formation ◽  
Self Association

The associative behaviour of bovine liver glutamate dehydrogenase has been studied by gel chromatography at neutral pH in 1 ᴍ guanidinium chloride and 1 ᴍ sodium chloride. In guanidinium chloride both the elution volume and the elution profile of the enzyme are independ­ent of protein concentration, whereas in sodium chloride they are strongly dependent on it. In NaCl the enzyme behaves as expected according to the well established random association model, whereas in guanidinium chloride it appears to have completely lost the self-associative property. Furthermore, since the elution volume of the enzyme in guanidinium chloride corre­sponds to that of an hexamer, trimer formation reported to occur in these conditions is not confirmed by this technique.

Monte Carlo Study of the Self-association of Methanol in Benzene

1991 ◽  
Vol 6 (4-6) ◽  
pp. 299-310 ◽  
Author(s):  
Y. Adachi ◽  
K. Nakanishi
Keyword(s):  
Monte Carlo ◽  
Monte Carlo Study ◽  
The Self ◽  
Self Association
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