Chemical modification of transfer ribonucleic acid species. Thallium(III)-mediated iodination of yeast formylatable methionine transfer ribonucleic acid

Biochemistry ◽  
1973 ◽  
Vol 12 (24) ◽  
pp. 4980-4983 ◽  
Author(s):  
Francis J. Schmidt ◽  
Daniel R. Omilianowski ◽  
Robert M. Bock
Keyword(s):  
Chemical Modification ◽  
Ribonucleic Acid

scholarly journals Chemical modification as a probe of conformational changes in transfer ribonucleic acid on aminoacylation

1978 ◽  
Vol 171 (3) ◽  
pp. 601-606
Author(s):  
M Lowdon ◽  
J P Goddard
Keyword(s):  
Escherichia Coli ◽  
Chemical Modification ◽  
Conformational Change ◽  
Conformational Changes ◽  
Ribonucleic Acid ◽  
Previous Evidence ◽  
Base Modification ◽  
Trna Species

Treatment of Escherichia coli CA265 phenylalanyl-tRNA with 3M-NaHSO3, pH6.0, at 25 degrees C resulted in modification of four bases and in the deacylation of the charged tRNAphe. The similarity of the rates of base modification and of the deacylation of the phenylalanyl-tRNA permitted the isolation of partially modified phenylalanyl-tRNAphe and partially modified deacylated tRNAphe. The sites and extents of base modification in these fractions were determined and found to be the same as those in uncharged tRNAphe modified under identical conditions. These findings are discussed in relation to previous evidence for and against a conformational change in tRNA on its aminoacylation. The methods described should prove adaptable to study of other aminoacyl-tRNA species.

Chemical modification of the fluorescent base in phenylalanine transfer ribonucleic acid

Biochemistry ◽  
1971 ◽  
Vol 10 (15) ◽  
pp. 2969-2976 ◽  
Author(s):  
Elizabeth B. Keller ◽  
Doju Yoshikami
Keyword(s):  
Chemical Modification ◽  
Ribonucleic Acid
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