New deoxyribonucleic acid dependent deoxyribonucleic acid polymerase from HeLa cell mitochondria

Biochemistry ◽  
1973 ◽  
Vol 12 (19) ◽  
pp. 3602-3608 ◽  
Author(s):  
Michael Fry ◽  
Arthur Weissbach
1974 ◽  
Vol 249 (18) ◽  
pp. 5809-5815
Author(s):  
Silvio Spadari ◽  
Arthur Weissbach

1970 ◽  
Vol 45 (3) ◽  
pp. 509-513 ◽  
Author(s):  
Thoru Pederson ◽  
Elliott Robbins

The cell-free synthesis of histone-like polypeptides has been achieved using a selected class of small polyribosomes as the only particulate fraction. This synthesis is prevented if the deoxyribonucleic acid (DNA) inhibitor, cytosine arabinoside, is added to the cells prior to disruption, and it is not detected when the cytoplasm used is derived from postmitotic (G1) cells. When the 100,000 g supernate from pure metaphase populations was compared with that from S phase cells, the cell-free synthesis of histone-like polypeptides in the presence of S phase polyribosomes remained unchanged. These data suggest that, except for the histone messenger RNA-ribosome complex, the cytoplasmic factors requisite for histone synthesis are present throughout the cycle, and that the shut-off of this synthesis is not under translational control.


1961 ◽  
Vol 9 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Ellen Borenfreund ◽  
Aaron Bendich

Tritium-labeled deoxyribonucleic acid (DNA) from pneumococci and from human leukocytes was added to growing cultures of HeLa cells at 37°C. Autoradiography revealed an extensive localization of tritium in the nuclear regions. The label could not be removed by treatment with ribonuclease or dilute perchloric acid, but quantitative removal from the cells could be effected with deoxyribonuclease. Chemical and radioactivity determinations on nucleic acids isolated from the exposed HeLa cells revealed the presence of tritium in all 4 DNA bases. About 12 µg. of tritiated DNA was recovered from 6 x 106 HeLa cells which had been exposed for 24 hours to 240 µg. of the human DNA. From this, it is concluded that the amount of DNA, or its degradation products, taken up by the cells was equivalent to at least 10 per cent of the normal HeLa cell complement.


2016 ◽  
Vol 2 (2) ◽  
pp. 1-30 ◽  
Author(s):  
Sandra Harvey

This essay reads the narratives of HeLa cell contamination as accusations of racial and gender passing. It argues that the passing narrative is much more complex, rarely confined to an individual’s autonomous will, and far more entrenched in state building and concepts of social progress than previously considered. I urge us to move away from the desire of the passing subject, and back to our own to ask after the sort of anxiety, excitement, and panic that animate our attempts to see, classify, and regulate bodies. Thus, what becomes significant is an examination of an “ethics of knowing” within science. The paper draws on a collection of correspondence, lab notes, published articles, and newspaper clippings related to Henrietta Lacks and HeLa from the George O. Gey Collection at the Medical Archives of the Johns Hopkins Medical Institutions (1918-1974) and articles on HeLa published in scientific journals, science journalism, and cultural studies articles (1950-present). In doing so, it traces the narratives of science (and its complex of industries—journalism and cultural studies) and HeLa’s passing. Tracing the reactions to HeLa contamination, the paper asks after the ways national, racial, and sexual desire, fantasy, anxiety, and paranoia have animated the cells through time. Particularly it examines the agency of HeLa, a cell line that is passed through race and genders and ideas of mortality, as it makes clear its own vital, creative, and destructive forces.


2011 ◽  
Vol 3 (1) ◽  
Author(s):  
Lies Indah Sutiknowati

There is an information how to identify hydrocarbon degrading bacteria for bioremediation of marine oil spill. We have Bioremediation treatment for degradation of oil spill on Pari island and need two kind of experiment there are tanks experiment (sampling 0 to 90 days) and semi enclosed system (sampling 0 to 150 days). Biostimulation with nutrients (N and P) was done to analyze biodegradation of hydrocarbon compounds. Experiment design using fertilizer Super IB and Linstar will stimulate bacteria can degrade oil, n-alkane, and alkane as poly aromatic hydrocarbon. The bacteria communities were monitored and analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Clone Library; oil chemistry was analyzed by Gas Chromatography Mass Spectrometry (GCMS). DNA (deoxyribonucleic acid) was extracted from colonies of bacteria and sequence determination of the 16S rDNA was amplified by primers U515f and U1492r. Strains had been sequence and had similarity about 90-99% to their closest taxa by homology Blast search and few of them suspected as new species. The results showed that fertilizers gave a significant effect on alkane, PAH and oil degradation in tanks experiment but not in the field test. Dominant of the specific bacteria on this experiment were Alcanivorax, Marinobacter and Prosthecochloris. Keywords: Bioremediation, Biostimulation, DGGE, PAH, Pari Island


2019 ◽  
Vol 7 (4) ◽  
pp. 91-96
Author(s):  
Isra'a Al-sobhi ◽  
◽  
Rawan Al-Ghabban ◽  
Soad Shaker Ali ◽  
Jehan Al-Amri ◽  
...  

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