Spectral changes on binding of oligosaccharides to murine immunoglobulin A myeloma proteins

Biochemistry ◽  
1973 ◽  
Vol 12 (16) ◽  
pp. 3039-3044 ◽  
Author(s):  
M. E. Jolley ◽  
S. Rudikoff ◽  
Michael Potter ◽  
C. P. J. Glaudemans
Keyword(s):  
Immunoglobulin A ◽  
Spectral Changes ◽  
Myeloma Proteins

Correction - Spectral Changes on Binding of Oligosaccharides to Murine Immunoglobulin A Myeloma Proteins

Biochemistry ◽  
1973 ◽  
Vol 12 (25) ◽  
pp. 5224-5224
Author(s):  
M. Jolley ◽  
S. Rudikoff ◽  
Michael Potter ◽  
C. P. Glaudermans
Keyword(s):  
Immunoglobulin A ◽  
Spectral Changes ◽  
Myeloma Proteins

Carboxy-terminal structure of the α chain of human IgA [immunoglobulin A] myeloma proteins

Biochemistry ◽  
1971 ◽  
Vol 10 (10) ◽  
pp. 1808-1812 ◽  
Author(s):  
James W. Prahl ◽  
Carlos A. Abel ◽  
Howard M. Grey
Keyword(s):  
Immunoglobulin A ◽  
Myeloma Proteins ◽  
Α Chain ◽  
Terminal Structure ◽  
Carboxy Terminal

scholarly journals Comparison of the dimensions of the combining sites of the dinitrophenyl-binding immunoglobulin A myeloma proteins MOPC 315, MOPC 460 and XRPC 25 by spin-label mapping

1977 ◽  
Vol 165 (2) ◽  
pp. 199-206 ◽  
Author(s):  
Keith J. Willan ◽  
Derek Marsh ◽  
Christopher A. Sunderland ◽  
Brian J. Sutton ◽  
Simon Wain-Hobson ◽  
...  
Keyword(s):  
Binding Sites ◽  
Immunoglobulin A ◽  
Binding Activity ◽  
The Other ◽  
Spin Labels ◽  
Metal Site ◽  
Myeloma Proteins ◽  
Spin Resonance ◽  
Mouse Immunoglobulin ◽  
Positively Charged

The mouse immunoglobulin A myeloma proteins MOPC 315, MOPC 460 and XRPC 25 all possess dinitrophenyl (Dnp)-binding activity. Differences in specificities were shown by measuring the affinities of a variety of haptens. By using a series of Dnp-spin-labelled haptens, the dimensions of the binding sites of the three myeloma proteins were compared by the method described for protein MOPC 315 [Sutton, Gettins, Givol, Marsh, Wain-Hobson, Willan & Dwek (1977) Biochem. J.165, 177–197]. The dinitrophenyl ring is rigidly held in all three sites. The depths of the sites are all 1.1–1.2nm, but there are differences in the lateral dimensions at the entrance to the sites. For protein XRPC 25 these dimensions are 0.75nm×0.8nm, which may be compared with 0.85nm×1.1nm for protein MOPC 315 and ≥1.0nm×1.1nm for protein MOPC 460. The site in protein MOPC 460 is more symmetrical with respect to the plane of the dinitrophenyl ring than in either of the other two myeloma proteins and also allows greater penetration of solvent. In protein XRPC 25 a positively charged residue was located at the entrance to the site, similarly positioned to that reported for protein MOPC 315 [Sutton, Gettins, Givol, Marsh, Wain-Hobson, Willan & Dwek (1977) Biochem. J.165, 177–197]. All three proteins possess lanthanide-binding sites, but only in protein MOPC 315 is there antagonism between lanthanide and hapten binding. However, the effects of the diamagnetic La(III) on the electron-spin-resonance spectra of bound Dnp spin labels in both proteins MOPC 460 and XRPC 25 suggest an interaction between the two sites. Comparison of this effect with that caused by the addition of the paramagnetic Gd(III) enables the distance between the lanthanide- and hapten-binding sites to be calculated. In both proteins MOPC 460 and MOPC 315 the metal site is approx. 1.0nm from the nitroxide moiety of the spin-labelled hapten, but in protein XRPC 25 this distance is at least 2.0nm.

scholarly journals Resonance Raman-spectroscopic studies of the hapten features involved in the binding of 2,4-dinitrophenyl haptens by the mouse myeloma proteins MOPC 315 and MOPC 460

1978 ◽  
Vol 175 (2) ◽  
pp. 727-735 ◽  
Author(s):  
K Kumar ◽  
D J Phelps ◽  
P R Carey ◽  
N M Young
Keyword(s):  
Binding Sites ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Immunoglobulin A ◽  
Spectroscopic Studies ◽  
Side Chain ◽  
Raman Spectroscopic ◽  
Myeloma Proteins ◽  
Resonance Raman Spectra ◽  
Mouse Myeloma

The binding of four dinitrophenyl haptens to the mouse myeloma proteins MOPC 315 IgA (immunoglobulin A) and MOPC 460IgA was studied by resonance Raman spectroscopy. Isotopic substitution with 15N and 2H was used to assign features in the resonance Raman spectra of the free haptens. Changes in each of these features on binding to the proteins could then be attributed to interactions of the proteins' binding sites with either the p-NO2 or the o-NO2/amine regions of the haptens. The interactions between a given hapten and MOPC 315 IgA are often quite distinct from those between the same hapten and MOPC 460 IgA. Moreover, for both antibodies the nature of the R side chain in a Dnp-NHR (Dnp, 2,4-dinitrophenyl) compound appears to modify the interactions between the Dnp chromophore and the protein. Thus, with the haptens studied, there is no unique set of contacts between the Dnp group and the binding site. The contacts expected between epsilon-2,4-dinitrophenyl-L-lysine and the site on MOPC 315 IgA, on the basis of a recent model for this site [Dwek, Wain-Hobson, Dower, Gettins, Sutton, Perkins & Givol (1977) Nature (London) 266, 31–37] were not detected. However, the contacts between this hapten and the site on MOPC 460 IgA were closer to those predicted by the model for MOPC 315 IgA.

scholarly journals Studies on J-chain biosynthesis in tumours producing immunoglobulins in NZB mice

1976 ◽  
Vol 156 (3) ◽  
pp. 681-684 ◽  
Author(s):  
H Kaji
Keyword(s):  
Immunoglobulin G ◽  
Immunoglobulin A ◽  
J Chain ◽  
Myeloma Proteins ◽  
Mouse Myeloma

Investigation of biosynthesis of J chain in plasmacytomas induced in NZB mice revealed that this protein was not only synthesized in the cells that produce polymer immunoglobulin A but also in those that produce immunoglobulin G monomer. It was also found that protein similar to J chain of BALB/c-mice was associated with polymer immunoglobulin A but not with immunoglobulin G of NZB mouse myeloma proteins.

Lactate Dehydrogenase-Linked Immunoglobulin A K in a Patient with Nonimmune Chronic Idiopathic Neutropenia

2006 ◽  
Vol 12 (3) ◽  
pp. 148-151
Author(s):  
Noriko Ihara ◽  
Ikuo Murohashi ◽  
Masako Itho ◽  
Ikuo Amino ◽  
Katsuhiko Yoshida ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Immunoglobulin A ◽  
Chronic Idiopathic Neutropenia

The effect of probiotics on the level of immunoglobulin A in women with violation of the biocenosis of the vagina

2017 ◽  
pp. 85-88
Author(s):  
O.I. Ostapenko ◽  
◽  
V.P. Kvashenko ◽  
I.K. Akimova ◽  
I.N. Nosova ◽  
...  
Keyword(s):  
Immunoglobulin A ◽  
Lactobacillus Rhamnosus ◽  
Test System ◽  
Main Group ◽  
Reproductive Age ◽  
Local Action ◽  
Serum Immunoglobulin ◽  
Control Group ◽  
Local Immunity ◽  
Serum Iga

The objective: the study of immunomodulatory effects of a probiotic, which contains lyophilized Lactobacillus (Lactobacillus rhamnosus) – 13 mg (2,0ґ109 CFU) and lyophilized bifidobacteria (Bifidobacterium lactis) – 4 mg (2,0ґ109 CFU) the level of serum immunoglobulin IgA as a marker of local immunity in the plasma of women of reproductive age with the violation of the biocenosis of the vagina. Patients and methods. The study involved 86 patients of reproductive age with the violation of the vaginal biocenosis, which were divided into two groups according to received treatment. A survey was conducted for all patients in both groups: determine the level of serum IgA, measuring pH of vaginal environment and the quantification of lactobacilli and pathogenic flora with the help of test-system «Florotsenoz» before treatment and in 6 weeks after treatment. The state of vaginal microbiocenosis in both groups before treatment was homogeneous. Patients in both groups as therapy at the first stage of treatment received, if necessary antimicrobial therapy depending on the selected flora. In the second stage (restoration of microflora) patient of the main group received systemic probiotic combined with a complex prebiotic local action, patients in the control group, the probiotic localy in the form of the vaginal candles or tablets. Results. The research stated the increasing level of serum IgA in blood plasma of patients of the main group compared to control group at 20%, normalizing the pH of the vaginal environment in the main group in 94% of cases, which indicates an increase of immunity in mucosal. Conclusion. The inclusion of the systemic probiotic in the scheme of treatment of disorders of biocenosis of the vagina system enhances the increasing of immunity of the mucous membranes, and the vaginal tablets prebiotic of local action restores the own normal microflora of the vagina. Key words: serum immunoglobulin A, local immunity, vaginal dysbiosis, probiotics, prebiotics, vaginal microbiocenosis, the pH of the vaginal environment.

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