Control of the action of Vibrio cholerae sialidase on mammalian brain gangliosides by ionic strength

Biochemistry ◽  
1973 ◽  
Vol 12 (10) ◽  
pp. 1858-1861 ◽  
Author(s):  
Vaskresenija Lipovac ◽  
Norman Barton ◽  
Abraham Rosenberg
Keyword(s):  
Ionic Strength ◽  
Vibrio Cholerae ◽  
Mammalian Brain ◽  
Brain Gangliosides ◽  
Vibrio Cholerae Sialidase

scholarly journals Kinetics of Vibrio cholerae sialidase action on gangliosidic substrates at different supramolecular-organizational levels

1982 ◽  
Vol 203 (3) ◽  
pp. 735-742 ◽  
Author(s):  
Bruno Venerando ◽  
Benvenuto Cestaro ◽  
Amelia Fiorilli ◽  
Riccardo Ghidoni ◽  
Augusto Preti ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Vibrio Cholerae ◽  
Bovine Serum ◽  
Mixed Micelles ◽  
Molar Ratio ◽  
Free Carbohydrates ◽  
Triton X ◽  
Kinetics Of ◽  
Vibrio Cholerae Sialidase

Gd1a, Gd1b and Gt1b gangliosides were dispersed in the following membrane-mimicking systems: (a) homogeneous micelles; (b) mixed micelles with Gm1 ganglioside (which is resistant to the enzyme action), Triton X-100 or bovine serum albumin; (c) small unilamellar vesicles of egg phosphatidylcholine. The effect of dispersion on sialic acid release by Vibrio cholerae sialidase was studied. As reference substrates freely interacting with the enzyme the lipid-free carbohydrates of Gd1a and 3′-sialosyl-lactose were employed. The apparent Vmax. of the enzyme was, with all the gangliosides, dependent on the type of ganglioside dispersion. It was lowest for homogeneous micelles and mixed micelles with ganglioside Gm1, and increased about 6-fold for ganglioside/bovine serum albumin lipoprotein micelles, 15-fold for mixed-ganglioside/Triton X-100 micelles (optimal molar ratio 1:7.5) and 30-fold for phosphatidylcholine vesicles containing 2.5 mol% ganglioside (this proportion was optimal for enzyme activity on the vesicles). For ganglioside Gd1a, the activity on Triton X-100 mixed micelles and on mixed vesicles was even greater (3- and 6-fold respectively) than that displayed on Gd1a lipid-free carbohydrate. With each of the used gangliosides the apparent Km values were very similar values for homogeneous micelles and vesicular dispersions, but showed marked increases for Triton X-100 mixed micelles, approaching the values exhibited by reference oligosaccharides. Triton X-100 micelles and phosphatidylcholine vesicles did not appreciably alter the kinetics of sialidase action on 3′-sialosyl-lactose and on Gd1a lipid-free carbohydrate, indicating that the above effects are dependent on the intrinsic characteristics of the membrane-like systems containing gangliosides.

The design, synthesis and biological evaluation of neuraminic acid-based probes of Vibrio cholerae sialidase

2000 ◽  
Vol 11 (1) ◽  
pp. 53-73 ◽  
Author(s):  
Jennifer C Wilson ◽  
Robin J Thomson ◽  
Jeffrey C Dyason ◽  
Pas Florio ◽  
Kaylene J Quelch ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Biological Evaluation ◽  
Neuraminic Acid ◽  
Design Synthesis ◽  
Synthesis And Biological Evaluation ◽  
Vibrio Cholerae Sialidase

Modelling, synthesis and biological evaluation of novel glucuronide-based probes of Vibrio cholerae sialidase

2006 ◽  
Vol 14 (5) ◽  
pp. 1518-1537 ◽  
Author(s):  
Maretta C. Mann ◽  
Robin J. Thomson ◽  
Jeffrey C. Dyason ◽  
Sarah McAtamney ◽  
Mark von Itzstein
Keyword(s):  
Vibrio Cholerae ◽  
Biological Evaluation ◽  
Synthesis And Biological Evaluation ◽  
Vibrio Cholerae Sialidase

scholarly journals The Study of Viability of Vibrio cholerae Strains in Low Ionic Strength Aquatic Environment

2019 ◽  
Author(s):  
Subha Sankar Paul ◽  
Eizo Takahashi ◽  
Goutam Chowdhury ◽  
Shin-ichi Miyoshi ◽  
Asish K. Mukhopadhyay ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Vibrio Cholerae ◽  
Aquatic Environment ◽  
Detection Rate ◽  
Environmental Water ◽  
Toxin Gene ◽  
Aquatic Medium ◽  
Vibrio Cholerae O1 ◽  
The Difference ◽  
Low Ionic Strength

It has been regarded that Vibrio cholerae O1 inhabit in environmental water. As many cholera patients emerge in Kolkata, it has been thought that V. cholerae O1 is easily detected in environmental water in Kolkata. However, the detection of V. cholerae O1 is rare, though other V. cholerae (NAG Vibrio) is constantly detected. To clear the reason for the difference of the detection rate of two Vibrios, we examined the viability of V. cholera O1 and NAG Vibrios in low ionic strength aquatic medium. We observed greater declining viability of V. cholerae O1 possessing cholera toxin gene (ctx) in low ionic strength solution, but the decline of NAG Vibrios non-possessing ctx is small. To evaluate the concerning of ctx in the viability, we examined the viabilities of V. cholerae O1which do not possess ctx and NAG Vibrios possessing ctx under the same condition. The result indicated that the existence of the ctx induces the decrease the viability of the host in low ionic strength solution. The decrease observed in this experiment might relate with the low detection of V. cholerae O1 possessing ctx in environmental water, though NAG Vibrio is constantly detected.

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