Isolation and properties of two biologically active fragments from limited tryptic hydrolysis of bovine and ovine pituitary growth hormones

Biochemistry ◽  
1974 ◽  
Vol 13 (26) ◽  
pp. 5408-5415 ◽  
Author(s):  
Laszlo Graf ◽  
Choh Hao Li
Keyword(s):  
Growth Hormones ◽  
Biologically Active ◽  
Active Fragments ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis

scholarly journals Kinetic Modeling of the Formation of Peptide Nanoparticles by Tryptic Hydrolysis of Β-Casein

INEOS OPEN ◽  
2021 ◽  
Author(s):  
A. V. Golovanov ◽  
◽  
M. M. Vorob'ev ◽  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Chemical Kinetics ◽  
Kinetic Modeling ◽  
Kinetic Models ◽  
Enzyme Concentration ◽  
Biologically Active ◽  
Peptide Nanoparticles ◽  
Hydrolysis Of ◽  
Controlled Hydrolysis ◽  
Tryptic Hydrolysis

Peptide nanoparticles obtained by the enzymatic hydrolysis of proteins are new biocompatible nontoxic systems for the transport of biologically active compounds. The formation of peptide nanoparticles from -casein during its controlled hydrolysis by trypsin was modeled by the methods of chemical kinetics. The product of the time of nanoparticle formation (tmax) by the enzyme concentration (E0) was analyzed in different kinetic models. The dependence of tmax∙E0 on the enzyme concentration was used to estimate the contribution of the enzymatic and non-enzymatic stages to the formation of nanoparticles.

scholarly journals Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach

Antibiotics ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1211
Author(s):  
Joe-Hui Ong ◽  
Wey-Lim Wong ◽  
Fai-Chu Wong ◽  
Tsun-Thai Chai
Keyword(s):  
Complex Formation ◽  
In Silico ◽  
Vibrio Parahaemolyticus ◽  
Water Solubility ◽  
Toxin Binding ◽  
Binding Peptides ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis ◽  
Dual Target

Acute hepatopancreatic necrosis disease (AHPND), caused by PirAvp- and PirBvp-releasing Vibrio parahaemolyticus strains, has resulted in massive mortality in shrimp aquaculture. Excessive use of antibiotics for AHPND management has led to antibiotic resistance, highlighting the urgency to search for alternatives. Using an in silico approach, we aimed to discover PirAvp/PirBvp-binding peptides from oilseed meals as alternatives to antibiotics. To search for peptides that remain intact in the shrimp digestive tract, and therefore would be available for toxin binding, we focused on peptides released from tryptic hydrolysis of 37 major proteins from seeds of hemp, pumpkin, rape, sesame, and sunflower. This yielded 809 peptides. Further screening led to 24 peptides predicted as being non-toxic to shrimp, fish, and humans, with thermal stability and low water solubility. Molecular docking on the 24 peptides revealed six dual-target peptides capable of binding to key regions responsible for complex formation on both PirAvp and PirBvp. The peptides (ISYVVQGMGISGR, LTFVVHGHALMGK, QSLGVPPQLGNACNLDNLDVLQPTETIK, ISTINSQTLPILSQLR, PQFLVGASSILR, and VQVVNHMGQK) are 1139–2977 Da in mass and 10–28 residues in length. Such peptides are potential candidates for the future development of peptide-based anti-AHPND agents which potentially mitigate V. parahaemolyticus pathogenesis by intercepting PirAvp/PirBvp complex formation.

scholarly journals SECONDARY METABOLITES WITH ANTIBIOTIC ACTIVITY OF MARINE ACTINOBACTERIA

2021 ◽  
pp. 28-43
Author(s):  
K. S. Potapenko ◽  
N. V. Korotaieva ◽  
V. О. Ivanytsia
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Bioactive Compounds ◽  
Current Literature ◽  
Antibiotic Activity ◽  
Growth Hormones ◽  
Biologically Active ◽  
Marine Actinobacteria ◽  
Biotechnological Potential ◽  
Plant Growth Hormones

Marine actinobacteria are active producers and an unused rich source of various biologically active secondary metabolites, such as antibiotics, antitumor, antiviral and antiinflammatory compounds, biopesticides, plant growth hormones, pigments, enzymes, enzyme inhibitors.In this review describes data from current literature sources for the period from 2017 to 2021 about various bioactive compounds that produce marine actinobacteria, their antibiotic activity and biotechnological potential, the main groups of secondary metabolites and their producers.

Limited tryptic hydrolysis of faba bean legumin: Conformational stability of legumin-T

Nahrung/Food ◽  
1998 ◽  
Vol 42 (03-04) ◽  
pp. 160-161
Author(s):  
Th. Henning ◽  
S. Dudek ◽  
K.D. Schwenke ◽  
A.N. Danilenko ◽  
E.E. Braudo
Keyword(s):  
Faba Bean ◽  
Conformational Stability ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis

scholarly journals Synthesis of Kisspeptin-Mimicking Fragments and Investigation of their Skin Anti-Aging Effects

2020 ◽  
Vol 21 (22) ◽  
pp. 8439
Author(s):  
Kyung-Eun Lee ◽  
Sugyeong Jeong ◽  
Seok Kyun Yun ◽  
Seoyeon Kyung ◽  
Abadie Sophie ◽  
...  
Keyword(s):  
Skin Aging ◽  
Dermal Fibroblasts ◽  
Biologically Active ◽  
Type I ◽  
Aging Effects ◽  
Type I Procollagen ◽  
Reproductive Axis ◽  
Stress Related Genes ◽  
Active Fragments ◽  
Aging Models

In recent years, a number of active materials have been developed to provide anti-aging benefits for skin and, among them, peptides have been considered the most promising candidate due to their remarkable and long-lasting anti-wrinkle activity. Recent studies have begun to elucidate the relationship between the secretion of emotion-related hormones and skin aging. Kisspeptin, a neuropeptide encoded by the KISS1 gene, has gained attention in reproductive endocrinology since it stimulates the reproductive axis in the hypothalamus; however, the effects of Kisspeptin on skin have not been studied yet. In this study, we synthesized Kisspeptin-10 and Kisspeptin-E, which are biologically active fragments, to mimic the action of Kisspeptin. Next, we demonstrated the anti-aging effects of the Kisspeptin-mimicking fragments using UV-induced skin aging models, such as UV-induced human dermal fibroblasts (Hs68) and human skin explants. Kisspeptin-E suppressed UV-induced 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) stimulation leading to a regulation of skin aging related genes, including type I procollagen, matrix metalloproteinases-1 (MMP-1), interleukin-6 (IL-6), and IL-8, and rescued the skin integrity. Taken together, these results suggest that Kisspeptin-E could be useful to improve UV-induced skin aging by modulating expression of stress related genes, such as 11β-HSD1.

Kinetics of the Tryptic Hydrolysis of the Oxidized B Chain of Bovine Insulin*

Biochemistry ◽  
1967 ◽  
Vol 6 (1) ◽  
pp. 215-224 ◽  
Author(s):  
Su-Sun Wang ◽  
Frederick H. Carpenter
Keyword(s):  
Bovine Insulin ◽  
Kinetics Of ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis

Peptides obtained by tryptic hydrolysis of bovine β-lactoglobulin induce specific oral tolerance in mice

2000 ◽  
Vol 105 (3) ◽  
pp. 514-521 ◽  
Author(s):  
Sophie Pecquet ◽  
Lionel Bovetto ◽  
Françoise Maynard ◽  
Rodolphe Fritsché
Keyword(s):  
Oral Tolerance ◽  
Β Lactoglobulin ◽  
Hydrolysis Of ◽  
Tryptic Hydrolysis
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