Fast relaxation processes in a protein revealed by the decay kinetics of tryptophan fluorescence

Biochemistry ◽  
1974 ◽  
Vol 13 (25) ◽  
pp. 5170-5178 ◽  
Author(s):  
A. Grinvald ◽  
I. Z. Steinberg
Keyword(s):  
Tryptophan Fluorescence ◽  
Relaxation Processes ◽  
Decay Kinetics ◽  
Fast Relaxation ◽  
Kinetics Of

scholarly journals Decay of Metastable State with Account of Agglomeration and Relaxation Processes

2016 ◽  
Vol 2016 ◽  
pp. 1-9
Author(s):  
Victor Kurasov
Keyword(s):  
Metastable State ◽  
Metastable Phase ◽  
Theoretical Description ◽  
Relaxation Processes ◽  
Decay Kinetics ◽  
Kinetics Of

Theoretical description of the metastable phase decay kinetics in the presence of specific connections between the embryos of small sizes has been given. The theory of the decay kinetics in the presence of relaxation processes is constructed in analytical manner. The m-mers nucleation is investigated and the global kinetics of decay is also constructed in this case analytically.

Spectra and decay kinetics of trimethyldioxetane chemiluminescence

1975 ◽  
Vol 34 (2) ◽  
pp. 326-331 ◽  
Author(s):  
Irene Simo ◽  
Joachim Stauff
Keyword(s):  
Decay Kinetics ◽  
Kinetics Of

Picosecond decay kinetics of CdS luminescence studied by a streak camera

1979 ◽  
Vol 31 (4) ◽  
pp. 253-256 ◽  
Author(s):  
T. Kobayashi ◽  
Y. Segawa ◽  
S. Namba
Keyword(s):  
Streak Camera ◽  
Decay Kinetics ◽  
Kinetics Of

Decay Kinetics of Electron Emission from MgO Films in AC Plasma Display Panels

2008 ◽  
Vol 1 ◽  
pp. 091203 ◽  
Author(s):  
Takeru Okada ◽  
Takashi Furutani ◽  
Toshihiro Yoshioka
Keyword(s):  
Electron Emission ◽  
Decay Kinetics ◽  
Plasma Display Panels ◽  
Plasma Display ◽  
Kinetics Of

The tryptophan fluorescence lifetime puzzle. A study of decay times in aqueous solution as a function of pH and buffer composition

1981 ◽  
Vol 59 (7) ◽  
pp. 1037-1044 ◽  
Author(s):  
Eva Gudgin ◽  
Ricardo Lopez-Delgado ◽  
William R. Ware
Keyword(s):  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Low Ph ◽  
Decay Kinetics ◽  
Decay Component ◽  
Diffusion Controlled ◽  
Double Exponential ◽  
Decay Times ◽  
Fluorescence Decay Kinetics ◽  
Buffer Composition

Tryptophan fluorescence decay kinetics have been systematically investigated in aqueous solutions as a function of pH as well as in a variety of buffer solutions. Below pH 7.0, the decay appears to be double exponential with a subnanosecond component confirming the previous findings of Rayner and Szabo (3). In the low pH region, where the proton concentration becomes kinetically significant, tryptophan fluorescence is collisionally quenched by [H+] with diffusion controlled rate and no experimental evidence is found regarding the appearance at low pH of a new tryptophan molecular species, namely the cationic form. At pH ≥ 7.0, the decay becomes triple-exponential with the appearance of a long component whose contribution to the total emission intensity increases rapidly with increasing pH at the expense of the other two. Lifetimes and relative intensities of each decay component depend in a complex way on pH and on the buffer chemical composition.

scholarly journals Temporal patterns of damage and decay kinetics of DNA retrieved from plant herbarium specimens

2015 ◽  
Author(s):  
Clemens L Weiß ◽  
Verena J Schuenemann ◽  
Jane Devos ◽  
Gautam Shirsekar ◽  
Ella Reiter ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Dna Degradation ◽  
Herbarium Specimens ◽  
Decay Kinetics ◽  
Strand Breaks ◽  
Last 300 Years ◽  
Computational Procedures ◽  
Breaking Points ◽  
Kinetics Of ◽  
Historical Nature

Herbaria archive a record of changes of worldwide plant biodiversity harboring millions of specimens that contain DNA suitable for genome sequencing. To profit from this resource, it is fundamental to understand in detail the process of DNA degradation in herbarium specimens. We investigated patterns of DNA fragmentation -length and base composition at breaking points-, and nucleotide misincorporation by analyzing 86 herbarium samples spanning the last 300 years using Illumina shot-gun sequencing. We found an exponential decay relationship between DNA fragmentation and time, and estimated a per nucleotide fragmentation rate of 1.66 x 10-4 per year, which is ten times faster than the rate estimated for fossilized bones. Additionally, we found that strand breaks occur specially before purines, and that depurination-driven DNA breakage occurs constantly through time and can to a great extent explain decreasing fragment length over time. Similar of what has been found analyzing ancient DNA from bones, we found a strong correlation between the deamination-driven accumulation of cytosine (C) to thymine (T) substitutions and time, which reinforces the importance of substitution patterns to authenticate the ancient/historical nature of DNA fragments. Accurate estimations of DNA degradation through time will allow informed decisions about laboratory and computational procedures to take advantage of the vast collection of worldwide herbarium specimens.

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