Mononucleotides in aqueous solution. Proton magnetic resonance studies of amino groups

Biochemistry ◽  
1974 ◽  
Vol 13 (22) ◽  
pp. 4616-4622 ◽  
Author(s):  
Matthew Raszka
Keyword(s):  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Amino Groups ◽  
Magnetic Resonance Studies

Ribo-2′-deoxyribo hybrid dinucleoside monophosphates. Proton magnetic resonance studies of 3′,5′- and 2′,5′-uridylyl-2′-deoxythymidine

1978 ◽  
Vol 56 (4) ◽  
pp. 522-529 ◽  
Author(s):  
James Peeling ◽  
Frank E. Hruska ◽  
Peter C. Loewen
Keyword(s):  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Molecular Conformation ◽  
The Other ◽  
Magnetic Resonance Studies ◽  
Dinucleoside Monophosphates ◽  
Standard Techniques

This 1Hmr study initiates our examination of the conformations of dinucleoside monophosphates possessing ribo 2′- or 3′-nucleotidyl units linked to 5′-nucleotidyl units possessing the 2′-deoxyribo sugar. The syntheses of uridylyl-3′,5′-2′-deoxythymidine (3′,5′-UpdT) and its 2′,5′-isomer, 2′,5′-UpdT, were carried out with standard techniques. The 1Hmr data were obtained at frequencies up to 270 MHz and used to derive the dominant conformation of the dimers in aqueous solution. Comparison with data for the component mononucleotides reveals that dimerization does not lead to drastic changes in the molecular conformation. Literature data for dimers possessing only the ribo sugar (3′,5′-UpU) and the 2′-deoxyribo sugar (3′,5′-d(TpT)) are also presented. The results indicate that, at least for our dipyrimidine dimers, the molecular conformation of a particular fragment is not critically dependent on the nature (ribo or 2′-deoxyribo) of the other nucleotide unit.

Proton Magnetic Resonance Studies of 2′-Deoxythymidine, its 3′- and 5′-Monophosphates and 2′-Deoxythymidylyl-(3′, 5′)-2′ -deoxythymidine in Aqueous Solution

1974 ◽  
Vol 52 (19) ◽  
pp. 3353-3366 ◽  
Author(s):  
Donald J. Wood ◽  
Frank E. Hruska ◽  
Kelvin K. Ogilvie
Keyword(s):  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Chemical Shift ◽  
Proton Magnetic Resonance ◽  
Coupling Constant ◽  
Experimental Conditions ◽  
Magnetic Resonance Studies ◽  
Spectral Bands ◽  
Conformational Properties

Proton magnetic resonance studies of 2′-deoxythymidine, its 3′ and 5′-monophosphates and the dideoxynucleoside monophosphate, 2′-deoxythymidylyl-(3′,5′)-2′-deoxythymidine are described. Assignment of the spectral bands are carried out and the derived chemical shift and coupling constant data are discussed in terms of the conformational properties of these molecules. The main conclusion reached is that 3′- and 5′-phosphorylation as well as incorporation into the dinucleoside monophosphate at either the 3′- or 5′-terminus has only slight effects on the conformation of thymidine under the experimental conditions employed.

Proton Magnetic Resonance Studies of Dinucleotide Conformation in Aqueous Solution. 2′-Deoxythymidylyl-(3′,5′)-2′-deoxy-3′-thymidylate, d(TpTp)

1975 ◽  
Vol 53 (18) ◽  
pp. 2781-2790 ◽  
Author(s):  
Donald J. Wood ◽  
Kelvin K. Ogilvie ◽  
Frank E. Hruska
Keyword(s):  
Aqueous Solution ◽  
Spectral Analysis ◽  
Magnetic Resonance ◽  
Proton Magnetic Resonance ◽  
Chemical Shifts ◽  
Coupling Constants ◽  
Gauche Conformation ◽  
Magnetic Resonance Studies ◽  
Important Observation ◽  
Conformational Properties

Proton magnetic resonance studies of 2′-deoxythymidine, its 3′-and 5′-monophosphate, its 3′,5′-diphosphate, and the dimer molecules d(TpT) and d(TpTp) in aqueous solution are de scribed. Spectral analysis yields 1H–1H and 1H–31P coupling constants and 1H chemical shifts which can be discussed in terms of the conformational properties of the nucleoside fragments. An important observation is the apparent phosphate–phosphate interaction in a 3′,5′-diphosphate fragment which stabilizes the gauche-gauche conformation about the C4′—C5′ bond.

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