Respiration-linked calcium ion uptake by flight muscle mitochondria from the blowfly Sarcophaga bullata

Biochemistry ◽  
1974 ◽  
Vol 13 (19) ◽  
pp. 4014-4018 ◽  
Author(s):  
Hartmut Wohlrab
Keyword(s):  
Calcium Ion ◽  
Flight Muscle ◽  
Ion Uptake ◽  
Muscle Mitochondria ◽  
Sarcophaga Bullata

scholarly journals The effect of the coupled oxidation of substrate on the permeability of blowfly flight-muscle mitochondria to potassium and other cations

1972 ◽  
Vol 126 (3) ◽  
pp. 689-700 ◽  
Author(s):  
R. G. Hansford ◽  
A. L. Lehninger
Keyword(s):  
Light Scattering ◽  
Flight Muscle ◽  
Unit Area ◽  
Membrane Surface ◽  
Mitochondrial Matrix ◽  
Exposed Area ◽  
Muscle Mitochondria ◽  
Area 5 ◽  
Choline Phosphate ◽  
Permeant Anion

1. Blowfly flight-muscle mitochondria respiring in the absence of phosphate acceptor (i.e. in state 4) take up greater amounts of K+, Na+, choline, phosphate and Cl-(but less NH4+) than non-respiring control mitochondria. 2. Uptake of cations is accompanied by an increase in the volume of the mitochondrial matrix, determined with the use of [14C]-sucrose and3H2O. The osmolarity of the salt solution taken up was approximately that of the suspending medium. 3. The [14C]sucrose-inaccessible space decreased with increasing osmolarity of potassium chloride in the suspending medium, confirming that the blowfly mitochondrion behaves as an osmometer. 4. Light-scattering studies showed that both respiratory substrate and a permeant anion such as phosphate or acetate are required for rapid and massive entry of K+, which occurs in an electrophoretic process rather than in exchange for H+. The increase in permeability to K+and other cations is probably the result of a large increase in the exposed area of inner membrane surface in these mitochondria, with no intrinsic increase in the permeability per unit area. 5. No increase in permeability to K+and other cations occurs during phosphorylation of ADP in state 3 respiration.

scholarly journals Histochemical localization of trehalase activity in dorsal flight muscle of the flesh fly Sarcophaga bullata with light and electron microscopy.

1975 ◽  
Vol 23 (11) ◽  
pp. 800-807 ◽  
Author(s):  
P L Chang ◽  
P E Morrison
Keyword(s):  
Electron Microscopy ◽  
Incubation Medium ◽  
Mitochondrial Membrane ◽  
Flight Muscle ◽  
Light And Electron Microscopy ◽  
Inner Mitochondrial Membrane ◽  
Electron Microscopic ◽  
Flesh Fly ◽  
Trehalase Activity ◽  
Sarcophaga Bullata

Trehalase activity in flight muscle of the flesh fly Sacrophaga bullata is detected histochemically at light- and electron-microscopic levels by using diaminobenzidine, glucose oxidase and peroxidase in the incubation medium. The association of trehalase activity with the inner mitochondrial membrane is confirmed. Biochemical assay shows that about 50% of the initial total trehalase activity is lost from the tissue during the histochemical processing and about 50% remains for histochemical detection.

Accelerated calcium ion uptake in murine thymocytes induced by concanavalin A

1977 ◽  
Vol 93 (1) ◽  
pp. 153-160 ◽  
Author(s):  
Keiko Ozato ◽  
Leaf Huang ◽  
James D. Ebert
Keyword(s):  
Concanavalin A ◽  
Calcium Ion ◽  
Ion Uptake

scholarly journals Effect of calcium ion uptake onCandida albicansmorphology

1991 ◽  
Vol 77 (2-3) ◽  
pp. 187-194 ◽  
Author(s):  
Ann R. Holmes ◽  
Richard D. Cannon ◽  
Maxwell G. Shepherd
Keyword(s):  
Calcium Ion ◽  
Ion Uptake
Sign in / Sign up

Export Citation Format

Share Document