Conformation of procine D-amino acid oxidase as studied by protein fluorescence and optical rotatory dispersion

Biochemistry ◽  
1974 ◽  
Vol 13 (5) ◽  
pp. 893-899 ◽  
Author(s):  
Shiao-Chun Tu ◽  
Donald B. McCormick
Keyword(s):  
Amino Acid ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Acid Oxidase ◽  
Optical Rotatory ◽  
Protein Fluorescence ◽  
Amino Acid Oxidase

scholarly journals Optical Rotatory Dispersion and Circular Dichroism of Amino Acid Hydantoins

1973 ◽  
Vol 37 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Tateo SUZUKI ◽  
Keiji IGARASHI ◽  
Kieko HASE ◽  
Katura TUZIMURA
Keyword(s):  
Circular Dichroism ◽  
Amino Acid ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Circular Dichroïsm

scholarly journals The isolation and properties of experimental allergic encephalitogenic protein

1969 ◽  
Vol 111 (5) ◽  
pp. 629-636 ◽  
Author(s):  
F. B. Palmer ◽  
R. M. C. Dawson
Keyword(s):  
Amino Acid ◽  
Secondary Structure ◽  
Gel Filtration ◽  
Small Degree ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Amino Acid Residues ◽  
Optical Rotatory ◽  
Antigenic Activity ◽  
Experimental Allergic

1. Experimental allergic encephalitogenic (EAE) protein was isolated from ox spinal cord by a modification of the method of Martenson & LeBaron (1966). 2. The protein was examined by acrylamide-gel electrophoresis and its amino acid composition determined. 3. Sedimentation-velocity runs in the ultracentrifuge indicate a molecular weight of about 15100 at pH7·8, and calculation suggests that approx. 137 amino acid residues are present per molecule. 4. Gel-filtration and diffusion studies suggest that the protein is non-globular. 5. Optical-rotatory-dispersion measurements show the protein to have no helical secondary structure even at pH values near to the isoelectric point. In the presence of triphosphoinositide, changes in the optical rotatory dispersion of the protein could be interpreted to mean that it develops a small degree of secondary structure. 6. On treatment with cyanogen bromide the experimental allergic encephalitogenic protein is split into at least two fragments, the larger of which is only about 12% smaller than the parent protein and is antigenically active, and the smaller of which is devoid of antigenic activity.

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