Structure of ovotransferrin. I. Isolation and characterization of cyanogen bromide fragments. Reevaluation of the primary structure

Biochemistry ◽  
1974 ◽  
Vol 13 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Dean Tsao ◽  
Parviz Azari ◽  
Jerry L. Phillips
Keyword(s):  
Primary Structure ◽  
Cyanogen Bromide ◽  
Isolation And Characterization

scholarly journals The primary structure of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum. Peptides derived from digestion with cyanogen bromide, and the sequences of three long extramembranous segments

1980 ◽  
Vol 187 (3) ◽  
pp. 591-616 ◽  
Author(s):  
G Allen ◽  
B J Trinnaman ◽  
N M Green
Keyword(s):  
Sarcoplasmic Reticulum ◽  
Primary Structure ◽  
Calcium Ion ◽  
Adenosine Triphosphatase ◽  
Cyanogen Bromide ◽  
Secondary Structures ◽  
British Library ◽  
Isolation And Characterization ◽  
Tryptophan Residues

The isolation and characterization of the soluble peptides from the CNBr digest of the calcium ion-transporting adenosine triphosphatase protein of rabbit skeletal sarcoplasmic reticulum are described. The 562 unique residues of the protein were placed in sequences. The remaining part of the protein (about 500 residues) yielded long hydrophobic sequences that contained all but one of the tryptophan residues of the protein and that were probably derived largely from the intramembranous parts of the protein. Three long stretches of primary structure, constituting half of the protein, have been reconstructed from the information presented here together with the sequences found in peptides from other digests of the protein. The secondary structures of these sequences have been predicted. A model for the primary structure of the protein is presented and the implications discussed. Details of the isolation of peptides are contained in Supplementary Publication, SUP 50105 (29 pages), which has been deposited with the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1978) 169, 5.

Structure of ovotransferrin. II. Isolation and characterization of a specific iron-binding fragment after cyanogen bromide cleavage

Biochemistry ◽  
1974 ◽  
Vol 13 (3) ◽  
pp. 403-407 ◽  
Author(s):  
Dean Tsao ◽  
Dan H. Morris ◽  
Parviz Azari ◽  
Robert P. Tengerdy ◽  
Jerry L. Phillips
Keyword(s):  
Cyanogen Bromide ◽  
Iron Binding ◽  
Isolation And Characterization

Isolation and characterization of the cyanogen bromide fragments from the high-density apolipoprotein glutamine I

Biochemistry ◽  
1973 ◽  
Vol 12 (20) ◽  
pp. 3866-3871 ◽  
Author(s):  
H. Nordean Baker ◽  
Richard L. Jackson ◽  
Antonio M. Gotto
Keyword(s):  
Cyanogen Bromide ◽  
High Density ◽  
Isolation And Characterization
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