Characterization of an inhibitor causing potassium chloride sensitivity of an RNA polymerase from T4 phage-infected Escherichia coli

Biochemistry ◽  
1975 ◽  
Vol 14 (23) ◽  
pp. 5074-5079 ◽  
Author(s):  
Audrey Stevens ◽  
Joyce C. Rhoton
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Potassium Chloride ◽  
T4 Phage

Precise Characterization of the Epitope Recognized by a Monoclonal Antibody Against Escherichia coli RNA Polymerase

Hybridoma ◽  
2005 ◽  
Vol 24 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Estelle Andre ◽  
Martine Pugniere ◽  
Jaqueline Latouche ◽  
Claude Granier ◽  
Jean-Paul Leonetti
Keyword(s):  
Escherichia Coli ◽  
Monoclonal Antibody ◽  
Rna Polymerase ◽  
Precise Characterization

ELECTROSTATIC PROPERTIES OF PROMOTER RECOGNIZED BYE. COLIRNA POLYMERASE Eσ70

2006 ◽  
Vol 04 (02) ◽  
pp. 455-467 ◽  
Author(s):  
ANATOLY A. SOROKIN ◽  
ALEXANDR A. OSYPOV ◽  
TIMUR R. DZHELYADIN ◽  
PETR M. BESKARAVAINY ◽  
SVETLANA G. KAMZOLOVA
Keyword(s):  
Escherichia Coli ◽  
Comparative Analysis ◽  
Rna Polymerase ◽  
Α Subunit ◽  
Promoter Sequences ◽  
E Coli ◽  
Adp Ribosylation ◽  
Electrostatic Properties ◽  
Differential Recognition ◽  
T4 Phage

A comparative analysis of electrostatic patterns for 359 σ70-specific promoters and 359 nonpromoter regions on electrostatic map of Escherichia coli genome was carried out. It was found that DNA is not a uniformly charged molecule. There are some local inhomogeneities in its electrostatic profile which correlate with promoter sequences. Electrostatic patterns of promoter DNAs can be specified due to the presence of some distinctive motifs which differ for different promoter groups and may be involved as signal elements in differential recognition of various promoters by the enzyme. Some specific electrostatic elements which are responsible for modulating promoter activities due to ADP-ribosylation of RNA polymerase α-subunit were found in far upstream regions of T4 phage early promoters and E. coli ribosomal promoters.

scholarly journals Promoter Interference in a Bacteriophage Lambda Control Region: Effects of a Range of Interpromoter Distances

2000 ◽  
Vol 182 (1) ◽  
pp. 216-220 ◽  
Author(s):  
Michael G. Strainic ◽  
Jennifer J. Sullivan ◽  
Julio Collado-Vides ◽  
Pieter L. deHaseth
Keyword(s):  
Escherichia Coli ◽  
Complex Formation ◽  
Rna Polymerase ◽  
Control Region ◽  
Bacteriophage Lambda ◽  
Detailed Characterization ◽  
Phosphodiester Bonds ◽  
Divergent Promoters ◽  
Polymerase Binding

ABSTRACT The pR and pRM promoters of bacteriophage lambda direct transcription in divergent directions from start sites separated by 83 phosphodiester bonds. We had previously shown that the presence of an RNA polymerase at pR interfered with open complex formation at pRM and that this effect was alleviated by the deletion of 10 bp between the two promoters. Here we present a detailed characterization of the dependence of the interference on the interpromoter distance. It was found that the reduced interference between the two promoters is unique to the 10-bp deletion. The relief of interference was demonstrated to be due to the facilitation of a step subsequent to RNA polymerase binding to the pRM promoter. A model to explain these observations is proposed. A search of known Escherichia coli promoters identified three pairs of divergent promoters with similar separations to those investigated here.

Sign in / Sign up

Export Citation Format

Share Document