DNA synthesis in isolated HeLa cell nuclei. Evidence for in vitro initiation of synthesis of small pieces of DNA and their subsequent ligation

Biochemistry ◽  
1975 ◽  
Vol 14 (19) ◽  
pp. 4233-4237 ◽  
Author(s):  
Hans Krokan ◽  
Lindsay Cooke ◽  
Hans Prydz
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Cell Nuclei

scholarly journals Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

1978 ◽  
Vol 173 (1) ◽  
pp. 309-314 ◽  
Author(s):  
T R Butt ◽  
W M Wood ◽  
E L McKay ◽  
R L P Adams
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Nuclear Dna ◽  
Dna Polymerase Beta ◽  
Cell Nuclei ◽  
High Molecular Weight Dna ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

Effect of 1-β-D-arabinofuranosylcytosine triphosphate on DNA synthesis in isolated HeLa cell nuclei

Biochemistry ◽  
1976 ◽  
Vol 15 (16) ◽  
pp. 3647-3652 ◽  
Author(s):  
Erik Wist ◽  
Hans Krokan ◽  
Hans Prydz
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Cell Nuclei

scholarly journals Effect of drugs on deoxyribonucleic acid synthesis in isolated mammalian cell nuclei. Comparison with partially purified deoxyribonucleic acid polymerases

1979 ◽  
Vol 178 (3) ◽  
pp. 621-626 ◽  
Author(s):  
J F Burke ◽  
P M Duff ◽  
C K Pearson
Keyword(s):  
Dna Synthesis ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Cell Nuclei ◽  
Isolated Nuclei ◽  
Dna Polymerase Alpha ◽  
Polymerase Beta ◽  
Deoxyribonucleic Acid Synthesis

In order to ascertain the identity of the DNA-dependent DNA polymerase responsible for the observed DNA synthesis in nuclei isolated from baby-hamster kidney (BHK-21/C13) cells a comparative study was carried out on the effects of some drugs, reported to influence DNA synthesis, on DNA synthesis catalysed by these nuclei and by partially purified DNA polymerase-alpha and -beta. In all cases DNA synthesis by isolated nuclei and polymerase-alpha was inhibited to similar extents by N-ethylmaleimide, p-hydroxymercuribenzoate, novobiocin, heparin and phosphonoacetic acid; polymerase-beta was much less affected by these compounds. Ethidium bromide inhibited all DNA synthesis to similar extents, although at low concentrations (about 2 microgram/ml) synthesis in isolated nuclei was stimulated. The results are discussed in relation to the proposal that DNA polymerase-alpha catalyses the covalent extension of Okazaki fragments that these nuclei carry out in vitro.

DNA synthesis in HeLa cell nuclei isolated in a non-aqueous medium

1975 ◽  
Vol 395 (4) ◽  
pp. 413-421
Author(s):  
Unni Spaeren ◽  
Knut Schrøder ◽  
Carol Sudbery ◽  
Eirik Bjørklid ◽  
Hans Prydz
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Aqueous Medium ◽  
Cell Nuclei

Effect of cytosol on DNA synthesis in isolated HeLa cell nuclei

1977 ◽  
Vol 75 (2) ◽  
pp. 414-419 ◽  
Author(s):  
Hans Krokan ◽  
Erik Wist ◽  
Hans Prydz
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Cell Nuclei
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