Preparation and properties of three specific active derivatives of ribonuclease A obtained by methylation of methionine residues in 8 M urea

Biochemistry ◽  
1975 ◽  
Vol 14 (15) ◽  
pp. 3476-3481 ◽  
Author(s):  
George R. Stark ◽  
Thomas P. Link
Keyword(s):  
Ribonuclease A ◽  
Methionine Residues ◽  
Derivatives Of

scholarly journals Selective hepatic uptake of synthetic glycoproteins: mannosaminated ribonuclease A dimer and serum albumin.

1979 ◽  
Vol 74 (4) ◽  
pp. 495-509 ◽  
Author(s):  
G Wilson
Keyword(s):  
Serum Albumin ◽  
Reductive Amination ◽  
Hepatic Uptake ◽  
Ribonuclease A ◽  
Liver Uptake ◽  
Lysine Residues ◽  
Minor Site ◽  
Pancreatic Ribonuclease A ◽  
A Minor ◽  
Derivatives Of

The influence of mannose-containing oligosaccharides on the tissue uptake of glycoproteins has been examined with synthetic glycoconjugates. Oligosaccharides obtained from the acetolysis of bakers' yeast mannan have been coupled to the lysine residues of the cross-linked dimer of bovine pancreatic ribonuclease A and of human serum albumin by reductive amination with cyanoborohydride. 14C-labeled derivatives of the two proteins containing two to four mannopyranose residues per 10,000 mol wt were administered intravenously to rats. There was selective (70-80%) uptake of these derivatives by the liver within 10-15 min after injection. A minor site of uptake was the spleen. The extent of hepatic uptake was a function of the number and size of the mannooligosaccharide residues coupled. With the nonglycosaminated derivatives the liver uptake was less than 5%. Related studies have shown that mannose-containing glycoproteins are taken up by both the endothelial and Kupffer cells of the liver; thus, reductive mannosamination may provide a means of directing to these cells proteins of potential therapeutic interest.

Crystal structure of two covalent nucleoside derivatives of ribonuclease A

Biochemistry ◽  
1990 ◽  
Vol 29 (4) ◽  
pp. 928-937 ◽  
Author(s):  
Joseph Nachman ◽  
Maria Miller ◽  
Gary L. Gilliland ◽  
Robert Carty ◽  
Matthew Pincus ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Ribonuclease A ◽  
Derivatives Of

Morpholino, Piperidino, and Pyrrolidino Derivatives of Pyrimidine Nucleosides as Inhibitors of Ribonuclease A: Synthesis, Biochemical, and Crystallographic Evaluation#,†

2009 ◽  
Vol 52 (4) ◽  
pp. 932-942 ◽  
Author(s):  
Anirban Samanta ◽  
Demetres D. Leonidas ◽  
Swagata Dasgupta ◽  
Tanmaya Pathak ◽  
Spyros E. Zographos ◽  
...  
Keyword(s):  
Ribonuclease A ◽  
Pyrimidine Nucleosides ◽  
Derivatives Of
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