Macromolecular composition of Xenopus laevis egg jelly coat

Biochemistry ◽  
1975 ◽  
Vol 14 (14) ◽  
pp. 3101-3107 ◽  
Author(s):  
Edward C. Yurewicz ◽  
Gene Oliphant ◽  
Jerry L. Hedrick
Keyword(s):  
Xenopus Laevis ◽  
Jelly Coat ◽  
Egg Jelly ◽  
Macromolecular Composition

scholarly journals O-glycan variability of egg-jelly mucins from Xenopus laevis: characterization of four phenotypes that differ by the terminal glycosylation of their mucins

2000 ◽  
Vol 352 (2) ◽  
pp. 449-463 ◽  
Author(s):  
Yann GUERARDEL ◽  
Ossarath KOL ◽  
Emmanuel MAES ◽  
Tony LEFEBVRE ◽  
Bénoni BOILLY ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Molecular Mass ◽  
Electrophoretic Analysis ◽  
High Molecular Mass ◽  
Methylation Analysis ◽  
Glycosylation Pattern ◽  
Jelly Coat ◽  
New Family ◽  
Egg Jelly

Eggs from Xenopus laevis are surrounded by several layers of jelly that are needed for proper fertilization. Jelly coat is composed of high-molecular-mass glycoconjugates to which are bound many globular proteins. O-glycans released from the jelly coat of X. laevis have been partially described in previous studies. In this study, we compared the glycosylation pattern of the egg jelly coat isolated from six specimens of X. laevis. The O-glycans were released from jelly coats by alkali/borohydride treatment. Structural characterization was performed through a combination of one- and two-dimensional 1H-NMR and methylation analysis. This allowed the description of a new family of sulphated O-glycans present in jelly coats of all X. laevis. However, the jelly O-glycans showed a low extent of polymorphism between specimens. This intra-specific variability was restricted to the terminal substitution of O-linked oligosaccharides. The differential expression of two glycosyltransferase [an α-(1 → 4) galactosyltransferase and an α-(1 → 3) fucosyltransferase] activities resulted in the characterization of four phenotypes of X. laevis. Furthermore, electrophoretic analysis suggested that the high-molecular-mass fraction of jelly coat was mostly composed of mucin-type glycoproteins. Blot analysis with lectins confirmed that the glycan variability was borne by these mucin-type components. However, fertilization assays suggested that the glycan polymorphism had no repercussion on egg fertilizability.

scholarly journals O-glycan variability of egg-jelly mucins from Xenopus laevis: characterization of four phenotypes that differ by the terminal glycosylation of their mucins

2000 ◽  
Vol 352 (2) ◽  
pp. 449 ◽  
Author(s):  
Yann GUERARDEL ◽  
Ossarath KOL ◽  
Emmanuel MAES ◽  
Tony LEFEBVRE ◽  
Bénoni BOILLY ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Egg Jelly

2,4-D Butoxyethyl Ester Kinetics in Embryos of Xenopus laevis: The Role of the Embryonic Jelly Coat in Reducing Chemical Absorption

2006 ◽  
Vol 52 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Andrea N. Edginton ◽  
Claude Rouleau ◽  
Gerald R. Stephenson ◽  
Herman J. Boermans
Keyword(s):  
Xenopus Laevis ◽  
Chemical Absorption ◽  
Jelly Coat

Visualization of the Lytechinus pictus egg jelly coat in platinum replicas

1990 ◽  
Vol 105 (1-3) ◽  
pp. 123-132 ◽  
Author(s):  
Barry Bonnell ◽  
Douglas Chandler
Keyword(s):  
Lytechinus Pictus ◽  
Jelly Coat ◽  
Egg Jelly

Structure of Four Acidic Oligosaccharides from the Jelly Coat Surrounding the Eggs of Xenopus Laevis

2008 ◽  
Vol 231 (2) ◽  
pp. 434-439
Author(s):  
Yves Plancke ◽  
Jean-Michel Wieruszeski ◽  
Catherine Alonso ◽  
Benoni Boilly ◽  
Gérard Strecker
Keyword(s):  
Xenopus Laevis ◽  
Jelly Coat

scholarly journals Distribution of Lectin Binding Sites in Xenopus laevis Egg Jelly

1999 ◽  
Vol 210 (2) ◽  
pp. 428-439 ◽  
Author(s):  
Nancy M. Mozingo ◽  
Jerry L. Hedrick
Keyword(s):  
Xenopus Laevis ◽  
Binding Sites ◽  
Lectin Binding ◽  
Egg Jelly ◽  
Lectin Binding Sites

Structures of the sugar chains of a major glycoprotein present in the egg jelly coat of a starfish, Asterias amurensis

1987 ◽  
Vol 252 (1) ◽  
pp. 105-112 ◽  
Author(s):  
Tamao Endo ◽  
Motonori Hoshi ◽  
Satoshi Endo ◽  
Yoji Arata ◽  
Akira Kobata
Keyword(s):  
Asterias Amurensis ◽  
Jelly Coat ◽  
Egg Jelly ◽  
Sugar Chains
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