Cell surface changes correlated with density-dependent growth inhibition. Glycosaminoglycan metabolism in 3T3, SV3T3, and Con A selected revertant cells

Biochemistry ◽  
1975 ◽  
Vol 14 (2) ◽  
pp. 347-357 ◽  
Author(s):  
Richard Roblin ◽  
Sharon O. Albert ◽  
Nancy A. Gelb ◽  
Paul H. Black
1984 ◽  
Vol 70 (2) ◽  
pp. 127-130 ◽  
Author(s):  
Nityagopal Chakraborty ◽  
Satya Ranjan Bose ◽  
Jayasree Roy Chowdhury

Phytohemagglutinin (PHA) and concanavalin A (Con A) were used as probes to detect changes in the cell surface of Dalton's lymphoma, sarcoma-180 and Ehrlich's carcinoma after short in vitro exposure to acriflavine. Dye-treated cells showed enhancement of agglutination both by PHA and Con A, and such enhancement was found to be dependent on the time of exposure and concentration of acriflavine. However, PHA-induced percent agglutination seemed to be much higher than that of Con A among the 3 cell types. There were also marked differences among the 3 cell types in order of their sensitivity to lectin-mediated agglutination. The strength of the response was greater in lymphoma to both PHA and Con A than that of sarcoma-180 and carcinoma cells, which appeared to be most resistant. Acriflavine, which is known as an intercalative agent with DNA, induces cell surface changes by promoting lectin-mediated cellular agglutination.


1984 ◽  
Vol 28 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Anders Lundquist ◽  
Bertil Löwkvist

Nature ◽  
1974 ◽  
Vol 250 (5469) ◽  
pp. 739-741 ◽  
Author(s):  
IIH-NAN CHOU ◽  
PAUL H. BLACK ◽  
RICHARD O. ROBLIN

1980 ◽  
Vol 127 (1) ◽  
pp. 143-157 ◽  
Author(s):  
Shinichi Aizawa ◽  
Youji Mitsui ◽  
Fumiko Kurimoto ◽  
Kohji Nomura

1974 ◽  
Vol 39 (0) ◽  
pp. 1181-1185 ◽  
Author(s):  
H. Bauer ◽  
R. Kurth ◽  
L. Rohrschneider ◽  
G. Pauli ◽  
R. R. Friis ◽  
...  

1997 ◽  
Vol 52 (1-2) ◽  
pp. 33-41
Author(s):  
Angelika Preisfeld ◽  
Gabriele Scholten-Beck ◽  
Hans Georg Ruppel

Abstract Biochemical and ultrastructural examinations on the pellicle of autotrophically grown Eu­glena gracilis were carried out after three days under potassium and magnesium deficiency. Cell-surface changes were detected by lectin assay. Compared to cells grown in complete medium, deficient cells become larger in shape, accompanied by rising carbohydrate, chloro­phyll and protein content, bind more and other lectin molecules: an increase of mainly galactose and N-acetylgalactosamine receptors was observed. Investigations with the mucilage stains alcian blue and ruthenium red indicated that mucilaginous material is released under deficient conditions, whereas the control cells show a strong precipitate of these stains well inside the cells beneath the pellicle.


1981 ◽  
Vol 190 (6) ◽  
pp. 313-319 ◽  
Author(s):  
Kiyoko Yamazaki Yamamoto ◽  
Ruby Ozawa ◽  
Kenzo Takata ◽  
Junzoh Kitoh

1979 ◽  
Vol 6 (5) ◽  
pp. 347-363 ◽  
Author(s):  
AUREL P. LUPULESCU ◽  
JOHN M. CHADWICK ◽  
THOMAS F. DOWNHAM

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