Purification of adenovirus messenger ribonucleic acid by an aqueous polymer two-phase system

Biochemistry ◽  
1976 ◽  
Vol 15 (26) ◽  
pp. 5754-5761 ◽  
Author(s):  
Stanley Mak ◽  
Bo Oberg ◽  
Katarina Johansson ◽  
Lennart Philipson
Keyword(s):  
Ribonucleic Acid ◽  
Phase System ◽  
Two Phase ◽  
Messenger Ribonucleic Acid ◽  
Aqueous Polymer

scholarly journals Extraction and Purification of Capsaicin from Capsicum Oleoresin Using a Combination of Tunable Aqueous Polymer-Phase Impregnated Resin (TAPPIR) Extraction and Chromatography Technology

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3956 ◽  
Author(s):  
Lu ◽  
Cui
Keyword(s):  
Sodium Citrate ◽  
Extraction Process ◽  
Fractional Factorial ◽  
Phase System ◽  
Polymer Phase ◽  
Macroporous Resin ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Extraction And Purification ◽  
Extraction Step

Capsaicin, which mainly comes from pepper, exhibits anticancer, antioxidant, and anti-obesity properties. This work aims to construct a comprehensive technology for the extraction and purification of capsaicin from capsicum oleoresin. The tunable aqueous polymer phase impregnated HZ816 resins were selected in extraction step. In the extraction process, 3 g of impregnated HZ816 macroporous resin was employed per system. The results showed that a higher molecular weight of Polyethylene glycol (PEG) and 1-ethyl-3-methyl imidazolium acetate ([Emim] [OAc]) are more beneficial to the improvement of the yield of capsaicin. Screening experiment using fractional factorial designs indicated that the amount of sample loading, pH, and concentration of [Emim] [OAc] and PEG 6000 significantly affect the yield of capsaicin. Mathematical models of capsaicin yield in tunable aqueous polymer-phase impregnated resins were established and optimum condition was obtained using response surface methodology. The optimum impregnated phase was the polymer phase of an aqueous two-phase system which contained 18.5% (w/w) PEG6000, 15% (w/w) sodium citrate, and 10% (w/w) [Emim] [OAc] at pH 6.5. Under the optimal conditions, the yield of capsaicin reached 95.82% when the extraction system contains 0.25 g capsicum oleoresin. Ultimately, capsaicinoids extract was purified by reverse-phase resin (SKP-10-4300) chromatographic column. The capsaicin recovery and purity achieved 85% and 92%, respectively.

PREPARATION AND CHARACTERIZATION OF INTACT AND FREE SPORES OF BACILLUS POPILLIAE DUTKY

1967 ◽  
Vol 13 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Grant St. Julian ◽  
Thomas G. Pridham ◽  
Harlow H. Hall
Keyword(s):  
Malachite Green ◽  
Potassium Phosphate ◽  
Staining Technique ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer ◽  
Sonic Treatment ◽  
Bacillus Popilliae ◽  
Almost All

Spores of Bacillus popilliae are enclosed within a large sporangium. In an effort to characterize the spores precisely, a method was developed to free them from their sporangia by sonic vibration. Separation of the free spores from sporangial debris was made by lysozyme treatment, centrifugation, and the Sacks and Alderton aqueous polymer two-phase system. The intact spores are fully refractile; they accept and retain malachite green applied by a modified Wirtz spore-staining technique. The free spores are elongated with rounded ends and measure 1.30–2.25 μ × 0.90–1.40 μ. Outgrowth of intact germinated spores is lowest when heat-activated at 80 °C and highest when heat-activated at 50 °C. Free spores are more heat sensitive than are intact spores, possibly because of damage by the sonic treatment. L-Alanine, adenosine, and glucose separately and in combination do not influence germination of either intact or free spores. Although potassium phosphate somewhat increased germination of intact spores, other salts were ineffective, but almost all salts increased germination of free spores.

A Device for Countercurrent Distribution of Particles by an Aqueous Polymer Two-Phase System

1978 ◽  
Vol 13 (9) ◽  
pp. 745-752 ◽  
Author(s):  
Hiroyuki Nakazawa ◽  
Takenori Tanimura ◽  
Zenzo Tamura
Keyword(s):  
Countercurrent Distribution ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

Purification of glucosyltransferase from cell-lysate of Streptococcus mutans by counter-current chromatography using aqueous polymer two-phase system

2004 ◽  
Vol 805 (1) ◽  
pp. 155-160 ◽  
Author(s):  
Akio Yanagida ◽  
Mitsuhiro Isozaki ◽  
Yoichi Shibusawa ◽  
Heisaburo Shindo ◽  
Yoichiro Ito
Keyword(s):  
Streptococcus Mutans ◽  
Phase System ◽  
Two Phase ◽  
Cell Lysate ◽  
Aqueous Polymer ◽  
Counter Current

Concentration of Poliovirus by an Aqueous Polymer Two-Phase System

Nature ◽  
1960 ◽  
Vol 188 (4755) ◽  
pp. 1047-1048 ◽  
Author(s):  
E. C. J. NORRBY ◽  
P. Å. ALBERTSSON
Keyword(s):  
Phase System ◽  
Two Phase ◽  
Aqueous Polymer

A comparison of six methods for the concentration of a porcine enterovirus

1977 ◽  
Vol 23 (8) ◽  
pp. 1052-1058 ◽  
Author(s):  
D. T. G. Hazlett
Keyword(s):  
Single Step ◽  
Phase System ◽  
Virus Concentration ◽  
Two Phase ◽  
Recovery Rates ◽  
Aqueous Polymer ◽  
Porcine Enterovirus ◽  
Concentration Factors ◽  
Polyethylene Glycol Precipitation ◽  
Original Virus

Single-step concentration of porcine enterovirus strain T80 by adsorption to the polyelectrolyte PE60 gave virus concentration factors of 35- to 88-fold in terms of plaque-forming units, with recovery rates of 22 to 75% of total virus present in the original virus suspension. Concentration by separation in an aqueous polymer two-phase system gave virus concentration factors of 56- to 105-fold and recovery rates of 37 to 107%. In the latter procedure, sodium dextran sulfate appeared to have no effect on plaque numbers, although plaques were sharper and clearer when this substance was incorporated in the overlay. The failure of sonication of virus concentrated by either procedure to increase plaque numbers indicated the absence of virus aggregates in the concentrates. T80 virus was not effectively concentrated by cobalt chloride or polyethylene glycol precipitation or by adsorption to either aluminium hydroxide or calcium hydrogen phosphate.

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