Methionyl-tRNA synthetase from Escherichia coli: active stoichiometry and stopped-flow analysis of methionyl adenylate formation

Biochemistry ◽  
1976 ◽  
Vol 15 (17) ◽  
pp. 3678-3685 ◽  
Author(s):  
Francois Hyafil ◽  
Yannick Jacques ◽  
Guy Fayat ◽  
Michel Fromant ◽  
Philippe Dessen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Flow Analysis ◽  
Trna Synthetase ◽  
Stopped Flow ◽  
Methionyl Trna Synthetase

scholarly journals Use of β3-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase

2020 ◽  
Vol 209 (2) ◽  
pp. 107435 ◽  
Author(s):  
Giuliano Nigro ◽  
Sophie Bourcier ◽  
Christine Lazennec-Schurdevin ◽  
Emmanuelle Schmitt ◽  
Philippe Marlière ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Trna Synthetase ◽  
Methionyl Trna Synthetase ◽  
Acid Substrate ◽  
Amino Acid Substrate

scholarly journals Methionyl-tRNA Synthetase from Escherichia coli

2005 ◽  
Vol 127 (3) ◽  
pp. 449-457 ◽  
Author(s):  
David G. BARKER ◽  
Jean-Pierre EBEL ◽  
Ross JAKES ◽  
Chris J. BRUTON
Keyword(s):  
Escherichia Coli ◽  
Trna Synthetase ◽  
Methionyl Trna Synthetase

A new method for the isolation of methionyl transfer RNA synthetase mutants from Escherichia coli

1975 ◽  
Vol 21 (6) ◽  
pp. 754-758 ◽  
Author(s):  
John B. Armstrong ◽  
John A. Fairfield
Keyword(s):  
Escherichia Coli ◽  
Trna Synthetase ◽  
Transfer Rna ◽  
New Method ◽  
Wild Type ◽  
E Coli ◽  
Adenosyl Methionine ◽  
K 12 ◽  
Methionyl Trna Synthetase

Six methionine auxotrophs were isolated from an E. coli K-12 strain which required up to 100 times as much methionine for growth as a conventional auxotroph. In these mutants, the methionyl-tRNA synthetase had an increased Km for methionine. The Km value for the mutants ranged from 0.48 to 1.63 mM, compared to 0.078 mM for the wild type. The Km (methionine) for S-adenosyl methionine synthetase was not altered.

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