Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems

Biochemistry ◽  
1976 ◽  
Vol 15 (14) ◽  
pp. 2959-2964 ◽  
Author(s):  
Harry Walter ◽  
Eugene J. Krob ◽  
Donald E. Brooks
Keyword(s):  
Surface Properties ◽  
Cell Separation ◽  
Membrane Surface ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Characterization and optimization of immunoaffinity aqueous two‐phase systems with PEGylated CD133/2‐biotin antibody in route to stem cell separation

2019 ◽  
Vol 95 (1) ◽  
pp. 123-131
Author(s):  
Alonso Ornelas‐González ◽  
Stefanie U Reisenauer ◽  
Mirna González‐González ◽  
Marco Rito‐Palomares
Keyword(s):  
Stem Cell ◽  
Cell Separation ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Surface heterogeneity of bovine sperm revealed by aqueous two-phase partition

1991 ◽  
Vol 11 (5) ◽  
pp. 265-273 ◽  
Author(s):  
Elizabeth J. Cartwright ◽  
Allison Cowin ◽  
Paul T. Sharpe
Keyword(s):  
Surface Properties ◽  
Seminal Fluid ◽  
Surface Heterogeneity ◽  
Two Phase ◽  
Surface Molecules ◽  
Affinity Ligand ◽  
Bovine Sperm ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Two Phase Partition

Ejaculated, bovine sperm have been subjected to multiple partition in aqueous two-phase systems. This partition, carried out in a countercurrent fashion, reveals heterogeneity of the sperm population with respect to surface properties. The sperm, when partitioned in phase systems that detect non-change associated surface properties (change-insensitive) are largely distributed as two distinct populations. In charge-sensitive phase systems (which principally detect cell surface molecules carrying charge) the sperm do not show any obvious surface heterogeneity. Considerable heterogeneity is revealed in affinity-ligand phase systems containing palmitic acid coupled to one of the phase components-poly(ethylene glycol). There is a difference in surface heterogeneity between sperm which have been washed in buffer or left unwashed, direct from the ejaculate. This is indicative of weak adsorption of proteins to the sperm surface in seminal fluid. These results show that bovine ejaculated sperm is a heterogeneous cell population having unequal distributions of a number of different surface molecules.

Cell Separation in Aqueous Two-Phase Systems − Influence of Polymer Molecular Weight and Tie-Line Length on the Resolution of Five Model Cell Lines

2017 ◽  
Vol 13 (2) ◽  
pp. 1700250 ◽  
Author(s):  
Sarah Zimmermann ◽  
Sarah Gretzinger ◽  
Philipp K. Zimmermann ◽  
Are Bogsnes ◽  
Mattias Hansson ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cell Lines ◽  
Cell Separation ◽  
Line Length ◽  
Polymer Molecular Weight ◽  
Two Phase ◽  
Model Cell ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tie Line

scholarly journals Characterization of Surface Properties of Heat Shock Proteins for the Separation Using Aqueous Two-Phase Systems.

1994 ◽  
Vol 27 (6) ◽  
pp. 808-814 ◽  
Author(s):  
Koji Yano ◽  
Tetsuhiro Hasegawa ◽  
Ryoichi Kuboi ◽  
Isao Komasawa ◽  
Tetsuaki Tsuchido
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Surface Properties ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Shock Proteins

Aqueous two-phase systems - a label-free tech-nology for cell separation

Cytotherapy ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. S45 ◽  
Author(s):  
S. Nagel ◽  
S. Gretzinger ◽  
M. Schwab ◽  
S.A. Oelmeier ◽  
F. Dismer ◽  
...  
Keyword(s):  
Cell Separation ◽  
Label Free ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

scholarly journals Differentiation of surface properties of chlorococcalean algae by means of aqueous two phase systems

2014 ◽  
Vol 58 (1) ◽  
pp. 59-64
Author(s):  
Jan Burczyk ◽  
Dorota Szymakowska
Keyword(s):  
Surface Properties ◽  
Two Phase ◽  
Ph Values ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Algal cells belonging to various strains of <em>Chlorococcales (Chlorophyta) </em>have been partitioned in aqueous two-phase systems containing ionogenic polymers, DEAE-dextran or SDS-dextran, at various pH values. Strain-specific differences of partition type which have been found in the phase systems used can be useful for distinguishing of algal cells.

Sign in / Sign up

Export Citation Format

Share Document