Microviscosity of the hydrocarbon region of the bovine retinal rod outer segment disk membrane determined by fluorescent probe measurements

Biochemistry ◽  
1976 ◽  
Vol 15 (13) ◽  
pp. 2766-2772 ◽  
Author(s):  
Gene W. Stubbs ◽  
Burton J. Litman ◽  
Yechezkel Barenholz
Keyword(s):  
Fluorescent Probe ◽  
Outer Segment ◽  
Rod Outer Segment ◽  
Retinal Rod ◽  
Disk Membrane ◽  
Probe Measurements

Differential membrane protein phosphorylation in bovine retinal rod outer segment disk membranes as a function of disk age

1996 ◽  
Vol 16 (4) ◽  
pp. 289-297 ◽  
Author(s):  
Kathleen Boesze-Battaglia ◽  
Arlene D. Albert ◽  
James S. Frye ◽  
Philip L. Yeagle
Keyword(s):  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Phosphorylation ◽  
Outer Segment ◽  
Cholesterol Content ◽  
Membrane Cholesterol ◽  
Magic Angle ◽  
Rod Outer Segment ◽  
Retinal Rod ◽  
Disk Membrane

The outer segment portion of photoreceptor rod cells is composed of a stacked array of disk membranes. Newly formed disks are found at the base of the rod outer segment (ROS) and are relatively high in membrane cholesterol. Older disks are found at the apical tip of the ROS and are low in membrane cholesterol. Disk membranes were separated based on their membrane cholesterol content and the extent of membrane protein phosphorylation determined. Light induced phosphorylation of ROS disk membrane proteins was investigated using magic angle spinning31P NMR. When intact rod outer segment preparations were stimulated by light, in the presence of endogenously available kinases, membrane proteins located in disks at the base of the ROS were more heavily phosphorylated than those at the tip. SDS-gel electrophoresis of the phosphorylated disk membranes subpopulations identified a phosphoprotein species with a molecular weight of approximately 68–72 kDa that was more heavily phosphorylated in newly formed disks than in old disks. The identity of this phosphoprotein is presently under investigation. When the phosphorylation reaction was carried out in isolated disk membrane preparations with exogenously added co-factors and kinases, there was no preferential protein phosphorylation. Taken collectively, these results suggest that within the ROS there is a protein phosphorylation gradient that maybe indicative of co-factor or kinase heterogeneity.

Disaturated and dipolyunsaturated phospholipids in the bovine retinal rod outer segment disk membrane

1979 ◽  
Vol 552 (2) ◽  
pp. 294-306 ◽  
Author(s):  
George P. Miljanich ◽  
Larry A. Sklar ◽  
Drina L. White ◽  
Edward A. Dratz
Keyword(s):  
Outer Segment ◽  
Rod Outer Segment ◽  
Retinal Rod ◽  
Disk Membrane

Guanylate Cyclase of Retinal Rod Outer Segment

1990 ◽  
pp. 139-145
Author(s):  
I. M. Pepe ◽  
I. Panfoli ◽  
C. Cugnoli
Keyword(s):  
Guanylate Cyclase ◽  
Outer Segment ◽  
Rod Outer Segment ◽  
Retinal Rod

Evidence for aerobic metabolism in retinal rod outer segment disks

2009 ◽  
Vol 41 (12) ◽  
pp. 2555-2565 ◽  
Author(s):  
Isabella Panfoli ◽  
Daniela Calzia ◽  
Paolo Bianchini ◽  
Silvia Ravera ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Outer Segment ◽  
Aerobic Metabolism ◽  
Rod Outer Segment ◽  
Retinal Rod

Fusion between Retinal Rod Outer Segment Membranes and Model Membranes:  A Role for Photoreceptor Peripherin/rds†

Biochemistry ◽  
1998 ◽  
Vol 37 (26) ◽  
pp. 9477-9487 ◽  
Author(s):  
Kathleen Boesze-Battaglia ◽  
Om P. Lamba ◽  
Andrew A. Napoli, ◽  
Santosh Sinha ◽  
Yuqing Guo
Keyword(s):  
Outer Segment ◽  
Model Membranes ◽  
Rod Outer Segment ◽  
Retinal Rod

Topology of amino phospholipids in bovine retinal rod outer segment disk membranes

Biochemistry ◽  
1978 ◽  
Vol 17 (20) ◽  
pp. 4186-4192 ◽  
Author(s):  
Richard C. Crain ◽  
Guido V. Marinetti ◽  
David F. O'Brien
Keyword(s):  
Outer Segment ◽  
Rod Outer Segment ◽  
Retinal Rod
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