High molecular weight deoxyribonucleic acid polymerase from crown gall tumor cells of periwinkle (Vinca rosea)

Biochemistry ◽  
1976 ◽  
Vol 15 (3) ◽  
pp. 688-697 ◽  
Author(s):  
John M. Gardner ◽  
Clarence I. Kado
Planta ◽  
1979 ◽  
Vol 146 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Constance Bouricius Lentz ◽  
Thomas K. Hodges ◽  
Ann G. Matthysse

1975 ◽  
Vol 149 (1) ◽  
pp. 289-291 ◽  
Author(s):  
G M Blackburn ◽  
P E Taussig

Anthracene becomes covalently bound to high-molecular-weight DNA in mammalian tissue culture as a result of irradiation at 365 nm after the incubation of cells with the hydrocarbon. At high radiation doses, the extent of binding exceeds one hydrocarbon molecule per 103 bases, and is lethal. At low radiation doses, much decreased binding is observed, but a majority of cells remain viable and can be recultured.


1976 ◽  
Vol 15 (10) ◽  
pp. 1565 ◽  
Author(s):  
Chong-Maw Chen ◽  
Richard L. Eckert ◽  
James D. McChesney

1976 ◽  
Vol 73 (10) ◽  
pp. 3562-3564 ◽  
Author(s):  
R. Turgeon ◽  
H. N. Wood ◽  
A. C. Braun

1974 ◽  
Vol 2 (5) ◽  
pp. 864-865 ◽  
Author(s):  
ANDREW M. HOLMES ◽  
IAN P. HESSLEWOOD ◽  
WILLIAM F. WAKELING ◽  
IRVING R. JOHNSTON

1974 ◽  
Vol 137 (3) ◽  
pp. 543-546
Author(s):  
G. R. Barker ◽  
P. Hodges

1. Native DNA from two strains of Bacillus subtilis was chromatographed by stepwise elution from MAK (methylated albumin on kieselguhr). 2. Transforming activity was confined to two out of the three main fractions, activity being distributed between the two peaks differently for DNA from the different strains. 3. Fractionation of DNA from both strains on 2% agarose gel gave two components. Approx. 75% of the material was eluted within the void volume of the column. Approx. 25% of the material consisted of degradation products of lower molecular weight. 4. Chromatography on MAK of the material of high molecular weight eluted from agarose gel gave a number of peaks differing in molecular weight, indicating that degradation of the DNA takes place during chromatography on MAK. 5. The distribution of transforming activity among the fractions from MAK suggests that degradation occurs preferentially in certain regions of the DNA.


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