Subunit interactions in the methionyl-tRNA synthetase of Bacillus stearothermophilus

Biochemistry ◽  
1976 ◽  
Vol 15 (2) ◽  
pp. 243-249 ◽  
Author(s):  
Roderick S. Mulvey ◽  
Alan R. Fersht
Keyword(s):  
Bacillus Stearothermophilus ◽  
Trna Synthetase ◽  
Subunit Interactions ◽  
Methionyl Trna Synthetase

scholarly journals Probing the substrate-binding sites of aminoacyl-tRNA synthetases with the procion dye green HE-4BD

1989 ◽  
Vol 258 (3) ◽  
pp. 715-721 ◽  
Author(s):  
J E C McArdell ◽  
M Duffield ◽  
T Atkinson
Keyword(s):  
Bacillus Stearothermophilus ◽  
Competitive Inhibitor ◽  
Reactive Dye ◽  
Trna Synthetase ◽  
Enzyme Inactivation ◽  
Trna Synthetases ◽  
Time Period ◽  
Substrate Binding Sites ◽  
Methionyl Trna Synthetase ◽  
Fold Excess

A reactive bis-dichloro derivative of the Procion dye Green HE-4BD was shown to inactivate irreversibly methionyl-tRNA synthetase (MTS) from Escherichia coli and also tryptophyl-tRNA synthetase (WTS) and tyrosyl-tRNA synthetase (YTS) from Bacillus stearothermophilus at pH 8.5 and 37 degrees C. At a 5-fold excess of reactive dye over enzyme subunit concentration MTS was quantitatively inactivated within 20 min in the ATP/pyrophosphate exchange assay, whereas WTS and YTS show an 80% loss of activity over the same time period. The inactivation is affected by the addition of substrates, which either protect (WTS and YTS) or promote (YTS with tyrosine) the dye-mediated enzyme inactivation. Green HE-4BD-OH was shown to be a competitive inhibitor of MTS with respect to MgATP, methionine and tRNA substrates.

scholarly journals A MUTANT OF YEAST WITH A DEFECTIVE METHIONYL-tRNA SYNTHETASE

Genetics ◽  
1969 ◽  
Vol 61 (3) ◽  
pp. 557-566
Author(s):  
Calvin S McLaughlin ◽  
Leland H Hartwell
Keyword(s):  
Trna Synthetase ◽  
Methionyl Trna Synthetase

Cloning and complete nucleotide sequence of the Bacillus stearothermophilus tryptophanyi tRNA synthetase gene

Gene ◽  
1986 ◽  
Vol 46 (1) ◽  
pp. 37-45 ◽  
Author(s):  
David A. Barstow ◽  
Andy F. Sharman ◽  
Tony Atkinson ◽  
Nigel P. Minton
Keyword(s):  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Bacillus Stearothermophilus ◽  
Trna Synthetase
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