Diet-induced changes in plasma membrane fatty acid composition affect physical properties detected with a spin-label probe

Biochemistry ◽  
1977 ◽  
Vol 16 (24) ◽  
pp. 5280-5285 ◽  
Author(s):  
Mary Ellen King ◽  
Bruce W. Stavens ◽  
Arthur A. Spector
Keyword(s):  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Physical Properties ◽  
Acid Composition ◽  
Spin Label ◽  
Membrane Fatty Acid ◽  
Membrane Fatty Acid Composition ◽  
Label Probe ◽  
Induced Changes

Influence of altered plasma membrane fatty acid composition on cesium transport characteristics and toxicity inSaccharomyces cerevisiae

1997 ◽  
Vol 43 (10) ◽  
pp. 954-962 ◽  
Author(s):  
Michael J. Hoptroff ◽  
Simon V. Avery ◽  
Simon Thomas
Keyword(s):  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Acid Composition ◽  
Protein Interactions ◽  
Kinetic Studies ◽  
Monovalent Cation ◽  
Membrane Fatty Acid ◽  
Membrane Fatty Acid Composition ◽  
Prolonged Incubation

The influence of altered plasma membrane fatty acid composition on cesium uptake and toxicity was investigated in Saccharomyces cerevisiae. Detailed kinetic studies revealed that both the Vmaxand Kmvalues for Cs+transport increased (by approximately twofold in the latter case) when S. cerevisiae was grown in medium supplemented with the polyunsaturated fatty acid linoleate. In addition, Cs+uptake by linoleate-enriched cells was considerably less sensitive to the competitive effects of other monovalent cations (K+, Rb+, and NH4+) than that by unsupplemented cells. Stimulation of Cs+uptake in the presence of certain K+and Rb+concentrations was only evident in linoleate-enriched S. cerevisiae. At 100 mM CsCl, the initial rate of Cs+uptake was greater in linoleate-supplemented cells than in unsupplemented cells and this was reflected in a more rapid displacement of cellular K+. However, little difference in net Cs+accumulation between linoleate-supplemented and unsupplemented cells was evident during prolonged incubation in buffer or during growth. Thus, Cs+toxicity was similar in linoleate-supplemented and unsupplemented cells. The results were consistent with the Cs+(K+) transport mechanism adopting an altered conformational state in linoleate-enriched S. cerevisiae.Key words: monovalent cation transport, plasma membrane fatty acid composition, lipid–protein interactions, metal–microbe interactions, cation competition.

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